Western Blot (WB)
(Western blotAll lanes: Target of rapamycin complex 2 subunit MAPKAP1 antibody at 2ug/mlLane 1:Hela whole cell lysateLane 2:MCF-7 whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 60,55,54,38,37,42 kDaObserved band size: 59 kDa)
Rabbit anti-Human Target of rapamycin complex 2 subunit MAPKAP1 Polyclonal Antibody | anti-MAPKAP1 antibody
Rabbit anti-human Target of rapamycin complex 2 subunit MAPKAP1 polyclonal Antibody(MAPKAP1)
Western Blot (WB)
(Western blotAll lanes: Target of rapamycin complex 2 subunit MAPKAP1 antibody at 2ug/mlLane 1:Hela whole cell lysateLane 2:MCF-7 whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 60,55,54,38,37,42 kDaObserved band size: 59 kDa)
Western Blot (WB)
(Western blotAll lanes: Target of rapamycin complex 2 subunit MAPKAP1 antibody at 2ug/mlLane 1:Hela whole cell lysateLane 2:MCF-7 whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 60,55,54,38,37,42 kDaObserved band size: 59 kDa)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a protein that is highly similar to the yeast SIN1 protein, a stress-activated protein kinase. Alternatively spliced transcript variants encoding distinct isoforms have been described. Alternate polyadenylation sites as well as alternate 3' UTRs have been identified for transcripts of this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
Sin1: a member of a conserved family of proteins that have an essential roles in signal transduction from yeast to mammals. An essential component of the mTORC2 complex, which consists of mTOR, LST8, Sin1, Protor (PRR5), and RICTOR, the Rapamycin Insensitive Companion of mTOR. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 regulates the phosphorylation of SGK1 S422 and PKCA S657. Enhances osmotic stress-induced phosphorylation of ATF2 and ATF2-mediated transcription. Contains a Ras-binding (RBD) and a pleckstrin homology (PH) domains. Plays an important role in the SAPK signaling pathway by binding directly to both ATF-2 and p38. A genetic knockout of Sin1 abolished Akt-S473 phosphorylation and disrupts the RICTOR-mTOR interaction, but Akt-Thr308 phosphorylation is maintained. Six alternatively-spliced isoforms of the human protein have been reported. All isoforms except 4 can be incorporated into the the mTORC2 complex.
Protein type: Adaptor/scaffold
Chromosomal Location of Human Ortholog: 9q33.3
Cellular Component: cytoplasm; cytoplasmic membrane-bound vesicle; cytosol; Golgi apparatus; nucleoplasm; nucleus; plasma membrane; TORC2 complex
Molecular Function: phosphatidylinositol-3,4,5-triphosphate binding; phosphatidylinositol-3,4-bisphosphate binding; phosphatidylinositol-4,5-bisphosphate binding; protein binding; protein kinase binding; Ras GTPase binding
Biological Process: epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; innate immune response; negative regulation of Ras protein signal transduction; nerve growth factor receptor signaling pathway; phosphoinositide-mediated signaling; substantia nigra development; T cell costimulation; vascular endothelial growth factor receptor signaling pathway
Research Articles on MAPKAP1
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Product Notes
The MAPKAP1 mapkap1 (Catalog #AAA1492926) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-human Target of rapamycin complex 2 subunit MAPKAP1 polyclonal Antibody(MAPKAP1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Target of rapamycin complex 2 subunit MAPKAP1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). Recommended dilution: WB:1:500-2000. Researchers should empirically determine the suitability of the MAPKAP1 mapkap1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Target of rapamycin complex 2 subunit MAPKAP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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