Phenylethanolamine-N-Methyltransferase Recombinant Protein | PNMT recombinant protein
Recombinant Human Phenylethanolamine-N-Methyltransferase
Gene Names
PNMT; PENT; PNMTase
Purity
Greater than 95.0% as determined by SDS-PAGE.
Synonyms
Phenylethanolamine-N-Methyltransferase; Recombinant Human Phenylethanolamine-N-Methyltransferase; PNMT Human; Phenylethanolamine-N-Methyltransferase Human Recombinant; PENT; PNMTase; Noradrenaline-N-methyltransferase; Phenylethanolamine N-methyltransferase; PNMT; MGC34570; PNMT recombinant protein
Host
E Coli
Purity/Purification
Greater than 95.0% as determined by SDS-PAGE.
Form/Format
The PNMT protein solution contains 20mM Tris-HCl, pH-8 & 10% Glycerol.
Sterile filtered colorless solution.
Sterile filtered colorless solution.
Sequence
MSGADRSPNA GAAPDSAPGQ AAVASAYQRF EPRAYLRNNY APPRGDLCNP NGVGPWKLRC LAQTFATGEV SGRTLIDIGS GPTVYQLLSA CSHFEDITMT DFLEVNRQEL GRWLQEEPGA FNWSMYSQHA CLIEGKGECW QDKERQLRAR VKRVLPIDVH QPQPLGAGSP APLPADALVS AFCLEAVSPD LASFQRALDH ITTLLRPGGH LLLIGALEES WYLAGEARLT VVPVSEEEVR EALVRSGYKV RDLRTYIMPA HLQTGVDDVK GVFFAWAQKV GL
Sequence Length
282
Preparation and Storage
Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Related Product Information for PNMT recombinant protein
Description: Recombinant Human PNMT produced in E Coli is a single, non-glycosylated polypeptide chain containing 282 amino acids (1-282 a.a.) and having a molecular mass of 30.8 kDa.PNMT is purified by conventional chromatographic techniques.
Introduction: PNMT is an enzyme located in the adrenal medulla and it catalyzes the final step of the catecholamine biosynthesis pathway, which methylates norepinephrine to form epinephrine (adrenaline). PNMT has beta-carboline 2N-methyltransferase activity. PNMT takes part in regulating epinephrine production. Glucocorticoid receptors form multimers of PNMT independent of the DNA binding domain. PNMT expression is regulated late in mouse gestation by AP2-alpha and glucocorticoids.
Introduction: PNMT is an enzyme located in the adrenal medulla and it catalyzes the final step of the catecholamine biosynthesis pathway, which methylates norepinephrine to form epinephrine (adrenaline). PNMT has beta-carboline 2N-methyltransferase activity. PNMT takes part in regulating epinephrine production. Glucocorticoid receptors form multimers of PNMT independent of the DNA binding domain. PNMT expression is regulated late in mouse gestation by AP2-alpha and glucocorticoids.
Product Categories/Family for PNMT recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
30,855 Da
NCBI Official Full Name
phenylethanolamine N-methyltransferase
NCBI Official Synonym Full Names
phenylethanolamine N-methyltransferase
NCBI Official Symbol
PNMT
NCBI Official Synonym Symbols
PENT; PNMTase
NCBI Protein Information
phenylethanolamine N-methyltransferase; noradrenaline N-methyltransferase; phenylethanolamine N-methylase
UniProt Protein Name
Phenylethanolamine N-methyltransferase
Protein Family
UniProt Gene Name
PNMT
UniProt Synonym Gene Names
PENT; PNMTase
UniProt Entry Name
PNMT_HUMAN
NCBI Description
The product of this gene catalyzes the last step of the catecholamine biosynthesis pathway, which methylates norepinephrine to form epinephrine (adrenaline). The enzyme also has beta-carboline 2N-methyltransferase activity. This gene is thought to play a key step in regulating epinephrine production. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Nov 2012]
Uniprot Description
PNMT: Converts noradrenaline to adrenaline. Belongs to the NNMT/PNMT/TEMT family.
Protein type: Amino Acid Metabolism - tyrosine; Methyltransferase; EC 2.1.1.28
Chromosomal Location of Human Ortholog: 17q12
Cellular Component: cytosol
Molecular Function: phenylethanolamine N-methyltransferase activity
Biological Process: methylation; catecholamine biosynthetic process; epinephrine biosynthetic process
Disease: Hypertension, Essential
Research Articles on PNMT
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Product Notes
The PNMT pnmt (Catalog #AAA143536) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: MSGADRSPNA GAAPDSAPGQ AAVASAYQRF EPRAYLRNNY APPRGDLCNP NGVGPWKLRC LAQTFATGEV SGRTLIDIGS GPTVYQLLSA CSHFEDITMT DFLEVNRQEL GRWLQEEPGA FNWSMYSQHA CLIEGKGECW QDKERQLRAR VKRVLPIDVH QPQPLGAGSP APLPADALVS AFCLEAVSPD LASFQRALDH ITTLLRPGGH LLLIGALEES WYLAGEARLT VVPVSEEEVR EALVRSGYKV RDLRTYIMPA HLQTGVDDVK GVFFAWAQKV GL. It is sometimes possible for the material contained within the vial of "Phenylethanolamine-N-Methyltransferase, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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