Keratinocyte Growth Factor Active Protein | mKGF active protein

Recombinant Mouse Keratinocyte Growth Factor

Cat. Num. AAA144085

• Gene Names: Fgf7; Kgf
• Purity: Greater than 95.0% as determined by: (a) Analysis by HPLC. (b) Analysis by SDS-PAGE.
• Synonyms: Keratinocyte Growth Factor; Recombinant Mouse Keratinocyte Growth Factor; KGF Mouse; Keratinocye Growth Factor Mouse Recombinant; HBGF-7; FGF7; FGF-7; KGF; Keratinocyte growth factor; Fibroblast growth factor 7; Heparin-binding growth factor 7; mKGF active protein

• Host: E Coli
• Purity/Purification: Greater than 95.0% as determined by: (a) Analysis by HPLC. (b) Analysis by SDS-PAGE.
• Form/Format: The protein was lyophilized from a concentrated (1mg/ml) solution containing 20mM Phosphate buffer pH-8 and 0.1M NaCl.; Sterile Filtered White lyophilized (freeze-dried) powder.
• Sequence: MCNDMSPEQT ATSVNCSSPE RHTRSYDYME GGDIRVRRLF CRTQWYLRID KRGKVKGTQE MKNSYNIMEI RTVAVGIVAI KGVESEYYLA MNKEGKLYAK KECNEDCNFK ELILENHYNT YASAKWTHSG GEMFVALNQK GIPVKGKKTK KEQKTAHFLP MAIT
• Sequence Length: 194

• Solubility: It is recommended to reconstitute the lyophilized KGF in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
• Biological Activity: The ED50, calculated by the dose-dependant stimulation of KGF-responsive BaF3 indicator cells (measured by 3H-thymidine uptake) is < 10ng/ml corresponding to a specific activity of 100,000 Units/mg.
• Preparation and Storage: Lyophilized KGF although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution FGF7 should be stored at 4 degree C between 2-7 days and for future use below -18 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Related Product Information for mKGF active protein

Description: Keratinocyte Growth Factor-1 Mouse Recombinant produced in E Coli is a single, non-glycosylated, polypeptide chain containing 164 amino acids and having a molecular mass of 18.9 kDa. The FGF-7 is purified by proprietary chromatographic techniques.

Introduction: KGF is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF7 is a potent epithelial cell-specific growth factor, whose mitogenic activity is predominantly exhibited in keratinocytes but not in fibroblasts and endothelial cells. Studies of mouse and rat homologs of this gene implicated roles in morphogenesis of epithelium, reepithelialization of wounds, hair development and early lung organogenesis.

Product Categories/Family for mKGF active protein

Cytokines and Growth Factors; Growth Factors; Keratinocyte Growth Factor

NCBI and Uniprot Product Information

• NCBI GI #: 6679785
• NCBI GeneID: 14178
• NCBI Accession #: NP_032034.1
• NCBI GenBank Nucleotide #: NM_008008.4
• UniProt Accession #: P36363
• Molecular Weight: 22,347 Da
• NCBI Official Full Name: fibroblast growth factor 7
• NCBI Official Synonym Full Names: fibroblast growth factor 7
• NCBI Official Symbol: Fgf7
• NCBI Official Synonym Symbols: Kgf
• NCBI Protein Information: fibroblast growth factor 7; FGF-7; HBGF-7; Keratinocyte growth factor; heparin-binding growth factor 7
• UniProt Protein Name: Fibroblast growth factor 7
• UniProt Gene Name: Fgf7
• UniProt Synonym Gene Names: Fgf-7; Kgf; FGF-7; HBGF-7; KGF
• UniProt Entry Name: FGF7_MOUSE

Uniprot Description

FGF7: Plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. Required for normal branching morphogenesis. Growth factor active on keratinocytes. Possible major paracrine effector of normal epithelial cell proliferation. Belongs to the heparin-binding growth factors family.

Protein type: Cytokine; Secreted, signal peptide; Secreted

Cellular Component: Golgi apparatus; extracellular space; cell; extracellular region

Molecular Function: heparin binding; protein binding; growth factor activity; type 2 fibroblast growth factor receptor binding; receptor binding; fibroblast growth factor receptor binding; chemoattractant activity

Biological Process: positive regulation of keratinocyte migration; fibroblast growth factor receptor signaling pathway; hair follicle morphogenesis; wound healing; mesenchymal cell proliferation; phospholipid biosynthetic process; positive regulation of peptidyl-tyrosine phosphorylation; positive chemotaxis; ovarian follicle development; positive regulation of cell division; positive regulation of cell proliferation; actin cytoskeleton reorganization; phosphatidylcholine biosynthetic process; surfactant homeostasis; positive regulation of epithelial cell proliferation; lung development