Dihydrofolate Reductase Active Protein | mDHFR active protein

Recombinant Mouse Dihydrofolate Reductase

Cat. Num. AAA144363

• Gene Names: Dhfr; AA607882; AI662710; AW555094; 8430436I03Rik
• Purity: Greater than 95.0% as determined by SDS-PAGE.
• Synonyms: Dihydrofolate Reductase; Recombinant Mouse Dihydrofolate Reductase; DHFR Mouse; Dihydrofolate Reductase Mouse Recombinant; Dihydrofolate reductase; DHFR; DHFRP1; AA607882; AI662710; AW555094; 8430436I03Rik; mDHFR active protein

• Host: E Coli
• Purity/Purification: Greater than 95.0% as determined by SDS-PAGE.
• Form/Format: The DHFR solution (1 mg/ml) contains 20mM Tris-HCl buffer(pH 8.0), 10% glycerol, 2mM DTT and 0.1M NaCl.; Sterile Filtered colorless solution.
• Sequence: MGSSHHHHHH SSGLVPRGSH MVRPLNCIVA VSQNMGIGKN GDLPWPPLRN EFKYFQRMTT TSSVEGKQNL VIMGRKTWFS IPEKNRPLKD RINIVLSREL KEPPRGAHFL AKSLDDALRL IEQPELASKV DMVWIVGGSS VYQEAMNQPG HLRLFVTRIM QEFESDTFFP EIDLGKYKLL PEYPGVLSEV QEEKGIKYKF EVYEKKD
• Sequence Length: 187

• Biological Activity: Specific activity is > 0.2 units/mg, in which one unit will convert 1.0 umole of 7,8 dihydrofloate and beta-NADPH to 5,6,7,8-tetrahydrofloate and beta-NADP per min at pH 6.5 at 25C.
• Preparation and Storage: Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Related Product Information for mDHFR active protein

Description: DHFR Mouse Recombinant fused with a 20 amino acid His tag at N-terminus produced in E Coli is a single, non-glycosylated, polypeptide chain containing 207 amino acids (1-187 a.a.) and having a molecular mass of 23.8kDa. The DHFR is purified by proprietary chromatographic techniques.

Introduction: Dihydrofolate reductase (DHFR) is an enzyme that reduces dihydrofolic acid to tetrahydrofolic acid, with NADPH as electron donor that can be converted to the kinds of tetrahydrofolate cofactors applied in 1-carbon transfer chemistry. DHFR converts dihydrofolate into tetrahydrofolate, which is a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and specific amino acids. Even though the functional DHFR gene is mapped to chromosome 5, numerous intronless processed pseudogenes or dihydrofolate reductase-like genes are identified on separate chromosomes. DHFR deficiency is associated with megaloblastic anemia. DHFR knockdown plays a role in the anticancer activity of 2-hydroxyoleic acid. DHFR gene insertion/deletion polymorphism is linked to variation in serum and red blood cell folate concentrations in women.

Product Categories/Family for mDHFR active protein

Enzymes; Reductase

NCBI and Uniprot Product Information

• NCBI GI #: 7106289
• NCBI GeneID: 13361
• NCBI Accession #: NP_034179.1
• NCBI GenBank Nucleotide #: NM_010049.3
• UniProt Accession #: P00375; P70693; Q61485; Q61487; Q61579
• Molecular Weight: 21,606 Da
• NCBI Official Full Name: dihydrofolate reductase
• NCBI Official Synonym Full Names: dihydrofolate reductase
• NCBI Official Symbol: Dhfr
• NCBI Official Synonym Symbols: AA607882; AI662710; AW555094; 8430436I03Rik
• NCBI Protein Information: dihydrofolate reductase
• UniProt Protein Name: Dihydrofolate reductase
• Protein Family: Dihydrofolate reductase
• UniProt Gene Name: Dhfr
• UniProt Entry Name: DYR_MOUSE

Uniprot Description

DHFR: Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFRL1. Defects in DHFR are the cause of megaloblastic anemia due to dihydrofolate reductase deficiency (DHFRD). DHFRD is an inborn error of metabolism, characterized by megaloblastic anemia and/or pancytopenia, severe cerebral folate deficiency, and cerebral tetrahydrobiopterin deficiency. Clinical features include variable neurologic symptoms, ranging from severe developmental delay and generalized seizures in infancy, to childhood absence epilepsy with learning difficulties, to lack of symptoms. Belongs to the dihydrofolate reductase family.

Protein type: Cofactor and Vitamin Metabolism - folate biosynthesis; Oxidoreductase; Cofactor and Vitamin Metabolism - one carbon pool by folate; EC 1.5.1.3

Molecular Function: mRNA binding; dihydrofolic acid binding; RNA binding; oxidoreductase activity; drug binding; dihydrofolate reductase activity; NADP binding

Biological Process: tetrahydrofolate metabolic process; tetrahydrofolate biosynthetic process; nucleotide biosynthetic process; dihydrofolate metabolic process; one-carbon compound metabolic process; response to methotrexate; glycine biosynthetic process