Cytokeratin 19 Recombinant Protein | KRT19 recombinant protein

Recombinant Human Cytokeratin 19

Cat. Num. AAA142905

• Gene Names: KRT19; K19; CK19; K1CS
• Synonyms: Cytokeratin 19; Recombinant Human Cytokeratin 19; KRT19 Human; Cytokeratin 19 Human Recombinant; Keratin type I cytoskeletal 19; Cytokeratin-19; CK-19; Keratin-19; K19; KRT19; CK19; K1CS; MGC15366; KRT19 recombinant protein

• Host: E Coli
• Form/Format: The protein (1mg/ml) was lyophilized after from a sterile solution containing 30mM Tris-HCl pH-8, 9.5M urea, 2mM DTT, 2mM EDTA and 10mM methylammonium chloride.; Sterile Filtered White lyophilized (freeze-dried) powder.
• Sequence Length: 400

• Solubility: It is recommended to reconstitute the lyophilized KRT19 in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
• Reconstitution: Performed by mixing equimolar amounts of cytokeratins of type I and type II at concentrations of approx. 0.5 mg/ml, both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl, 2 mM dithiothreitol, 10 mM Tris-HCl, pH 7.4). For immunization purposes, the solution can be further dialyzed against PBS (phosphate buffered saline, e.g. Dulbecco's PBS).
• Preparation and Storage: Lyophilized KRT19 although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution KRT19 should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Related Product Information for KRT19 recombinant protein

Description: Cytokeratin 19 Human Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain having a molecular mass of 44,098 Dalton. The KRT19 is purified by proprietary chromatographic techniques.

Introduction: CTK-19 is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. The type I cytokeratins are clustered in a region of chromosome 17q12-q21.

Product Categories/Family for KRT19 recombinant protein

Recombinant & Natural Proteins; Recombinant Proteins; Cytokeratin

NCBI and Uniprot Product Information

• NCBI GI #: 24234699
• NCBI GeneID: 3880
• NCBI Accession #: NP_002267.2
• NCBI GenBank Nucleotide #: NM_002276.4
• UniProt Accession #: P08727; Q5XG83; Q6NW33; Q7L5M9; Q96A53; Q96FV1; Q9BYF9; Q9P1Y4; B2R874
• Molecular Weight: 44,106 Da
• NCBI Official Full Name: keratin, type I cytoskeletal 19
• NCBI Official Synonym Full Names: keratin 19, type I
• NCBI Official Symbol: KRT19
• NCBI Official Synonym Symbols: K19; CK19; K1CS
• NCBI Protein Information: keratin, type I cytoskeletal 19; 40-kDa keratin intermediate filament; CK-19; cytokeratin 19; cytokeratin-19; keratin, type I, 40-kd
• UniProt Protein Name: Keratin, type I cytoskeletal 19
• Protein Family: Keratin
• UniProt Gene Name: KRT19
• UniProt Synonym Gene Names: CK-19; K19
• UniProt Entry Name: K1C19_HUMAN

NCBI Description

The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. The type I cytokeratins are clustered in a region of chromosome 17q12-q21. [provided by RefSeq, Jul 2008]

Uniprot Description

K19: a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Keratin 19 differs from all other IF proteins in lacking the C-terminal tail domain. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 17q21.2

Cellular Component: dystrophin-associated glycoprotein complex; costamere; intermediate filament; Z disc; sarcolemma

Molecular Function: protein binding; structural constituent of cytoskeleton; structural constituent of muscle; protein complex binding

Biological Process: viral reproduction; response to estrogen stimulus; sarcomere organization