Probable RNA helicase SDE3 (SDE3) Recombinant Protein | SDE3 recombinant protein

Recombinant Arabidopsis thaliana Probable RNA helicase SDE3 (SDE3) , partial

• Gene Names: SDE3; SILENCING DEFECTIVE; T25N20.11; T25N20_11
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Probable RNA helicase SDE3 (SDE3); Recombinant Arabidopsis thaliana Probable RNA helicase SDE3 (SDE3); partial; SDE3 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Length: 1,002

• Species: Arabidopsis thaliana (Mouse-ear cress)
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

NCBI and Uniprot Product Information

• NCBI GI #: 15220521
• NCBI GeneID: 837047
• NCBI Accession #: NP_172037.1
• NCBI GenBank Nucleotide #: NM_100425.4
• UniProt Accession #: Q8GYD9; Q94KS1; Q9LR48
• Molecular Weight: 113,363 Da
• NCBI Official Full Name: P-loop containing nucleoside triphosphate hydrolases superfamily protein
• NCBI Official Symbol: SDE3
• NCBI Official Synonym Symbols: SILENCING DEFECTIVE; T25N20.11; T25N20_11
• NCBI Protein Information: P-loop containing nucleoside triphosphate hydrolases superfamily protein
• UniProt Protein Name: Probable RNA helicase SDE3
• Protein Family: Probable RNA helicase
• UniProt Gene Name: SDE3

NCBI Description

Encodes a protein with similarity to RNA helicases. Mutants are defective in post-transcriptional gene silencing.

Uniprot Description

Probable RNA helicase required for post-transcriptional gene silencing (PTGS), a process that provides protection in plants against virus infection and can suppress expression of transgenes. Plays a central role in RNA interference (RNAi) process, a process that mediates mRNA destruction of translational repression. Required for the assembly of the RISC complex, a complex required for target RNA destruction or repression. May be required in the RISC assembly to unwind miRNAs, in the production of single-stranded miRNA from the double-stranded miRNA, a key step in RISC formation. Involved in the amplification of sense-PTGS (S-PTGS), leading to siRNA production. Required for the maintenance but not the initiation of tobacco rattle virus (TRV)-mediated silencing, probably by mediating/maintaining DNA methylation and chromatin-based transcriptional gene silencing at some genomic locations.