Lipase Endothelial Recombinant Protein | LIPG recombinant protein

Recombinant Human Lipase Endothelial, HEK

Cat. Num. AAA140744

• Gene Names: LIPG; EL; EDL; PRO719
• Purity: Purity as determined by densitometric image analysis is greater than 85%.
• Synonyms: Lipase Endothelial; Recombinant Human Lipase Endothelial; HEK; LIPG Human; Lipase Endothelial Human Recombinant; LIPG; EDL; EL; Endothelial Cell-Derived Lipase; EC 3.1.1.3; PRO719; Endothelial Lipase; Lipoprotein Lipase H; EC 3.1.1; LIPG recombinant protein

• Host: HEK 293
• Purity/Purification: Purity as determined by densitometric image analysis is greater than 85%.
• Form/Format: LIPG was filtered (0.4 um) and lyophilized from 0.5mg/ml solution in phosphate buffered saline pH 7.5 (PBS), 1% (w/v) Sucrose and 4% (w/v) Mannitol.; Filtered White lyophilized (freeze-dried) powder.
• Sequence: ASSPVPFGPE GRLEDKLHKP KATQTEVKPS VRFNLRTSKD PEHEGCYLSV GHSQPLEDCS FNMTAKTFFI IHGWTMSGIF ENWLHKLVSA LHTREKDANV VVVDWLPLAH QLYTDAVNNT RVVGHSIARM LDWLQEKDDF SLGNVHLIGY SLGAHVAGYA GNFVKGTVGR ITGLDPAGPM FEGADIHKRL SPDDADFVDV LHTYTRSFGL SIGIQMPVGH IDIYPNGGDF QPGCGLNDVL GSIAYGTITE VVKCEHERAV HLFVDSLVNQ DKPSFAFQCT DSNRFKKGIC LSCRKNRCNS IGYNAKKMRN KRNSKMYLKT RAGMPFRVYH YQMKIHVFSY KNMGEIEPTF YVTLYGTNAD SQTLPLEIVE RIEQNATNTF LVYTEEDLGD LLKIQLTWEG ASQSWYNLWK EFRSYLSQPR NPGRELNIRR IRVKSGETQR KLTFCTEDPE NTSISPGREL WFRKCRDGWR MKNETSPTVE LP KLHHHHHH
• Sequence Length: 426

• Solubility: It is recommended to add 200 ul deionized water to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. LIPG is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
• Preparation and Storage: Store lyophilized protein at -20 degree C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4 degree C for a limited period of time; it does not show any change after two weeks at 4 degree C.

Related Product Information for LIPG recombinant protein

Introduction: Lipase Endothelial (LIPG) has extensive phospholipase activity and may be involved in lipoprotein metabolism and vascular biology. The LIPG protein is considered a member of the TG lipase family through its sequence and characteristic lid region which provides substrate specificity for enzymes of the TG lipase family. In addition, the LIPG has triglyceride lipase activities. LIPG hydrolyzes HDLs more efficiently than other lipoproteins. LIPG also binds heparin.

Description: LIPG Human Recombinant produced in HEK cells is a single, glycosylated, polypeptide chain (Ser21-Pro500) containing a total of 490 amino acids, having a calculated molecular mass of 55.8kDa. LIPG is fused to a 2 aa N-terminal linker, a 2 aa C-terminal linker and a 6 aa His tag at C-Terminus.

Product Categories/Family for LIPG recombinant protein

Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 808175995
• NCBI GeneID: 9388
• NCBI Accession #: NP_001294935.1
• NCBI GenBank Nucleotide #: NM_001308006.1
• UniProt Accession #: Q9Y5X9; Q6P9C8; Q6UW82; B0LPG6
• Molecular Weight: 39,362 Da
• NCBI Official Full Name: endothelial lipase isoform 2
• NCBI Official Synonym Full Names: lipase G, endothelial type
• NCBI Official Symbol: LIPG
• NCBI Official Synonym Symbols: EL; EDL; PRO719
• NCBI Protein Information: endothelial lipase
• UniProt Protein Name: Endothelial lipase
• Protein Family: Endothelial lipase
• UniProt Gene Name: LIPG
• UniProt Synonym Gene Names: EDL; EL
• UniProt Entry Name: LIPE_HUMAN

Uniprot Description

LIPG: Has phospholipase and triglyceride lipase activities. Hydrolyzes high density lipoproteins (HDL) more efficiently than other lipoproteins. Binds heparin. Belongs to the AB hydrolase superfamily. Lipase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.1.3; Lipid Metabolism - glycerolipid; Phospholipase; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 18q21.1

Cellular Component: cell surface; early endosome; extracellular region; extracellular space; Golgi apparatus

Molecular Function: lipoprotein lipase activity; phospholipase A1 activity; phospholipase activity

Biological Process: cholesterol homeostasis; lipid digestion; lipid metabolic process; phospholipid homeostasis; positive regulation of cholesterol transport; reverse cholesterol transport