Interleukin-24 Active Protein | IL 24 active protein

Recombinant Human Interleukin-24

• Gene Names: IL24; C49A; FISP; MDA7; MOB5; ST16; IL10B
• Purity: Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Synonyms: Interleukin-24; Recombinant Human Interleukin-24; IL 24 Human; Interleukin-24 Human Recombinant; C49A; FISP; MDA7; ST16; IL-24; IL10B; Mob-5; MDA-7; Suppression of tumorigenicity 16 protein; Melanoma differentiation-associated gene 7 protein; IL 24 active protein

• Host: Sacharomyces cerevisiae
• Purity/Purification: Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 um filtered solution in PBS with BSA as a carrier.; Sterile Filtered White lyophilized (freeze-dried) powder.
• Sequence Length: 207

• Solubility: It is recommended to reconstitute the lyophilized IL-24 in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
• Biological Activity: Measured by its ability to bind to the cell receptor of Capan-1 cells line resulted in Stat-3 activation. The ED50 for this effect is typically 1.0 ng/ml, corresponding to a Specific Activity of 1x106 units/mg.
• Preparation and Storage: Lyophilized MDA7 although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution MDA-7 Recombinant should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Related Product Information for IL 24 active protein

Description: Interleukin 24 human recombinant produced in yeast is a single, glycosylated, polypeptide chain containing 158 amino acids and having a molecular mass of 18 kDa. As a result of glycosylation, the protein migrates at 19.5 kDa on SDS-PAGE.

Introduction: IL24 is a member of the IL10 family of cytokines. It was identified as a gene induced during terminal differentiation in melanoma cells. IL-10B encoded can induce apoptosis selectively in various cancer cells. Overexpression IL-24 leads to elevated expression of several GADD family genes, which correlates with the induction of apoptosis. The phosphorylation of mitogen-activated protein kinase 14 (MAPK7/P38), and heat shock 27kDa protein 1 (HSPB2/HSP27) are found to be induced by this gene in melanoma cells, but not in normal immortal melanocytes. Alternatively spliced transcript variants encoding distinct isoforms have been reported. The glycosylation is essential for activity of IL-24. Functionally, IL-24 has diverse activities. At low concentrations, it induces type I proinflammatory cytokines such as IFN-g, IL-1b, IL-12 and TNF-a. At high concentration, it is a strong inducer of apoptosis in tumor cells, but not normal cells. mda-7/IL-24 is being hailed as a ˜magic bullet' for cancer gene therapy.

Product Categories/Family for IL 24 active protein

Cytokines and Growth Factors; Cytokines; Interleukin

NCBI and Uniprot Product Information

• NCBI GI #: 297632401
• NCBI GeneID: 11009
• NCBI Accession #: NP_001172085.1
• NCBI GenBank Nucleotide #: NM_001185156.1
• UniProt Accession #: Q13007; Q2YHE5; Q53XZ7; Q5YLN8; Q96DB0; Q96KG4
• Molecular Weight: 7,347 Da
• NCBI Official Full Name: interleukin-24 isoform 3
• NCBI Official Synonym Full Names: interleukin 24
• NCBI Official Symbol: IL24
• NCBI Official Synonym Symbols: C49A; FISP; MDA7; MOB5; ST16; IL10B
• NCBI Protein Information: interleukin-24; IL-4-induced secreted protein; melanocyte-associated Mda-7; melanoma differentiation-associated gene 7 protein; suppression of tumorigenicity 16 (melanoma differentiation)
• UniProt Protein Name: Interleukin-24
• Protein Family: Interleukin
• UniProt Gene Name: IL24
• UniProt Synonym Gene Names: MDA7; ST16; IL-24; MDA-7
• UniProt Entry Name: IL24_HUMAN

NCBI Description

This gene encodes a member of the IL10 family of cytokines. It was identified as a gene induced during terminal differentiation in melanoma cells. The protein encoded by this gene can induce apoptosis selectively in various cancer cells. Overexpression of this gene leads to elevated expression of several GADD family genes, which correlates with the induction of apoptosis. The phosphorylation of mitogen-activated protein kinase 14 (MAPK7/P38), and heat shock 27kDa protein 1 (HSPB2/HSP27) are found to be induced by this gene in melanoma cells, but not in normal immortal melanocytes. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

IL24: Has antiproliferative properties on melanoma cells and may contribute to terminal cell differentiation. Belongs to the IL-10 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1q32

Cellular Component: extracellular space; endoplasmic reticulum

Molecular Function: cytokine activity

Biological Process: negative regulation of cell proliferation; wound healing; apoptosis; positive regulation of cell proliferation; positive regulation of JAK-STAT cascade; positive regulation of caspase activity; immune response; negative regulation of caspase activity; inflammatory response; negative regulation of cell migration; positive regulation of tyrosine phosphorylation of Stat3 protein; serine phosphorylation of STAT3 protein