Rabbit anti-Human, Rat HS2ST1 Polyclonal Antibody | anti-HS2ST1 antibody

HS2ST1 Antibody

Cat. Num. AAA1491783

• Gene Names: HS2ST1; dJ604K5.2
• Reactivity: Human, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: >95%, Protein G purified
• Synonyms: HS2ST1; Polyclonal Antibody; HS2ST1 Antibody; Heparan sulfate 2-O-sulfotransferase 1 (EC:2.8.2.-); 2-O-sulfotransferase; 2OST; HS2ST; KIAA0448; anti-HS2ST1 antibody

• Host: Rabbit
• Reactivity: Human, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid
• Sequence Length: 229

• Applicable Applications for anti-HS2ST1 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB:1:500-1:5000; IHC:1:20-1:200
• Immunogen: Recombinant Human Heparan sulfate 2-O-sulfotransferase 1 protein (29-229AA)
• Storage Buffer: Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Conjugate: Non-conjugated
• Species: Human
• Research Area: Signal Transduction
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-103549 / sc-130779 / sc-292203
• Preparation and Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Western Blot (WB)

(Western blotAll lanes: HS2ST1 antibody at 6ug/mlLane 1:mouse skeletal muscle tissueLane 2:mouse heart tissueLane 3:PC-3 whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 42,35,27 kDaObserved band size: 42 kDa)

Western Blot (WB)

(Western BlotPositive WB detected in: Rat spleen tissueAll lanes: HS2ST1 antibody at 2.7ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 42,35,27 KDaObserved band size: 42 KDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human pancreatic tissue using MBS1491783 at dilution of 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human lung cancer using MBS1491783 at dilution of 1:100)

Related Product Information for anti-HS2ST1 antibody

Catalyzes the transfer of sulfate to the C2-position of selected hexuronic acid residues within the maturing heparan sulfate (HS). 2-O-sulfation within HS, particularly of iduronate residues, is essential for HS to participate in a variety of high-affinity ligand-binding interactions and signaling processes. Mediates 2-Osulfation of both L-iduronyl and D-glucuronyl residues (By similarity).

NCBI and Uniprot Product Information

• NCBI GI #: 197382758
• NCBI GeneID: 9653
• NCBI Accession #: NP_001127964.1
• NCBI GenBank Nucleotide #: NM_001134492.1
• UniProt Accession #: Q7LGA3; O75036; Q32NB5; Q8TAC5; Q9H441; Q9NUJ9; D3DT22
• NCBI Official Full Name: heparan sulfate 2-O-sulfotransferase 1 isoform 2
• NCBI Official Synonym Full Names: heparan sulfate 2-O-sulfotransferase 1
• NCBI Official Symbol: HS2ST1
• NCBI Official Synonym Symbols: dJ604K5.2
• NCBI Protein Information: heparan sulfate 2-O-sulfotransferase 1
• UniProt Protein Name: Heparan sulfate 2-O-sulfotransferase 1
• Protein Family: Heparan sulfate 2-O-sulfotransferase
• UniProt Gene Name: HS2ST1
• UniProt Synonym Gene Names: HS2ST; KIAA0448; 2-O-sulfotransferase; 2OST
• UniProt Entry Name: HS2ST_HUMAN

NCBI Description

Heparan sulfate biosynthetic enzymes are key components in generating a myriad of distinct heparan sulfate fine structures that carry out multiple biologic activities. This gene encodes a member of the heparan sulfate biosynthetic enzyme family that transfers sulfate to the 2 position of the iduronic acid residue of heparan sulfate. The disruption of this gene resulted in no kidney formation in knockout embryonic mice, indicating that the absence of this enzyme may interfere with the signaling required for kidney formation. Two alternatively spliced transcript variants that encode different proteins have been found for this gene. [provided by RefSeq, Aug 2008]

Uniprot Description

HS2ST1: Catalyzes the transfer of sulfate to the C2-position of selected hexuronic acid residues within the maturing heparan sulfate (HS). 2-O-sulfation within HS, particularly of iduronate residues, is essential for HS to participate in a variety of high- affinity ligand-binding interactions and signaling processes. Mediates 2-O-sulfation of both L-iduronyl and D-glucuronyl residues. Belongs to the sulfotransferase 3 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Glycan Metabolism - heparan sulfate biosynthesis; Transferase; EC 2.8.2.-; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p22.3

Cellular Component: Golgi membrane; integral to membrane; membrane

Molecular Function: sulfotransferase activity

Biological Process: carbohydrate metabolic process; glycosaminoglycan biosynthetic process; glycosaminoglycan metabolic process