Exosome Component 7 Recombinant Protein | EXOSC7 recombinant protein

Recombinant Human Exosome Component 7

Cat. Num. AAA140929

• Gene Names: EXOSC7; p8; EAP1; RRP42; Rrp42p; hRrp42p
• Purity: Greater than 85.0% as determined by SDS-PAGE.
• Synonyms: Exosome Component 7; Recombinant Human Exosome Component 7; EXOSC7 Human; Exosome Component 7 Human Recombinant; RRP42; Ribosomal RNA-Processing Protein 42; P8; Exosome Complex Exonuclease RRP42; Exosome Complex Component RRP42; KIAA0116; HRrp42p; EAP1; EXOSC7 recombinant protein

• Host: E Coli
• Purity/Purification: Greater than 85.0% as determined by SDS-PAGE.
• Form/Format: EXOSC7 protein solution (0.5mg/ml) containing 20mM Phosphate buffer saline (pH 8.0), 0.15M NaCl, 1mM DTT and 20% glycerol.; Sterile Filtered colorless solution.
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSMASVTLS EAEKVYIVHG VQEDLRVDGR GCEDYRCVEV ETDVVSNTSG SARVKLGHTD ILVGVKAEMG TPKLEKPNEG YLEFFVDCSA SATPEFEGRG GDDLGTEIAN TLYRIFNNKS SVDLKTLCIS PREHCWVLYV DVLLLECGGN LFDAISIAVK AALFNTRIPR VRVLEDEEGS KDIELSDDPY DCIRLSVENV PCIVTLCKIG YRHVVDATLQ EEACSLASLL VSVTSKGVVT CMRKVGKGSL DPESIFEMME TGKRVGKVLH ASLQSVVHKE ESLGPKRQKV GFLG
• Sequence Length: 291

• Preparation and Storage: Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

Related Product Information for EXOSC7 recombinant protein

Introduction: EXOSC7, a component of the RNA exosome complex, is a member of the RNase PH family. EXOSC7 takes part is several pathways such as transfer to a nascent protein and Unfolded Protein Response and Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO). A significant paralog of this gene is EXOSC9.

Description: EXOSC7 Human Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 314 amino acids (1-291 a.a.) and having a molecular mass of 34.2kDa.EXOSC7 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Categories/Family for EXOSC7 recombinant protein

Recombinant & Natural Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 189083688
• NCBI GeneID: 23016
• NCBI Accession #: NP_055819.2
• NCBI GenBank Nucleotide #: NM_015004.3
• UniProt Accession #: Q15024; Q96E72
• Molecular Weight: 31,821 Da
• NCBI Official Full Name: exosome complex component RRP42
• NCBI Official Synonym Full Names: exosome component 7
• NCBI Official Symbol: EXOSC7
• NCBI Official Synonym Symbols: p8; EAP1; RRP42; Rrp42p; hRrp42p
• NCBI Protein Information: exosome complex component RRP42
• UniProt Protein Name: Exosome complex component RRP42
• Protein Family: Exosome complex exonuclease
• UniProt Gene Name: EXOSC7
• UniProt Synonym Gene Names: KIAA0116; RRP42
• UniProt Entry Name: EXOS7_HUMAN

Uniprot Description

EXOSC7: Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes. Belongs to the RNase PH family.

Protein type: EC 3.1.13.-; Nucleolus; RNA processing; Ribonuclease

Chromosomal Location of Human Ortholog: 3p21.31

Cellular Component: cytosol; exosome (RNase complex); nuclear exosome (RNase complex); nucleoplasm; nucleus

Molecular Function: 3'-5'-exoribonuclease activity; AU-rich element binding; exoribonuclease activity; protein binding; RNA binding

Biological Process: exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); regulation of mRNA stability; rRNA processing