Cyclophilin-H Recombinant Protein | PPIH recombinant protein

Recombinant Human Cyclophilin-H, His Tag

Cat. Num. AAA146277

• Gene Names: PPIH; CYPH; CYP-20; USA-CYP; SnuCyp-20
• Purity: Purity as determined by densitometric image analysis is greater than 95%.
• Synonyms: Cyclophilin-H; Recombinant Human Cyclophilin-H; His Tag; PPIH Human; His; Cyclophilin-H Human Recombinant; Oeptidylprolyl Isomerase H; PPIH; CYPH; CYP20; SnuCyp-20; Peptidyl-prolyl cis-trans isomerase H; PPIase H; Rotamase H; U-snRNP-associated cyclophilin SnuCyp-20; USA-CYP; Small nuclear ribonucleoprotein particle-specific cyclophilin H; peptidylprolyl isomerase H; CYP-20; MGC5016; PPIH His; PPIH recombinant protein

• Host: E Coli
• Purity/Purification: Purity as determined by densitometric image analysis is greater than 95%.
• Form/Format: Filtered (0.4 um) and lyophilized from 0.5 mg/ml in phosphate buffered saline pH 7.4.; Filtered White lyophilized (freeze-dried) powder.
• Sequence: MKHHHHHHASAVANSSPVNP VVFFDVSIGG QEVGRMKIEL FADVVPKTAE NFRQFCTGEF RKDGVPIGYK GSTFHRVIKD FMIQGGDFVN GDGTGVASIY RGPFADENFK LRHSAPGLLS MANSGPSTNG CQFFITCSKC DWLDGKHVVF GKIIDGLLVM RKIENVPTGP NNKPKLPVVI SQCGEM
• Sequence Length: 177

• Solubility: It is recommended to add 200 ul deionized water to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. PPIH is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
• Preparation and Storage: Store lyophilized protein at -20 degree C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4 degree C for a limited period of time; it does not show any change after two weeks at 4 degree C.

Related Product Information for PPIH recombinant protein

Description: PPIH Human Recombinant produced in E Coli is a single, non-glycosylated, polypeptide chain (a.a 2-177) containing 186 amino acids and including a 10 a.a N-terminal His tag. The total molecular mass is 20.3kDa (calculated).

Introduction: PPIH is a part of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and increase protein folding. PPIH enzyme is a precise factor of the complex that comprises pre-mRNA processing factors PRPF3, PRPF4, and PRPF18, as well as U4/U5/U6 tri-snRNP. PPIH possess PPIase activity and acts as a protein chaperone that mediates the interactions between different proteins inside the spliceosome.

Product Categories/Family for PPIH recombinant protein

Enzymes; Cyclophilin

NCBI and Uniprot Product Information

• NCBI GI #: 5454154
• NCBI GeneID: 10465
• NCBI Accession #: NP_006338.1
• NCBI GenBank Nucleotide #: NM_006347.3
• UniProt Accession #: O43447; A6NNE7
• Molecular Weight: 14,492 Da
• NCBI Official Full Name: peptidyl-prolyl cis-trans isomerase H
• NCBI Official Synonym Full Names: peptidylprolyl isomerase H (cyclophilin H)
• NCBI Official Symbol: PPIH
• NCBI Official Synonym Symbols: CYPH; CYP-20; USA-CYP; SnuCyp-20
• NCBI Protein Information: peptidyl-prolyl cis-trans isomerase H; PPIase h; U-snRNP-associated cyclophilin SnuCyp-20; U-snRNP-associated cyclophilin SunCyp-20; USA-CyP SnuCyp-20; cyclophilin H; rotamase H; small nuclear ribonucleoprotein particle-specific cyclophilin H
• UniProt Protein Name: Peptidyl-prolyl cis-trans isomerase H
• UniProt Gene Name: PPIH
• UniProt Synonym Gene Names: CYP20; CYPH; PPIase H; CypH; USA-CYP
• UniProt Entry Name: PPIH_HUMAN

NCBI Description

The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein is a specific component of the complex that includes pre-mRNA processing factors PRPF3, PRPF4, and PRPF18, as well as U4/U5/U6 tri-snRNP. This protein has been shown to possess PPIase activity and may act as a protein chaperone that mediates the interactions between different proteins inside the spliceosome. [provided by RefSeq, Jul 2008]

Uniprot Description

PPIH: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Participates in pre-mRNA splicing. May play a role in the assembly of the U4/U5/U6 tri-snRNP complex. May act as a chaperone. Belongs to the cyclophilin-type PPIase family. PPIase H subfamily.

Protein type: Spliceosome; RNA processing; EC 5.2.1.8; Isomerase; Cyclophilin

Chromosomal Location of Human Ortholog: 1p34.1

Cellular Component: spliceosome; U4/U6 x U5 tri-snRNP complex; cytoplasm; nuclear speck

Molecular Function: ribonucleoprotein binding; protein binding; peptidyl-prolyl cis-trans isomerase activity; cyclosporin A binding

Biological Process: nuclear mRNA splicing, via spliceosome; protein peptidyl-prolyl isomerization; positive regulation of viral genome replication; protein folding; protein complex assembly