Vascular Endothelial Growth Factor-E Active Protein | VEGF active protein
Recombinant Vascular Endothelial Growth Factor-E (Orf Virus)
Purity
Greater than 90.0% as determined by SDS-PAGE.
Synonyms
Vascular Endothelial Growth Factor-E; Recombinant Vascular Endothelial Growth Factor-E (Orf Virus); VEGF E (Orf Virus); Vascular Endothelial Growth Factor-E Recombinant (Orf Virus); VEGF active protein
Host
E. coli
Purity/Purification
Greater than 90.0% as determined by SDS-PAGE.
Form/Format
The protein was lyophilized from a concentrated (1mg/ml) solution containing PBS.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Solubility
The lyophilized oVEGF-E Orf Virus should be reconstituted in water or medium to a concentration not lower than 50ug/ml. For long term storage we would recommend to add at least 0.1% human or bovine serum albumin.
Biological Activity
The biological activity was determined (1) by the ability to induce VEGFR-2/KDR receptor phosphorylation in PAE/KDR cells and (2) in a cell proliferation assay using primary HUVECs. The ED50 for this effect is typically 1-5ng/ml.
Preparation and Storage
Lyophilized Vascular Endothelial Growth Factor-E Orf Virus although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution VEGF E -OV should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.
Related Product Information for VEGF active protein
A DNA sequence encoding the mature variant of ovVEGF-E isolate D1701 (Dehio et al., 1999; GenBank accession No. AF106020) was expressed in E. coli as a 132 amino acid residue fusion protein with an N-terminal His-tag sequence and a thrombin cleavage site. Recombinant VEGF-E homodimer was dimerized in vitro and has a predicted mass of approximately 35 kDa.
Product Categories/Family for VEGF active protein
NCBI and Uniprot Product Information
Uniprot Description
Induces endothelial proliferation.
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Product Notes
The VEGF a2r (Catalog #AAA142140) is an Active Protein produced from E. coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Vascular Endothelial Growth Factor-E, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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