Vascular Endothelial Growth Factor-C Active Protein | rVEGFC active protein

Recombinant Rat Vascular Endothelial Growth Factor-C (152)

Cat. Num. AAA142161

• Purity: Greater than 90.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Synonyms: Vascular Endothelial Growth Factor-C; Recombinant Rat Vascular Endothelial Growth Factor-C (152); VEGF C Rat (152 a.a.); Vascular Endothelial Growth Factor-C (152 a.a) Rat Recombinant; VEGF-C152; Vascular endothelial growth factor C 152; VRP; Flt4 ligand; Flt4-L; rVEGFC (152a.a.); rVEGFC active protein

• Host: Sf9 Insect Cells
• Purity/Purification: Greater than 90.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Form/Format: The protein was lyophilized from a concentrated (1mg/ml) solution with BSA.; Sterile Filtered White lyophilized (freeze-dried) powder.
• Sequence Length: 415

• Solubility: It is recommended to reconstitute the lyophilized Vascular Endothelial Growth Factor C 152 in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
• Biological Activity: Measured by its ability to stimulate phosphorylation of the VEGFR-3/FLT-4 receptor in porcine aortic endothelial cells (PAE/FLT -4 cells). The ED50 for this effect is typically 150-300 ng/ml, corresponding to a specific activity of 3.3-6.6KUnits/mg.
• Preparation and Storage: Lyophilized Vascular Endothelial Growth Factor-C152 although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution VEGF-C 152 should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.Please prevent freeze-thaw cycles.

Related Product Information for rVEGFC active protein

Description: Vascular Endothelial Growth Factor -C 152 Rat Recombinant contains 152 amino acids residues and was fused to a His-tag (6x His) at the C-terminal end. As a result of glycosylation VEGF-C migrates as an 18-24 kDa protein in SDS-PAGE under reducing conditions.

Introduction: VEGF-C152S is a point mutant generated by the replacement of the second conserved Cys residue of the recombinant processed VEGF-C by a Ser residue. VEGF-C 152S is analog to the human VEGF-C 156S mutant and only active toward VEGFR-3/FLT-4 but, unlike wild type VEGF-C, is unable to bind to and to activate signaling through VEGFR-2/KDR. VEGF-C152S was inactive in the vascular permeability assay and did not increase migration of the capillary endothelial cells, indicating that these VEGF-like effects of VEGF-C require VEGFR-2 binding. VEGF-C, also known as Vascular Endothelial Growth Factor Related Protein (VRP), is a recently discovered VEGF growth factor family member that is most closely related to VEGF-D. The rat VEGF-C cDNA encodes a pre-pro-protein of 416 amino acids residues. It is almost identical to the mouse VEGF-C protein. Similar to VEGF-D, VEGF-C has a VEGF homology domain spanning the middle third of the precursor molecule and long N- and C-terminal extensions. In adults, VEGF-C is highly expressed in heart, placenta, ovary and small intestine. Recombinant rat VEGF-C, lacking the N- and C-terminal extensions and containing only the middle VEGF homology domain, forms primarily non-covalently linked dimers. This protein is a ligand for both VEGFR-2/KDR and VEGFR-3/FLT -4. Since VEGFR-3 is strongly expressed in lymphatic endothelial cells, it has been postulated that VEGF-C is involved in the regulation of the growth and/or differentiation of lymphatic endothelium. Although recombinant rat VEGF-C is also a mitogen for vascular endothelial cells, it is much less potent than VEGF-A.

Product Categories/Family for rVEGFC active protein

Cytokines and Growth Factors; Growth Factors; VEGF

NCBI and Uniprot Product Information

• NCBI GI #: 16758992
• NCBI GeneID: 114111
• NCBI Accession #: NP_446105.1
• NCBI GenBank Nucleotide #: NM_053653.1
• UniProt Accession #: O35757; Q91ZE3
• Molecular Weight: 46,397 Da
• NCBI Official Full Name: vascular endothelial growth factor C
• NCBI Official Synonym Full Names: vascular endothelial growth factor C
• NCBI Official Symbol: Vegfc
• NCBI Protein Information: vascular endothelial growth factor C; VEGF-C; VRP; flt4 ligand; flt4-L; vascular endothelial growth factor-related protein
• UniProt Protein Name: Vascular endothelial growth factor C
• UniProt Gene Name: Vegfc
• UniProt Synonym Gene Names: VEGF-C; Flt4-L; VRP
• UniProt Entry Name: VEGFC_RAT

NCBI Description

platelet-derived growth factor/vascular derived growth factor (PDGF/VEGF); active in angiogenesis and endothelial cell growth [RGD, Feb 2006]

Uniprot Description

VEGFC: Growth factor active in angiogenesis, and endothelial cell growth, stimulating their proliferation and migration and also has effects on the permeability of blood vessels. May function in angiogenesis of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in adults. Binds and activates VEGFR-2 (KDR/FLK1) and VEGFR-3 (FLT4) receptors. Homodimer; non-covalent and antiparallel. Spleen, lymph node, thymus, appendix, bone marrow, heart, placenta, ovary, skeletal muscle, prostate, testis, colon and small intestine and fetal liver, lung and kidney, but not in peripheral blood lymphocyte. Belongs to the PDGF/VEGF growth factor family.

Protein type: Secreted; Secreted, signal peptide; Motility/polarity/chemotaxis; Cytokine; Cell cycle regulation

Cellular Component: extracellular space; membrane

Molecular Function: growth factor activity; vascular endothelial growth factor receptor 3 binding; chemoattractant activity

Biological Process: response to drug; positive regulation of neuroblast proliferation; positive regulation of blood vessel endothelial cell migration; positive regulation of protein amino acid autophosphorylation; regulation of angiogenesis; induction of positive chemotaxis; negative regulation of cell proliferation; organ morphogenesis; positive regulation of angiogenesis; positive regulation of peptidyl-tyrosine phosphorylation; morphogenesis of embryonic epithelium; positive chemotaxis; positive regulation of protein secretion; positive regulation of cell division; regulation of vascular endothelial growth factor receptor signaling pathway; positive regulation of cell proliferation; angiogenesis; negative regulation of blood pressure; positive regulation of cell-matrix adhesion; vascular endothelial growth factor receptor signaling pathway; positive regulation of epithelial cell proliferation