Interleukin 12 Active Protein | IL12 active protein

Recombinant Human Interleukin 12, CHO

Cat. Num. AAA141431

• Gene Names: IL12A; P35; CLMF; NFSK; NKSF1; IL-12A
• Purity: >97.0% as determined by analysis by SDS-PAGE.
• Synonyms: Interleukin 12; Recombinant Human Interleukin 12; CHO; 23-219aa (IL12A) / 23-328aa (IL12B); Interleukin 12 (subunit beta/alpha); IL12 His; NKSF1; NKSF; CTL maturation factor (TCMF); Cytotoxic lymphocyte maturation factor (CLMF); TSF; Edodekin-alpha; IL-12; IL12 Human; Interleukin-12 Human Recombinant; IL12 active protein

• Host: Baculovirus
• Purity/Purification: >97.0% as determined by analysis by SDS-PAGE.
• Form/Format: Sterile filtered white lyophilized (freeze-dried) powder.; Lyophilized from a 0.2um filtered concentrated solution in PBS, pH 7.2.
• Sequence: Amino acid: IL-12 is a heterodimer of IL-12A and IL-12B linked through a disulfide-bond:; ARNLPVATP DPGMFPCLHH SQNLLRAVSN MLQKARQTLE FYPCTSEEID HEDITKDKTS TVEACLPLEL TKNESCLNSR ETSFITNGSC LASRKTSFMM ALCLSSIYED LKMYQVEFKT MNAKLLMDPK RQIFLDQNML AVIDELMQAL NFNSETVPQK SSLEEPDFYK TKIKLCILLH AFRIRAVTID RVMSYLNAS.; IL-12B:; IWELKKDV YVVELDWYPD APGEMVVLTC DTPEEDGITW TLDQSSEVLG SGKTLTIQVK EFGDAGQYTC HKGGEVLSHS LLLLHKKEDG IWSTDILKDQ KEPKNKTFLR CEAKNYSGRF TCWWLTTIST DLTFSVKSSR GSSDPQGVTC GAATLSAERV RGDNKEYEYS VECQEDSACP AAEESLPIEV MVDAVHKLKY ENYTSSFFIR DIIKPDPPKN LQLKPLKNSR QVEVSWEYPD TWSTPHSYFS LTFCVQVQGK SKREKKDRVF TDKTSATVIC RKNASISVRA QDRYYSSSWS EWASVPCS.
• Sequence Length: 253

• Solubility: It is recommended to reconstitute the lyophilized Interleukin 12 in sterile 18Momega-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
• Biological Activity: Determined by cell proliferation assay using PHA-stimulated human T lymphoblasts. The ED50 for this effect is 0.01-0.05ng/mL corresponding to a specific activity of 1.1 × 104 units/?g, which is calibrated against rHuIL-12 WHO Standard.
• Preparation and Storage: Lyophilized Interleukin 12 although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C.; Upon reconstitution PDGF BB should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.; Please prevent freeze-thaw cycles.

Related Product Information for IL12 active protein

Introduction: Interleukin 12 subunit beta/alpha or IL12b/IL12a is a growth factor cytokine which increases the lytic activity of NK/lymphokine-activated killer cells, activated T and NK cells and prompt IFN-gamma production (through resting PBMC). IL12b/IL12a is an essential part to the process of cellular-immunity and activates the differentiation of Th1 cells originates from the precursor T helper cells. The protein is linked to IL23A and creates the IL-23 interleukin, by that, the autoimmune inflammation is induced and autoimmune inflammatory diseases and tumorigenesis are being affected.

Description: Interleukin 12 Human Recombinant is a glycosylated heterodimer containing 503 amino acids (306 amino acid rHuIL-12 p40 and the 197 amino acid rHuIL-12 p35 subunits) and having a molecular mass of 60kDa. IL12 is purified by proprietary chromatographic techniques.

Product Categories/Family for IL12 active protein

CYTOKINES AND GROWTH FACTORS

NCBI and Uniprot Product Information

• NCBI GI #: 4323579
• NCBI GeneID: 3592
• NCBI Official Full Name: interleukin 12
• NCBI Official Synonym Full Names: interleukin 12A
• NCBI Official Symbol: IL12A
• NCBI Official Synonym Symbols: P35; CLMF; NFSK; NKSF1; IL-12A
• NCBI Protein Information: interleukin-12 subunit alpha

NCBI Description

This gene encodes a subunit of a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. This cytokine is required for the T-cell-independent induction of interferon (IFN)-gamma, and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity. [provided by RefSeq, Jul 2008]