Argonaute 2 Recombinant Protein | AGO2 recombinant protein

Recombinant Human Argonaute 2 (1-200 a.a.)

Cat. Num. AAA141364

• Gene Names: AGO2; PPD; Q10; CASC7; EIF2C2; LINC00980
• Purity: >90.0% as determined by SDS-PAGE
• Synonyms: Argonaute 2; Recombinant Human Argonaute 2 (1-200 a.a.); Argonaute 2 (1-200 a.a.) Human Recombinant; Protein argonaute-2; Argonaute2; hAgo2; Argonaute RISC catalytic component 2; Eukaryotic translation initiation factor 2C 2; eIF-2C 2; eIF2C 2; PAZ Piwi domain protein; PPD; AGO2; EIF2C2; Protein slicer; AGO2 (1-200) Human; AGO2 recombinant protein

• Host: E Coli
• Purity/Purification: >90.0% as determined by SDS-PAGE
• Form/Format: Filtered White Lyophilized (Freeze-Dried) Powder; Filtered (0.4 um) and lyophilized from 0.5mg/ml in 50mM acetate buffer, pH 4.
• Sequence: MKHHHHHHAS MYSGAGPALA PPAPPPPIQG YAFKPPPRPD FGTSGRTIKL QANFFEMDIP KIDIYHYELD IKPEKCPRRV NREIVEHMVQ HFKTQIFGDR KPVFDGRKNL YTAMPLPIGR DKVELEVTLP GEGKDRIFKV SIKWVSCVSL QALHDALSGR LPSVPFETIQ ALDVVMRHLP SMRYTPVGRS FFTASEGCSN PLGGGREVW
• Sequence Length: 859

• Solubility: It is recommended to add 0.1M acetate buffer, pH 4 to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. AGO2 (1-200) is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
• Preparation and Storage: Store lyophilized protein at -20 degree C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles.; Reconstituted protein can be stored at 4 degree C for a limited period of time; it does not show any change after two weeks at 4 degree C.

Related Product Information for AGO2 recombinant protein

Introduction: The Argonaute protein is part of the RISC or RNA-induced silencing complex, as so, the protein has a key part in the slicing processes of RNA. The RNA interference (RNAi) is being held by RISC. Small non-coding RNA fragments bond to the Argonaute proteins, through base pairing, eventually leads to the cleavage of messenger RNA or translation suppression.

Description: AGO2 (1-200) Human Recombinant is a single, non-glycosylated, polypeptide chain containing 210 amino acids (1-200 a.a) and having a molecular mass of 23.7kDa (calculated). AGO2 (1-200) is fused to a 10 a.a His tag at N-terminal.

Product Categories/Family for AGO2 recombinant protein

Recombinant & Natural Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 229463006
• NCBI GeneID: 27161
• UniProt Accession #: Q9UKV8
• NCBI Official Full Name: Protein argonaute-2
• NCBI Official Synonym Full Names: argonaute RISC catalytic component 2
• NCBI Official Symbol: AGO2
• NCBI Official Synonym Symbols: PPD; Q10; CASC7; EIF2C2; LINC00980
• NCBI Protein Information: protein argonaute-2
• UniProt Protein Name: Protein argonaute-2
• Protein Family: Protein argonaute
• UniProt Gene Name: AGO2
• UniProt Synonym Gene Names: hAgo2
• UniProt Entry Name: AGO2_HUMAN

NCBI Description

This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]

Uniprot Description

AGO2: the endonuclease in RNA-induced silencing complexes (RISC) that cleaves siRNA/mRNA heteroduplexes bound to RISC. Ago2, along with Dicer and TRBP, are major components of RISC. Interacts with Dicer1 through its Piwi domain. Required for proper fibroblast growth factor signaling during gastrulation. Essential for embryonic development as well as RNA-mediated gene silencing (RNAi).

Protein type: RNA processing; RNA-binding; EC 3.1.26.n2

Chromosomal Location of Human Ortholog: 8q24

Cellular Component: nucleoplasm; polysome; mRNA cap complex; membrane; cytoplasm; ribonucleoprotein complex; cytosol

Molecular Function: mRNA binding; endoribonuclease activity; protein binding; miRNA binding; siRNA binding; translation initiation factor activity; metal ion binding; RNA 7-methylguanosine cap binding

Biological Process: epidermal growth factor receptor signaling pathway; negative regulation of translational initiation; Notch signaling pathway; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; nerve growth factor receptor signaling pathway; transcription, DNA-dependent; RNA interference, siRNA loading onto RISC; translation; miRNA-mediated gene silencing, mRNA cleavage; miRNA-mediated gene silencing, miRNA loading onto RISC; miRNA-mediated gene silencing, negative regulation of translation; post-embryonic development; regulation of transcription, DNA-dependent; pre-microRNA processing; translational initiation; innate immune response; RNA-mediated gene silencing; gene expression; posttranscriptional gene silencing