Kallikrein-3 Recombinant Protein | KLK3 recombinant protein

Recombinant Human Kallikrein-3

Cat. Num. AAA141433

• Gene Names: KLK3; APS; PSA; hK3; KLK2A1
• Purity: >95.0% as determined by:; (a) Analysis by RP-HPLC.; (b) Analysis by SDS-PAGE
• Synonyms: Kallikrein-3; Recombinant Human Kallikrein-3; Prostate-specific antigen; PSA; Gamma-seminoprotein; Seminin; P-30 antigen; Semenogelase; KLK3; APS; hK3; KLK2A1; KLK3 Protein; qKallikrein-3 Recombinant Human; KLK3 recombinant protein

• Host: E Coli
• Purity/Purification: >95.0% as determined by:; (a) Analysis by RP-HPLC.; (b) Analysis by SDS-PAGE
• Form/Format: Sterile filtered white lyophilized (freeze-dried) powder.; Lyophilized from a 0.2um filtered concentrated solution in 20mM Tris-HCl, pH 8.0, 150mM NaCl and 3% trehalose.
• Sequence: Amino acid: IVGGWECEKH SQPWQVLVAS RGRAVCGGVL VHPQWVLTAA HCIRNKSVIL LGRHSLFHPE DTGQVFQVSH SFPHPLYDMS LLKNRFLRPG DDSSHDLMLL RLSEPAELTDA VKVMDLPTQE PALGTTCYAS GWGSIEPEEF LTPKKLQCVD LHVISNDVCA QVHPQKVTKF MLCAGRWTGG KSTCSGDSGG PLVCNGVLQG ITSWGSEPCA LPERPSLYTK VVHYRKWIKD TIVANP.
• Sequence Length: 238

• Solubility: It is recommended to reconstitute the lyophilized Kallikrein-3 in sterile 18Momega-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
• Preparation and Storage: Lyophilized KLK3 although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C.; Upon reconstitution Kallikrein-3 should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.; Please prevent freeze-thaw cycles.

Related Product Information for KLK3 recombinant protein

Introduction: Kallikrein-3 (KLK3) is a part of the kallikrein-related peptidase family. Kallikreins are a subgroup of serine proteases having various physiological functions. Numerous kallikreins take part in carcinogenesis and some may be prospective cancer and other disease biomarkers. Kallikrein-3 is 1 of the 15 kallikrein subfamily members located in a cluster on chromosome 19 and is a protease present in seminal plasma. KLK3 hydrolyzes semenogelin-1 consequently leading to the liquefaction of the seminal coagulum. KLK3 acts normally in the liquefaction of seminal coagulum, probably by hydrolysis of the high molecular mass seminal vesicle protein. Serum level of the KLK3 protein, called PSA in the clinical setting, is beneficial in the identification and monitoring of prostatic carcinoma.

Description: Kallikrein-3 Human Recombinant produced in E.Coli is a single, non- glycosylated polypeptide chain containing 237 amino acids and having a molecular mass of 26.1kDa.KLK3 is purified by proprietary chromatographic techniques.

Product Categories/Family for KLK3 recombinant protein

ENZYMES

NCBI and Uniprot Product Information

• NCBI GI #: 71834853
• NCBI GeneID: 354
• NCBI Accession #: NP_001025218.1
• NCBI GenBank Nucleotide #: NP_001025218.1
• UniProt Accession #: P07288
• NCBI Official Full Name: prostate-specific antigen isoform 3 preproprotein
• NCBI Official Synonym Full Names: kallikrein related peptidase 3
• NCBI Official Symbol: KLK3
• NCBI Official Synonym Symbols: APS; PSA; hK3; KLK2A1
• NCBI Protein Information: prostate-specific antigen
• UniProt Protein Name: Prostate-specific antigen
• Protein Family: Glandular kallikrein
• UniProt Gene Name: KLK3
• UniProt Synonym Gene Names: APS; PSA; Seminin
• UniProt Entry Name: KLK3_HUMAN

NCBI Description

Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. The gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. It encodes a single-chain glycoprotein, a protease which is synthesized in the epithelial cells of the prostate gland, and is present in seminal plasma. It is thought to function normally in the liquefaction of seminal coagulum, presumably by hydrolysis of the high molecular mass seminal vesicle protein. The serum level of this protein, called PSA in the clinical setting, is useful in the diagnosis and monitoring of prostatic carcinoma. Alternate splicing of this gene generates several transcript variants encoding different isoforms. [provided by RefSeq, Dec 2019]

Uniprot Description

PSA: Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum. Forms heterodimer with SERPINA5. Inhibited by SERPINA5. Activity is strongly inhibited by Zn2+, 100 times more abundant in semen than in serum. This inhibition is relieved by exposure to semenogelins, which are avid zinc binders. Belongs to the peptidase S1 family. Kallikrein subfamily.

Protein type: Secreted; Protease; EC 3.4.21.77; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19q13.41

Cellular Component: extracellular region; nucleus

Molecular Function: protein binding; serine-type peptidase activity; serine-type endopeptidase activity

Biological Process: negative regulation of angiogenesis; cellular protein metabolic process; proteolysis