Transforming Growth Factor-Alpha Active Protein | TGFA active protein

Recombinant Human Transforming Growth Factor-Alpha

Cat. Num. AAA140971

• Gene Names: TGFA; TFGA
• Purity: Greater than 97.0% as determined by SDS-PAGE.
• Synonyms: Transforming Growth Factor-Alpha; Recombinant Human Transforming Growth Factor-Alpha; TGFA Human; Transforming Growth Factor-Alpha Human Recombinant; Transforming Growth Factor Alpha; Protransforming Growth Factor Alpha; TGF-Alpha; TGFA; TGFA active protein

• Host: E Coli
• Purity/Purification: Greater than 97.0% as determined by SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 um filtered solution in Acetonitrile and TFA.; Sterile Filtered White lyophilized (freeze-dried) powder.
• Sequence: VVSHFNDCPD SHTQFCFHGT CRFLVQEDKP ACVCHSGYVG ARCEHADLLA.
• Sequence Length: 159

• Biological Activity: The ED50, as measured in a proliferation assay using mouse Balb/3T3 cells, is less than 0.4ng/ml.
• Solubility: It is recommended to reconstitute the lyophilized TGFA in sterile 18M-cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
• Preparation and Storage: Lyophilized TGFA although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution TGFA should be stored at 4 degree C between 2-7 days and for future use below -18 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Related Product Information for TGFA active protein

Introduction: Transforming Growth Factor-Alpha (TGF-alpha) belongs to the EGF family of cytokines. TGFA soluble form is discharged from the membrane by proteolytic cleavage. Membrane-bound proTGF-alpha is biologically active and has a role in cell-cell adhesion or in the stimulation of adjacent cells. TGFA expression is common in transformed cells. Additionally, TGFA is expressed in normal tissues during embryogenesis and in adult cells/tissues, including the pituitary, keratinocytes, and macrophages.

Description: TGFA Human Recombinant (40-89) produced in E Coli is a single, non-glycosylated, polypeptide chain containing 50 amino acids and having a molecular mass of 6kDa.The TGFA is purified by proprietary chromatographic techniques.

Product Categories/Family for TGFA active protein

Cytokines and Growth Factors

NCBI and Uniprot Product Information

• NCBI GI #: 153791414
• NCBI GeneID: 7039
• NCBI Accession #: NP_001093161.1
• NCBI GenBank Nucleotide #: NM_001099691.2
• UniProt Accession #: P01135; Q15577; Q53SK7; Q9BS56; Q9UEI3; Q9UKM1; Q9UKM2; Q9UKM3; A8K286
• Molecular Weight: 17,329 Da
• NCBI Official Full Name: protransforming growth factor alpha isoform 2 preproprotein
• NCBI Official Synonym Full Names: transforming growth factor alpha
• NCBI Official Symbol: TGFA
• NCBI Official Synonym Symbols: TFGA
• NCBI Protein Information: protransforming growth factor alpha
• UniProt Protein Name: Protransforming growth factor alpha
• Protein Family: Transforming growth factor
• UniProt Gene Name: TGFA
• UniProt Synonym Gene Names: TGF-alpha; ETGF
• UniProt Entry Name: TGFA_HUMAN

Uniprot Description

TGFalpha: TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar. Interacts with the PDZ domains of MAGI3, SDCBP and SNTA1. The interaction with SDCBP, is required for the targeting to the cell surface. In the endoplasmic reticulum, in its immature form (i.e. with a prosegment and lacking full N-glycosylation), interacts with CNIH. In the Golgi apparatus, may form a complex with CNIH and GORASP2. Interacts (via cytoplasmic C-terminal domain) with NKD2. Isoform 1, isoform 3 and isoform 4 are expressed in keratinocytes and tumor-derived cell lines. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation; Ligand, receptor tyrosine kinase; Membrane protein, integral; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 2p13

Cellular Component: basolateral plasma membrane; cell surface; cytoplasmic vesicle; endoplasmic reticulum membrane; ER-Golgi intermediate compartment membrane; extracellular space; integral to plasma membrane; perinuclear region of cytoplasm

Molecular Function: epidermal growth factor receptor binding; growth factor activity; protein binding

Biological Process: activation of MAPK activity; cell proliferation; COPII coating of Golgi vesicle; ER to Golgi vesicle-mediated transport; positive regulation of epidermal growth factor receptor activity; positive regulation of epithelial cell proliferation; positive regulation of mitosis