Aldehyde Dehydrogenase 2 Recombinant Protein | ALDH2 recombinant protein

Recombinant Mouse Aldehyde Dehydrogenase 2

Cat. Num. AAA140843

• Gene Names: Aldh2; Ahd5; ALDHI; Ahd-5; AHD-M1; ALDH-E2
• Purity: Greater than 85% as determined by SDS-PAGE.
• Synonyms: Aldehyde Dehydrogenase 2; Recombinant Mouse Aldehyde Dehydrogenase 2; ALDH2 Mouse; Aldehyde Dehydrogenase 2 Mouse Recombinant; Aldehyde dehydrogenase; mitochondrial; AHD-M1; ALDH class 2; ALDH-E2; ALDHI; ALDH2 recombinant protein

• Host: E Coli
• Purity/Purification: Greater than 85% as determined by SDS-PAGE.
• Form/Format: ALDH2 protein e solution (0.5mg/ml) containing Phosphate buffered salin(pH7.4), 20% glycerol and 1mM DTT.; Sterile Filtered clear solution.
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSSAAATSA VPAPNHQPEV FCNQIFINNE WHDAVSRKTF PTVNPSTGEVICQVAEGNKE DVDKAVKAAR AAFQLGSPWR RMDASDRGRL LYRLADLIER DRTYLAALET LDNGKPYVISYLVDLDMVLK CLRYYAGWAD KYHGKTIPID GDFFSYTRHE PVGVCGQIIP WNFPLLMQAW KLGPALATGNVVVMKVAEQT PLTALYVANL IKEAGFPPGV VNIVPGFGPT AGAAIASHEG VDKVAFTGST EVGHLIQVAAGSSNLKRVTL ELGGKSPNII MSDADMDWAV EQAHFALFFN QGQCCCAGSR TFVQENVYDE FVERSVARAKSRVVGNPFDS RTEQGPQVDE TQFKKILGYI KSGQQEGAKL LCGGGAAADR GYFIQPTVFG DVKDGMTIAKEEIFGPVMQI LKFKTIEEVV GRANDSKYGL AAAVFTKDLD KANYLSQALQ AGTVWINCYD VFGAQSPFGGYKMSGSGREL GEYGLQAYTE VKTVTVKVPQ KNS
• Sequence Length: 519

• Preparation and Storage: Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

Related Product Information for ALDH2 recombinant protein

Introduction: ALDH2 is part of the aldehyde dehydrogenase family of proteins which catalyze the chemical transformation from acetaldehyde to acetic acid. ALDH2 is the second enzyme of the major oxidative pathway of alcohol metabolism. ALDH2 has 2 major liver isoforms: cytosolic and mitochondrial, which differ by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Nearly all Caucasians have 2 major isozymes, whereas roughly 50% of Orientals have only the cytosolic isozyme, omitting the mitochondrial isozyme. The extremely higher rate of acute alcohol intoxication with Orientals compared to Caucasians is due to the fact of the absence of mitochondrial isozyme. ALDH2 has a low Km for acetaldehydes, and is localized in mitochondrial matrix.

Description: ALDH2 Mouse Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 523 amino acids (20-519 a.a) and having a molecular mass of 56.8kDa. ALDH2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Categories/Family for ALDH2 recombinant protein

Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 6753036
• NCBI GeneID: 11669
• NCBI Accession #: NP_033786.1
• NCBI GenBank Nucleotide #: NM_009656.4
• UniProt Accession #: P47738
• Molecular Weight: 56,538 Da
• NCBI Official Full Name: aldehyde dehydrogenase, mitochondrial isoform 1
• NCBI Official Synonym Full Names: aldehyde dehydrogenase 2, mitochondrial
• NCBI Official Symbol: Aldh2
• NCBI Official Synonym Symbols: Ahd5; ALDHI; Ahd-5; AHD-M1; ALDH-E2
• NCBI Protein Information: aldehyde dehydrogenase, mitochondrial
• UniProt Protein Name: Aldehyde dehydrogenase, mitochondrial
• Protein Family: Aldehyde dehydrogenase
• UniProt Gene Name: Aldh2
• UniProt Synonym Gene Names: Ahd-1; Ahd1
• UniProt Entry Name: ALDH2_MOUSE

Uniprot Description

ALDH2: This protein belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of aldehyde dehydrogenase, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have the cytosolic isozyme but not the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of a catalytically active form of the mitochondrial isozyme. The increased exposure to acetaldehyde in individuals with the catalytically inactive form may also confer greater susceptibility to many types of cancer. This gene encodes a mitochondrial isoform, which has a low Km for acetaldehydes, and is localized in mitochondrial matrix. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Mar 2011]

Protein type: Amino Acid Metabolism - arginine and proline; Amino Acid Metabolism - histidine; Amino Acid Metabolism - lysine degradation; Amino Acid Metabolism - tryptophan; Amino Acid Metabolism - valine, leucine and isoleucine degradation; Carbohydrate Metabolism - ascorbate and aldarate; Carbohydrate Metabolism - butanoate; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - propanoate; Carbohydrate Metabolism - pyruvate; EC 1.2.1.3; Lipid Metabolism - fatty acid; Lipid Metabolism - glycerolipid; Mitochondrial; Other Amino Acids Metabolism - beta-alanine; Oxidoreductase; Secondary Metabolites Metabolism - limonene and pinene degradation

Cellular Component: mitochondrion

Molecular Function: aldehyde dehydrogenase (NAD) activity; identical protein binding

Biological Process: negative regulation of apoptosis