SET recombinant protein

Recombinant Human SET

Cat. Num. AAA145876

• Gene Names: SET; 2PP2A; IGAAD; TAF-I; I2PP2A; IPP2A2; PHAPII; TAF-IBETA
• Purity: Greater than 90% as determined by SDS-PAGE.
• Synonyms: SET; Recombinant Human SET; SET Human; SET Human Recombinant; 2PP2A; I2PP2A; IGAAD; IPP2A2; PHAPII; TAF-I; TAF-IBETA; Protein SET; HLA-DR-associated protein II; Inhibitor of granzyme A-activated DNase; Template-activating factor I; SET recombinant protein

• Host: E Coli
• Purity/Purification: Greater than 90% as determined by SDS-PAGE.
• Form/Format: The SET solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 20% glycerol and 1mM DTT.; Sterile Filtered clear solution.
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAPKRQS PLPPQKKKPR PPPALGPEET SASAGLPKKG EKEQQEAIEH IDEVQNEIDR LNEQASEEIL KVEQKYNKLR QPFFQKRSEL IAKIPNFWVT TFVNHPQVSA LLGEEDEEAL HYLTRVEVTE FEDIKSGYRI DFYFDENPYF ENKVLSKEFH LNESGDPSSK STEIKWKSGK DLTKRSSQTQ NKASRKRQHE EPESFFTWFT DHSDAGADEL GEVIKDDIWP NPLQYYLVPD MDDEEGEGEE DDDDDEEEEG LEDIDEEGDE DEGEEDEDDD EGEEGEEDEG EDD
• Sequence Length: 290

Related Product Information for SET recombinant protein

Description: SET Human Recombinant produced in E Coli is a single polypeptide chain containing 313 amino acids (1-290) and having a molecular mass of 35.9kDa. SET is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Introduction: SET is part of a complex localized to the endoplasmic reticulum however is found in the nucleus. SET inhibits apoptosis following attack by cytotoxic T lymphocytes and can also enhance DNA replication of the adenovirus genome. SET inhibits acetylation of nucleosomes, particularly histone H4, by histone acetylases (HAT). This inhibition is accomplished by masking histone lysines from being acetylated.

Product Categories/Family for SET recombinant protein

Recombinant & Natural Proteins; Recombinant Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 170763500
• NCBI GeneID: 6418
• NCBI Accession #: NP_001116293.1
• NCBI GenBank Nucleotide #: NM_001122821.1
• UniProt Accession #: Q01105; Q15541; Q5VXV1; Q5VXV2; Q6FHZ5; A5A5H4; A6NGV1; B4DUE2
• Molecular Weight: 31,272 Da
• NCBI Official Full Name: protein SET isoform 1
• NCBI Official Synonym Full Names: SET nuclear proto-oncogene
• NCBI Official Symbol: SET
• NCBI Official Synonym Symbols: 2PP2A; IGAAD; TAF-I; I2PP2A; IPP2A2; PHAPII; TAF-IBETA
• NCBI Protein Information: protein SET; HLA-DR-associated protein II; SET nuclear oncogene; SET translocation (myeloid leukemia-associated); Template-Activating Factor-I, chromatin remodelling factor; inhibitor of granzyme A-activated DNase; inhibitor-2 of protein phosphatase-2A; phosphatase 2A inhibitor I2PP2A; protein phosphatase type 2A inhibitor
• UniProt Protein Name: Protein SET
• Protein Family: Set3 complex
• UniProt Gene Name: SET
• UniProt Synonym Gene Names: IGAAD; I-2PP2A; TAF-I
• UniProt Entry Name: SET_HUMAN

NCBI Description

The protein encoded by this gene inhibits acetylation of nucleosomes, especially histone H4, by histone acetylases (HAT). This inhibition is most likely accomplished by masking histone lysines from being acetylated, and the consequence is to silence HAT-dependent transcription. The encoded protein is part of a complex localized to the endoplasmic reticulum but is found in the nucleus and inhibits apoptosis following attack by cytotoxic T lymphocytes. This protein can also enhance DNA replication of the adenovirus genome. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

Uniprot Description

SET: a potent inhibitor of protein phosphatase 2A involved in apoptosis, transcription, nucleosome assembly and histone binding. The translocation t(6;9)(q21;q34.1) NUP214/CAN is found in some cases of acute undifferentiated leukemia (AUL). Two alternatively spliced isoforms have been described. Isoform 1 and isoform 2 interact directly with each other and with ANP32A within the tripartite INHAT (inhibitor of acetyltransferases) complex. Both isoforms inhibit the acetylation of histones (HAT) and nucleosomes, most probably by masking the accessibility of lysines of histones to the acetylases. The predominant target for inhibition is histone H4. HAT inhibition leads to silencing of HAT-dependent transcription and prevents active demethylation of DNA. Both isoforms stimulate DNA replication of the adenovirus genome complexed with viral core proteins; however, isoform 2 specific activity is higher. Isoform 2 anti-apoptotic activity is mediated by inhibition of the GZMA-activated DNase, NME1. In the course of cytotoxic T lymphocyte (CTL)-induced apoptosis, GZMA cleaves SET, disrupting its binding to NME1 and releasing NME1 inhibition.

Protein type: Nuclear receptor co-regulator; DNA-binding; Apoptosis; DNA replication; Oncoprotein; Inhibitor

Chromosomal Location of Human Ortholog: 9q34

Cellular Component: nucleoplasm; protein complex; perinuclear region of cytoplasm; endoplasmic reticulum; cytoplasm; cytosol; nucleus

Molecular Function: protein binding; DNA binding; histone binding; protein phosphatase type 2A regulator activity; protein phosphatase inhibitor activity

Biological Process: nucleosome assembly; nucleosome disassembly; regulation of catalytic activity; negative regulation of catalytic activity; nucleocytoplasmic transport; gene expression; negative regulation of neuron apoptosis; mitotic cell cycle; DNA replication; negative regulation of transcription, DNA-dependent; negative regulation of histone acetylation