Equine Platelet Derived Growth Factor-BB Recombinant Protein | ePDGFBB recombinant protein
Recombinant Equine Platelet Derived Growth Factor-BB
Purity
Greater than 95.0% as determined by SDS-PAGE.
Synonyms
Equine Platelet Derived Growth Factor-BB; Recombinant Equine Platelet Derived Growth Factor-BB; Platelet Derived Growth Factor-BB Equine Recombinant; Glioma-derived growth factor; GDGF; Osteosarcoma-derived Growth Factor; ODGF; SIS; SSV; PDGF2; c-sis; FLJ12858; PDGF-BB; PDGF B-chain; Platelet-derived growth factor beta polypeptide; Becaplermin; PDGF-BB Equine; ePDGFBB recombinant protein
Host
E Coli
Purity/Purification
Greater than 95.0% as determined by SDS-PAGE.
Form/Format
Lyophilized from a sterile (0.2u) filtered solution containing 10 mM sodium phosphate, pH7.5.
Sterile Filtered White lyophilized (freeze-dried) powder.
Sterile Filtered White lyophilized (freeze-dried) powder.
Sequence
MSLGSLAVAE PAMIAECKTR TEVFEISRRL IDRTNANFLV WPPCVEVQRC SGCCNNRHVQ CRPTQVQLRP VQVRKIEIVR KKPTFKKATV TLEDHLACKC ETVGAARPVT
Reconstitution
It is recommended to reconstitute the lyophilized PDGF-BB in sterile water at a concentration of 0.1 mg/ml, which can then be further diluted to other aqueous solutions.
Biological Activity
The ED50 as determined by 3T3 proliferation is <= 30ng/ml, corresponding to a specific activity of >= 3.3 x 10^4 units/mg.
Preparation and Storage
Lyophilized PDGF-BB although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution PDGF-BB Human should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Related Product Information for ePDGFBB recombinant protein
Introduction: PDGF-BB is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 7, at sites where this gene and that for COL1A1 are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans resulting from unregulated expression of growth factor. Two splice variants have been identified for this gene.
Description: PDGF-BB Equine Recombinant produced in E Coli is a single, non-glycosylated, polypeptide dimer chain containing 2 x 110 amino acids and having a total molecular mass of 24.8kDa.
The PDGF-BB is purified by proprietary chromatographic techniques.
Description: PDGF-BB Equine Recombinant produced in E Coli is a single, non-glycosylated, polypeptide dimer chain containing 2 x 110 amino acids and having a total molecular mass of 24.8kDa.
The PDGF-BB is purified by proprietary chromatographic techniques.
Product Categories/Family for ePDGFBB recombinant protein
NCBI and Uniprot Product Information
Similar Products
Product Notes
The ePDGFBB (Catalog #AAA147339) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: MSLGSLAVAE PAMIAECKTR TEVFEISRRL IDRTNANFLV WPPCVEVQRC SGCCNNRHVQ CRPTQVQLRP VQVRKIEIVR KKPTFKKATV TLEDHLACKC ETVGAARPVT. It is sometimes possible for the material contained within the vial of "Equine Platelet Derived Growth Factor-BB, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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