Western Blot (WB)
(All lanes: Histone-lysine N-methyltransferase SMYD1 antibody at 2ug/mlLane 1:mouse heart tissueLane 2:293T whole cell lysateLane 3:mouse skeletal muscle tissueSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 57kDaObserved band size: 57kDa)
Rabbit anti-Human, Mouse Histone-lysine N-methyltransferase SMYD1 Polyclonal Antibody | anti-SMYD1 antibody
Rabbit anti-human Histone-lysine N-methyltransferase SMYD1 polyclonal Antibody(SMYD1)
Gene Names
SMYD1; BOP; KMT3D; ZMYND18; ZMYND22
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Antigen Affinity Purified
Synonyms
Histone-lysine N-methyltransferase SMYD1; Polyclonal Antibody; Rabbit anti-human Histone-lysine N-methyltransferase SMYD1 polyclonal Antibody(SMYD1); SET and MYND domain-containing protein 1; SMYD1; anti-SMYD1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen Affinity Purified
Sequence Length
490
Applicable Applications for anti-SMYD1 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Recommended dilution: WB:1:500-2000
IHC:1:20-1:200
IHC:1:20-1:200
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Conjugate
Non-conjugated
Immunogen
Recombinant human Histone-lysine N-methyltransferase SMYD1 protein (1-350aa)
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-79080
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.
Western Blot (WB)
(All lanes: Histone-lysine N-methyltransferase SMYD1 antibody at 2ug/mlLane 1:mouse heart tissueLane 2:293T whole cell lysateLane 3:mouse skeletal muscle tissueSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 57kDaObserved band size: 57kDa)
Western Blot (WB)
(All lanes: Histone-lysine N-methyltransferase SMYD1 antibody at 2ug/mlLane 1:mouse heart tissueLane 2:293T whole cell lysateLane 3:mouse skeletal muscle tissueSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 57kDaObserved band size: 57kDa)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human lung tissue using MBS1493593 at dilution 1:100)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human lung tissue using MBS1493593 at dilution 1:100)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human spleen tissue using MBS1493593 at dilution 1:100)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human spleen tissue using MBS1493593 at dilution 1:100)
Related Product Information for anti-SMYD1 antibody
Methylates histone H3 at 'Lys-4' (H3K4me), seems able to perform both mono-, di-, and trimethylation. Acts as a transcriptional repressor. Essential for cardiomyocyte differentiation and cardiac morphogenesis.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
56,617 Da
NCBI Official Full Name
histone-lysine N-methyltransferase SMYD1
NCBI Official Synonym Full Names
SET and MYND domain containing 1
NCBI Official Symbol
SMYD1
NCBI Official Synonym Symbols
BOP; KMT3D; ZMYND18; ZMYND22
NCBI Protein Information
histone-lysine N-methyltransferase SMYD1
UniProt Protein Name
Histone-lysine N-methyltransferase SMYD1
Protein Family
UniProt Gene Name
SMYD1
UniProt Entry Name
SMYD1_HUMAN
Uniprot Description
SMYD1: Acts as a transcriptional repressor. Essential for cardiomyocyte differentiation and cardiac morphogenesis.
Protein type: Methyltransferase, protein lysine; EC 2.1.1.43; Transcription, coactivator/corepressor
Chromosomal Location of Human Ortholog: 2p11.2
Cellular Component: cytoplasm; nucleus
Molecular Function: DNA binding; histone-lysine N-methyltransferase activity; metal ion binding; protein binding; transcription corepressor activity
Biological Process: chromatin remodeling; heart development; negative regulation of transcription, DNA-dependent; positive regulation of myoblast differentiation; transcription, DNA-dependent
Research Articles on SMYD1
Similar Products
Product Notes
The SMYD1 smyd1 (Catalog #AAA1493593) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-human Histone-lysine N-methyltransferase SMYD1 polyclonal Antibody(SMYD1) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Histone-lysine N-methyltransferase SMYD1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Recommended dilution: WB:1:500-2000 IHC:1:20-1:200. Researchers should empirically determine the suitability of the SMYD1 smyd1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone-lysine N-methyltransferase SMYD1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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