Histone Acetyltransferase 1 Recombinant Protein | HAT1 recombinant protein

Recombinant Human Histone Acetyltransferase 1

Cat. Num. AAA144000

• Gene Names: HAT1; KAT1
• Purity: Greater than 90% as determined by SDS-PAGE.
• Synonyms: Histone Acetyltransferase 1; Recombinant Human Histone Acetyltransferase 1; HAT1 Human; Histone Acetyltransferase 1 Human Recombinant; EC 2.3.1.48; KAT1; histone acety ltransferase 1; Histone acetyltransferase type B catalytic subunit; HAT1; HAT1 recombinant protein

• Host: E Coli
• Purity/Purification: Greater than 90% as determined by SDS-PAGE.
• Form/Format: The HAT1 solution contains 20mM Tris-HCl pH-8, 1mM DTT, and 10% glycerol.; Sterile Filtered clear colorless solution.
• Sequence: MGSSHHHHHH SSGLVPRGSH MKKLAEYKCN TNTAIELKLV RFPEDLENDI RTFFPEYTHQ LFGDDETAFG YKGLKILLYY IAGSLSTMFR VEYASKVDEN FDCVEADDVE GKIRQIIPPG FCTNTNDFLS LLEKEVDFKP FGTLLHTYSV LSPTGGENFT FQIYKADMTC RGFREYHERL QTFLMWFIET ASFIDVDDER WHYFLVFEKY NKDGATLFAT VGYMTVYNYY VYPDKTRPRV SQMLILTPFQ GQGHGAQLLE TVHRYYTEFP TVLDITAEDP SKSYVKLRDF VLVKLCQDLP CFSREKLMQG FNEDMAIEAQ QKFKINKQHA RRVYEILRLL VTD
• Sequence Length: 419

• Preparation and Storage: HAT1 Recombinant Human although stable at 4 degree C for 30 days, should be stored below -20 degree C for periods greater than 30 days. Please avoid freeze-thaw cycles.

Related Product Information for HAT1 recombinant protein

Description: HAT1 Human Recombinant produced in E Coli is a single, non-glycosylated, polypeptide chain containing 343 amino acids (20-341 a.a.) and having a molecular mass of 40.1 kDa. The HAT1 is fused to a 21 amino acid His Tag and purified by proprietary chromatographic techniques.

Introduction: HAT1 is a type B histone acetyltransferase that participates in the rapid acetylation of newly synthesized cytoplasmic histones, which are in turn imported into the nucleus for de novo deposition onto nascent DNA chains. Histone acetylation, mainly of soluble histone H4 at ''Lys-5'' and ''Lys-12'' and acetylates histone H2A at ''Lys-5''. HAT1 takes part in replication-dependent chromatin assembly. HAT1 acetylates soluble but not nucleosomal histone H4 at lysines 5 and 12, and to a lesser degree, histone H2A at lysine 5. HAT1 has intrinsic substrate specificity that modifies lysine in recognition sequence GXGKXG.

Product Categories/Family for HAT1 recombinant protein

Enzymes; Transferase

NCBI and Uniprot Product Information

• NCBI GI #: 4504341
• NCBI GeneID: 8520
• NCBI Accession #: NP_003633.1
• NCBI GenBank Nucleotide #: NM_003642.3
• UniProt Accession #: O14929; Q49A44; Q53QF0; Q53SU4; Q6P594; Q8WWB9
• Molecular Weight: 39,786 Da
• NCBI Official Full Name: histone acetyltransferase type B catalytic subunit
• NCBI Official Synonym Full Names: histone acetyltransferase 1
• NCBI Official Symbol: HAT1
• NCBI Official Synonym Symbols: KAT1
• NCBI Protein Information: histone acetyltransferase type B catalytic subunit
• UniProt Protein Name: Histone acetyltransferase type B catalytic subunit
• Protein Family: Histone acetyltransferase
• UniProt Gene Name: HAT1
• UniProt Synonym Gene Names: KAT1
• UniProt Entry Name: HAT1_HUMAN

NCBI Description

The protein encoded by this gene is a type B histone acetyltransferase (HAT) that is involved in the rapid acetylation of newly synthesized cytoplasmic histones, which are in turn imported into the nucleus for de novo deposition onto nascent DNA chains. Histone acetylation, particularly of histone H4, plays an important role in replication-dependent chromatin assembly. Specifically, this HAT can acetylate soluble but not nucleosomal histone H4 at lysines 5 and 12, and to a lesser degree, histone H2A at lysine 5. Alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Jun 2009]

Uniprot Description

HAT1: Acetylates soluble but not nucleosomal histone H4 at 'Lys-5' (H4K5ac) and 'Lys-12' (H4K12ac) and, to a lesser extent, acetylates histone H2A at 'Lys-5' (H2AK5ac). Has intrinsic substrate specificity that modifies lysine in recognition sequence GXGKXG. May be involved in nucleosome assembly during DNA replication and repair as part of the histone H3.1 and H3.3 complexes. May play a role in DNA repair in response to free radical damage. Belongs to the HAT1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.3.1.48; Acetyltransferase

Chromosomal Location of Human Ortholog: 2q31.2-q33.1

Cellular Component: nucleoplasm; nuclear matrix; protein complex; intracellular membrane-bound organelle; nuclear chromatin; cytoplasm; nucleus

Molecular Function: protein binding; H4 histone acetyltransferase activity; histone acetyltransferase activity

Biological Process: establishment and/or maintenance of chromatin architecture; DNA replication-independent nucleosome assembly; internal protein amino acid acetylation; DNA packaging; DNA replication-dependent nucleosome assembly; response to nutrient; chromatin silencing at telomere