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Type-1 restriction enzyme MjaXIP specificity protein (MJ0130) Recombinant Protein | MJ0130 recombinant protein

Recombinant Methanocaldococcus jannaschii Type-1 restriction enzyme MjaXIP specificity protein (MJ0130)

Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Synonyms
Type-1 restriction enzyme MjaXIP specificity protein (MJ0130); Recombinant Methanocaldococcus jannaschii Type-1 restriction enzyme MjaXIP specificity protein (MJ0130); Type-1 restriction enzyme MjaXIP specificity protein; S.MjaXIP; Type I restriction enzyme MjaXIP specificity protein; S protein; MJ0130 recombinant protein
Ordering
For Research Use Only!
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE.
Form/Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Sequence Positions
1-425aa; full length protein
Sequence
MFYKEENFKK TEIGEIPEDW EIVELKDVCK KIKAGGTPKT SVEEYYKNGT IPFVKIEDITNSNKYLTNTK IKITEEGLNN SNAWIVPKNS VLFAMYGSIG ETAINKIEVA TNQAILGIIPKDNILESEFL YYILAKNKNY YSKLGMQTTQ KNLNAQIVKS FKIPLPPLEE QKQIAKILTKIDEGIEIIEK SINKLERIKK GLMHKLLTKG IGHSRFKKSE IGEIPEDWEV FEIKDIFEVKTGTTPSTKKS EYWENGEINW ITPLDLSRLN EKIYIGSSER KVTKIALEKC NLNLIPKGSIIISTRAPVGY VAVLTVESTF NQGCKGLFQK NNDSVNTEFY AYYLKFKKNL LENLSGGSTFKELSKSMLEN FKIPLPPLEE QKQIAKILSS VDKSIELKKQ KKEKLQRMKK KIMELLLTGKVRVKT
Sequence Length
425
Species
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Preparation and Storage
Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
48,568 Da
NCBI Official Full Name
Type I restriction-modification enzyme subunit S
NCBI Official Symbol
MJ_0130m
NCBI Protein Information
Type I restriction-modification enzyme subunit S
UniProt Protein Name
Type-1 restriction enzyme MjaXIP specificity protein
UniProt Gene Name
MJ0130
UniProt Synonym Gene Names
S.MjaXIP; S protein
UniProt Entry Name
T1S1_METJA

Uniprot Description

The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to the same target sequence but cutting the DNA some distance from this site. Whether the DNA is cut or modified depends on the methylation state of the target sequence. When the target site is unmodified, the DNA is cut. When the target site is hemimethylated, the complex acts as a maintenance MTase modifying the DNA so that both strands become methylated. Subunit S dictates DNA sequences specificity ().

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Product Notes

The MJ0130 mj0130 (Catalog #AAA1486205) is a Recombinant Protein produced from E Coli or Yeast or Baculovirus or Mammalian Cell and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-425aa; full length protein. The amino acid sequence is listed below: MFYKEENFKK TEIGEIPEDW EIVELKDVCK KIKAGGTPKT SVEEYYKNGT IPFVKIEDIT NSNKYLTNTK IKITEEGLNN SNAWIVPKNS VLFAMYGSIG ETAINKIEVA TNQAILGIIP KDNILESEFL YYILAKNKNY YSKLGMQTTQ KNLNAQIVKS FKIPLPPLEE QKQIAKILTK IDEGIEIIEK SINKLERIKK GLMHKLLTKG IGHSRFKKSE IGEIPEDWEV FEIKDIFEVK TGTTPSTKKS EYWENGEINW ITPLDLSRLN EKIYIGSSER KVTKIALEKC NLNLIPKGSI IISTRAPVGY VAVLTVESTF NQGCKGLFQK NNDSVNTEFY AYYLKFKKNL LENLSGGSTF KELSKSMLEN FKIPLPPLEE QKQIAKILSS VDKSIELKKQ KKEKLQRMKK KIMELLLTGK VRVKT. It is sometimes possible for the material contained within the vial of "Type-1 restriction enzyme MjaXIP specificity protein (MJ0130), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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