Granzyme-B Active Protein | mGZMB active protein

Recombinant Mouse Granzyme-B

Cat. Num. AAA141586

• Gene Names: Gzmb; GZB; CCP1; Ctla1; Ctla-1; AI553453; CCP-1/C11
• Purity: Greater than 90.0% as determined by SDS-PAGE.
• Synonyms: Granzyme-B; Recombinant Mouse Granzyme-B; mGZMB; GZMB Mouse; Granzyme-B Mouse Recombinant; Granzyme B; C11; CTLA-1; Cathepsin G-like 1; CTSGL1; Cytotoxic T-lymphocyte proteinase 2; Lymphocyte protease; Fragmentin-2; Granzyme-2; Human lymphocyte protein; HLP; SECT; T-cell serine protease 1-3E; CGL1; CSPB; CTLA1; GRB; GZMB; CCPI; CGL-1; CSP-B; mGZMB active protein

• Host: Sf9, Baculovirus cells
• Purity/Purification: Greater than 90.0% as determined by SDS-PAGE.
• Form/Format: Sterile filtered colorless solution.; The GZMB solution (0.25mg/ml) contains 10% Glycerol and Phosphate-Buffered Saline (pH7.4).
• Sequence: GEIIGGHEVK PHSRPYMALL SIKDQQPEAI CGGFLIREDF VLTAAHCEGS IINVTLGAHNIKEQEKTQQV IPMVKCIPHP DYNPKTFSND IMLLKLKSKA KRTRAVRPLN LPRRNVNVKPGDVCYVAGWG RMAPMGKYSN TLQEVELTVQ KDRECESYFK NRYNKTNQIC AGDPKTKRASFRGDSGGPLV CKKVAAGIVS YGYKDGSPPR AFTKVSSFLS WIKKTMKSSH HHHHH

• Biological Activity: Defined as the amount of enzyme that cleave 1pmole of Boc-Ala-Ala-Asp-SBzl at 37 degree C. Specific activity is >9,000pmol/min/ug.
• Preparation and Storage: Store at 4 degree C if entire vial will be used within 2-4 weeks.; Store, frozen at -20 degree C for longer periods of time.; For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).; Avoid multiple freeze-thaw cycles.

Related Product Information for mGZMB active protein

GZMB or Granzyme-B is a protein that mainly exist in granules of NK cells and T cells (cytotoxic), it is a serine protease. The protein is secreted by of NK cells and T cells together with the protein that creates pores (perforin) in order to lead to apoptosis in the target cell.
GZMB Mouse produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 235 amino acids (19-247 aa) and having a molecular mass of 26.3kDa.
GZMB is fused to a 6 amino acid His-Tag at C-terminus and purified by proprietary chromatographic techniques.

Product Categories/Family for mGZMB active protein

ENZYMES

NCBI and Uniprot Product Information

• NCBI GI #: 7305123
• NCBI GeneID: 14939
• NCBI Accession #: NP_038570.1
• NCBI GenBank Nucleotide #: NM_013542.3
• UniProt Accession #: P04187
• Molecular Weight: 27,470 Da
• NCBI Official Full Name: granzyme B(G,H) preproprotein
• NCBI Official Synonym Full Names: granzyme B
• NCBI Official Symbol: Gzmb
• NCBI Official Synonym Symbols: GZB; CCP1; Ctla1; Ctla-1; AI553453; CCP-1/C11
• NCBI Protein Information: granzyme B(G,H)
• UniProt Protein Name: Granzyme B(G,H)
• UniProt Gene Name: Gzmb
• UniProt Synonym Gene Names: Ctla-1; Ctla1; CCP1

NCBI Description

This gene encodes a member of the granzyme subfamily of proteins, part of the peptidase S1 family of serine proteases. The encoded preproprotein is secreted by natural killer (NK) cells and cytotoxic T lymphocytes (CTLs) and proteolytically processed to generate the active protease, which induces target cell apoptosis. This protein also processes cytokines and degrades extracellular matrix proteins, and these roles are implicated in chronic inflammation and wound healing. Mice lacking a functional copy of this gene exhibit impaired immune cell-mediated cytolysis. [provided by RefSeq, Sep 2016]

Uniprot Description

GZMB: This enzyme is necessary for target cell lysis in cell- mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis. By staphylococcal enterotoxin A (SEA) in peripheral blood leukocytes. Inactivated by the serine protease inhibitor diisopropylfluorophosphate. Belongs to the peptidase S1 family. Granzyme subfamily.

Protein type: Apoptosis; EC 3.4.21.79; Protease

Chromosomal Location of Human Ortholog: 14 C3|14 28.19 cM

Cellular Component: cytoplasm

Molecular Function: protein binding; serine-type endopeptidase activity; serine-type peptidase activity

Biological Process: induction of apoptosis by granzyme; protein processing; T cell mediated cytotoxicity