Rabbit anti-Human IDDM12 Polyclonal Antibody

IDDM12, NT (CTLA4, CD152, Cytotoxic T-lymphocyte protein 4, Cytotoxic T-lymphocyte-associated antigen 4, CD152)

Cat. Num. AAA647624

• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: IDDM12; Polyclonal Antibody; NT (CTLA4; CD152; Cytotoxic T-lymphocyte protein 4; Cytotoxic T-lymphocyte-associated antigen 4; CD152); Anti -IDDM12; anti-IDDM12 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-IDDM12 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Immunohistochemistry: 1:50-100
• Immunogen: IDDM12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 57-86 amino acids from the N-terminal region of human IDDM12.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Testing Data

(All lanes: MBS647624 (1:2000) Lane 1: Raji whole cell lysate Lane 2: U266B1 whole cell lysate Lane 3: K562 whole cell lysate Lane 4: Jurkat whole cell lysate Lane 5: CCRF-CEM whole cell lysate Lane 6: Human spleen lysate Lysates/proteins at 20ug/ lane. Secondary Goat Anti-Rabbit IgG, (H+L) (HRP) (1:10,000). Predicted band size: 25kD Blocking/Dilution buffer: 5% NFDM/TBST.)

Testing Data

(All lanes: MBS647624 (1:2000) Lane 1: WiDr whole cell lysates Lane 2: Raji whole cell lysates Lane 3: K562 whole cell lysates Lane 4: Jurkat whole cell lysates. Lysates/proteins at (20ug/lane). Secondary Goat Anti-Rabbit IgG, (H+L) (HRP) (1:10,000). Predicted band size: 25kD Blocking/Dilution buffer: 5% NFDM/TBST.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma using MBS647624 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS647624 for immunohistochemistry.)

Related Product Information for anti-IDDM12 antibody

CTLA4 is a member of the immunoglobulin superfamily and encodes a protein which transmits an inhibitory signal to T cells. The protein contains a V domain, a transmembrane domain, and a cytoplasmic tail. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond, while the soluble isoform functions as a monomer. Mutations in this gene have been associated with insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, thyroid-associated orbitopathy, and other autoimmune diseases.

Product Categories/Family for anti-IDDM12 antibody

Antibodies; Abs to CD Markers