Rabbit anti-Human MYO1E Polyclonal Antibody | anti-MYO1E antibody

MYO1E, NT (MYO1E, MYO1C, Unconventional myosin-Ie, Myosin-Ic, Unconventional myosin 1E) (PE)

Cat. Num. AAA6323439

• Gene Names: MYO1E; FSGS6; MYO1C; HuncM-IC
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MYO1E; Polyclonal Antibody; NT (MYO1E; MYO1C; Unconventional myosin-Ie; Myosin-Ic; Unconventional myosin 1E) (PE); Unconventional myosin 1E; anti-MYO1E antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-MYO1E antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: MYO1E antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 227-257 amino acids from the N-terminal region of human MYO1E.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(MYO1E Antibody (N-term) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the MYO1E antibody detected the MYO1E protein (arrow).)

Related Product Information for anti-MYO1E antibody

Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails are presumed to bind to membranous compartments, which would be moved relative to actin filaments (By similarity).

Product Categories/Family for anti-MYO1E antibody

Antibodies; Myosin

References

Feeser, E.A., et al. Biochemistry 49(43):9353-9360(2010). Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Krendel, M., et al. FEBS Lett. 581(4):644-650(2007). Wang, A.G., et al. Biochem. Biophys. Res. Commun. 345(3):1022-1032(2006). El Mezgueldi, M., et al. J. Biol. Chem. 277(24):21514-21521(2002).

NCBI and Uniprot Product Information

• NCBI GI #: 55956916
• NCBI GeneID: 4643
• NCBI Accession #: NP_004989.2
• NCBI GenBank Nucleotide #: NM_004998.3
• UniProt Accession #: Q12965; Q14778
• Molecular Weight: 127,062 Da
• NCBI Official Full Name: unconventional myosin-Ie
• NCBI Official Synonym Full Names: myosin IE
• NCBI Official Symbol: MYO1E
• NCBI Official Synonym Symbols: FSGS6; MYO1C; HuncM-IC
• NCBI Protein Information: unconventional myosin-Ie
• UniProt Protein Name: Unconventional myosin-Ie
• Protein Family: Unconventional myosin
• UniProt Gene Name: MYO1E
• UniProt Synonym Gene Names: MYO1C
• UniProt Entry Name: MYO1E_HUMAN

NCBI Description

This gene encodes a member of the nonmuscle class I myosins which are a subgroup of the unconventional myosin protein family. The unconventional myosin proteins function as actin-based molecular motors. Class I myosins are characterized by a head (motor) domain, a regulatory domain and a either a short or long tail domain. Among the class I myosins, this protein is distinguished by a long tail domain that is involved in crosslinking actin filaments. This protein localizes to the cytoplasm and may be involved in intracellular movement and membrane trafficking. Mutations in this gene are the cause of focal segmental glomerulosclerosis-6. This gene has been referred to as myosin IC in the literature but is distinct from the myosin IC gene located on chromosome 17. [provided by RefSeq, Jan 2012]

Uniprot Description

MYO1E: Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails bind to membranous compartments, which are then moved relative to actin filaments. Binds to membranes containing anionic phospholipids via its tail domain. Required for normal morphology of the glomerular basement membrane, normal development of foot processes by kidney podocytes and normal kidney function. In dendritic cells, may control the movement of class II-containing cytoplasmic vesicles along the actin cytoskeleton by connecting them with the actin network via ARL14EP and ARL14. Defects in MYO1E are the cause of focal segmental glomerulosclerosis type 6 (FSGS6). A renal pathology defined by the presence of segmental sclerosis in glomeruli and resulting in proteinuria, reduced glomerular filtration rate and progressive decline in renal function. Renal insufficiency often progresses to end-stage renal disease, a highly morbid state requiring either dialysis therapy or kidney transplantation. FSGS6 is a childhood-onset disorder resulting in nephrotic syndrome, which includes massive proteinuria, hypoalbuminemia, hyperlipidemia, and edema.

Protein type: Motor; Motility/polarity/chemotaxis; Actin-binding

Chromosomal Location of Human Ortholog: 15q21-q22

Cellular Component: actin cytoskeleton; adherens junction; brush border; clathrin-coated endocytic vesicle; cytoplasm; cytoskeleton; intercellular junction; myosin complex

Molecular Function: actin filament binding; ATP binding; ATPase activity, coupled; calmodulin binding; microfilament motor activity; motor activity; phosphoinositide binding; protein binding

Biological Process: actin filament-based movement; endocytosis; glomerular basement membrane development; glomerular filtration; in utero embryonic development; nitrogen compound metabolic process; platelet-derived growth factor receptor signaling pathway; post-embryonic hemopoiesis; vasculogenesis

Disease: Focal Segmental Glomerulosclerosis 6