RNA Guanylyltransferase And 5'-Phosphatase Recombinant Protein | RNGTT recombinant protein
Recombinant Human RNA Guanylyltransferase And 5'-Phosphatase
Gene Names
RNGTT; HCE; HCE1; hCAP; CAP1A
Purity
Greater than 85.0% as determined by SDS-PAGE.
Synonyms
RNA Guanylyltransferase And 5'-Phosphatase; Recombinant Human RNA Guanylyltransferase And 5'-Phosphatase; RNGTT Human; RNA Guanylyltransferase And 5'-Phosphatase Human Recombinant; CAP1A; RNA Guanylyltransferase And 5-Phosphatase; HCAP1; HCE1; HCE; MRNA-Capping Enzyme; HCAP 3; mRNA-capping enzyme; RNGTT recombinant protein
Host
E Coli
Purity/Purification
Greater than 85.0% as determined by SDS-PAGE.
Form/Format
RNGTT protein solution (0.25mg/ml) containing 20mM Tris-HCl buffer (pH 7.5), 0.2M NaCl, 40% glycerol, 2mM DTT and 0.1mM PMSF.
Sterile filtered colorless solution.
Sterile filtered colorless solution.
Sequence
MGSSHHHHHH SSGLVPRGSH MGSMAHNKIP PRWLNCPRRG QPVAGRFLPL KTMLGPRYDS QVAEENRFHPSMLSNYLKSL KVKMGLLVDL TNTSRFYDRN DIEKEGIKYI KLQCKGHGEC PTTENTETFI RLCERFNERNPPELIGVHCT HGFNRTGFLI CAFLVEKMDW SIEAAVATFAQARPPGIYKG DYLKELFRRY GDIEEAPPPP LLPDWCFEDD EDEDEDEDGKKESEPGSSAS FGKRRKERLK LGAIFLEGVT VKGVTQVTTQ PKLGEVQQKC HQFCGWEGSG FPGAQPVSMDKQNIKLLDLK PYKVSWKADG TRYMMLIDGT NEVFMIDRDN SVFHVSNLEF PFRKDLRMHLSNTLLDGEMI IDRVNGQAVP RYLIYDIIKF NSQPVGDCDF NVRLQCIEREIISPRHEKMK TGLIDKTQEP FSVRNKPFFD ICTSRKLLEG NFAKEVSHEM DGLIFQPTGK YKPGRCDDILKWKPPSLNSV DFRLKITRMG GEGLLPQNVG LLYVGGYERP FAQIKVTKEL KQYDNKIIECKFENNSWVFM RQRTDKSFPN AYNTAMAVCN SISNPVTKEM LFEFIDRCTAASQGQKRKHH LDPDTELMPP PPPKRPRPLT
Sequence Length
574
Preparation and Storage
Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Related Product Information for RNGTT recombinant protein
Introduction: RNA Guanylyltransferase And 5'-Phosphatase also known as RNGTT is a bifunctional mRNA-capping enzyme which exhibits RNA 5'-triphosphatase activity in the N-terminal section and mRNA guanylyltransferase activity in the C-terminal section. In addition, RNGTT catalyzes the first two steps of cap formation, through removing the gamma-phosphate from the 5'-triphosphate end of nascent mRNA to yield a diphosphate end, and also by transferring the gmp moiety of GTP to the 5'-diphosphate terminus.
Description: RNGTT Human Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 620 amino acids (1-597a.a) and having a molecular mass of 70.9kDa. RNGTT is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Description: RNGTT Human Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 620 amino acids (1-597a.a) and having a molecular mass of 70.9kDa. RNGTT is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Categories/Family for RNGTT recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
61,705 Da
NCBI Official Full Name
mRNA-capping enzyme isoform b
NCBI Official Synonym Full Names
RNA guanylyltransferase and 5'-phosphatase
NCBI Official Symbol
RNGTT
NCBI Official Synonym Symbols
HCE; HCE1; hCAP; CAP1A
NCBI Protein Information
mRNA-capping enzyme
UniProt Protein Name
mRNA-capping enzyme
UniProt Gene Name
RNGTT
UniProt Synonym Gene Names
CAP1A; TPase; GTase
UniProt Entry Name
MCE1_HUMAN
Uniprot Description
RNGTT: a bifunctional mRNA capping enzyme with RNA 5'-triphosphatase activity in the N-terminal part and mRNA guanyltransferase activity in the C-terminal part. Catalyzes the first two steps of cap formation: by removing the gamma-phosphate from the 5'-triphosphate end of nascent mRNA to yield a diphosphate end, and by transferring the gmp moiety of GTP to the 5'-diphosphate terminus. Four alternatively spliced human isoforms have been described. Isoform 1 and isoform 4 are expressed in cerebrum, cerebellum, thyroid, lung, heart, liver, kidney, spleen, large intestine, testis, skin and muscle. Isoforms 2, 3 and 4 lack mRNA 5'-guanylyltransferase activity due to disruptions of the GTPase domain.
Protein type: EC 2.7.7.50; EC 3.1.3.33; Motility/polarity/chemotaxis; Phosphatase (non-protein); Transferase
Chromosomal Location of Human Ortholog: 6q16
Cellular Component: nucleoplasm; nucleus
Molecular Function: mRNA guanylyltransferase activity; polynucleotide 5'-phosphatase activity; RNA guanylyltransferase activity; triphosphatase activity
Biological Process: mRNA capping; RNA processing; transcription from RNA polymerase II promoter
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Product Notes
The RNGTT rngtt (Catalog #AAA140783) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: MGSSHHH HHH SSGLVPRGSH MGSMAH NKIP PRWLNCPRRG QPVAGRFLPL KTMLGPRYDS QVAEENRFHP SMLSNYLKSL KVKMGLLVDL TNTSRFYDRN DIEKEGIKYI KLQCKGHGEC PTTENTETFI RLCERFNERN PPELIGVHCT HGFNRTGFLI CAFLVEKMDW SIEAAVATFA QARPPGIYKG DYLKELFRRY GDIEEAPPPP LLPDWCFEDD EDEDEDEDGK KESEPGSSAS FGKRRKERLK LGAIFLEGVT VKGVTQVTTQ PKLGEVQQKC HQFCGWEGSG FPGAQPVSMD KQNIKLLDLK PYKVSWKADG TRYMMLIDGT NEVFMIDRDN SVFHVSNLEF PFRKDLRMHL SNTLLDGEMI IDRVNGQAVP RYLIYDIIKF NSQPVGDCDF NVRLQCIERE IISPRHEKMK TGLIDKTQEP FSVRNKPFFD ICTSRKLLEG NFAKEVSHEM DGLIFQPTGK YKPGRCDDIL KWKPPSLNSV DFRLKITRMG GEGLLPQNVG LLYVGGYERP FAQIKVTKEL KQYDNKIIEC KFENNSWVFM RQRTDKSFPN AYNTAMAVCN SISNPVTKEM LFEFIDRCTA ASQGQKRKHH LDPDTELMPP PPPKRPRPLT. It is sometimes possible for the material contained within the vial of "RNA Guanylyltransferase And 5'-Phosphatase, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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