Neuroepithelial cell-transforming gene 1 Recombinant Protein | NET1 recombinant protein

Recombinant Human Neuroepithelial cell-transforming gene 1 protein

Cat. Num. AAA1406386

• Gene Names: NET1; NET1A; ARHGEF8
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Neuroepithelial cell-transforming gene 1; Recombinant Human Neuroepithelial cell-transforming gene 1 protein; Proto-oncogene p65 Net1; Rho guanine nucleotide exchange factor 8; NET1 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1-542aa; Full Length of Isoform 2
• Sequence: MVAHDETGGLLPIKRTIRVLDVNNQSFREQEEPSNKRVRPLARVTSLANLISPVRNGAVRRFGQTIQSFTLRGDHRSPASAQKFSSRSTVPTPAKRRSSALWSEMLDITMKESLTTREIRRQEAIYEMSRGEQDLIEDLKLARKAYHDPMLKLSIMSEEELTHIFGDLDSYIPLHEDLLTRIGEATKPDGTVEQIGHILVSWLPRLNAYRGYCSNQLAAKALLDQKKQDPRVQDFLQRCLESPFSRKLDLWSFLDIPRSRLVKYPLLLKEILKHTPKEHPDVQLLEDAILIIQGVLSDINLKKGESECQYYIDKLEYLDEKQRDPRIEASKVLLCHGELRSKSGHKLYIFLFQDILVLTRPVTRNERHSYQVYRQPIPVQELVLEDLQDGDVRMGGSFRGAFSNSEKAKNIFRIRFHDPSPAQSHTLQANDVFHKQQWFNCIRAAIAPFQSAGSPPELQGLPELHEECEGNHPSARKLTAQRRASTVSSVTQVEVDENAYRCGSGMQMAEDSKSLKTHQTQPGIRRARDKALSGGKRKETLV
• Sequence Length: 596

• Preparation and Storage: Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.

SDS-PAGE

Related Product Information for NET1 recombinant protein

Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase. May be involved in activation of the SAPK/JNK pathway Stimulates genotoxic stress-induced RHOB activity in breast cancer cells leading to their cell death.

Product Categories/Family for NET1 recombinant protein

Signal Transduction

References

Isolation of a novel oncogene, NET1, from neuroepithelioma cells by expression cDNA cloning.Chan A.M.-L., Takai S., Yamada K., Miki T.Oncogene 12:1259-1266(1996) The activity of guanine exchange factor NET1 is essential for transforming growth factor-beta-mediated stress fiber formation.Shen X., Li J., Hu P.P.-C., Waddell D., Zhang J., Wang X.-F.J. Biol. Chem. 276:15362-15368(2001) A quantitative atlas of mitotic phosphorylation.Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.Sci. Signal. 2:RA46-RA46(2009) The nuclear guanine nucleotide exchange factors Ect2 and Net1 regulate RhoB-mediated cell death after DNA damage.Srougi M.C., Burridge K.PLoS ONE 6:E17108-E17108(2011) N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB.Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) Crystal structure of the RhoGEF domain of human neuroepithelial cell-transforming gene 1 protein.Structural genomics consortium (SGC) Submitted (FEB-2009) to the PDB data bankThe consensus coding sequences of human breast and colorectal cancers.Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V., Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E.Science 314:268-274(2006)

NCBI and Uniprot Product Information

• NCBI GI #: 114145479
• NCBI GeneID: 10276
• NCBI Accession #: NP_001040625.1
• NCBI GenBank Nucleotide #: NM_001047160.2
• UniProt Accession #: Q7Z628; Q12773; Q96D82; Q99903; Q9UEN6
• Molecular Weight: 77.9 kDa
• NCBI Official Full Name: neuroepithelial cell-transforming gene 1 protein isoform 1
• NCBI Official Synonym Full Names: neuroepithelial cell transforming 1
• NCBI Official Symbol: NET1
• NCBI Official Synonym Symbols: NET1A; ARHGEF8
• NCBI Protein Information: neuroepithelial cell-transforming gene 1 protein
• UniProt Protein Name: Neuroepithelial cell-transforming gene 1 protein
• Protein Family: Nucleolar protein
• UniProt Gene Name: NET1
• UniProt Synonym Gene Names: ARHGEF8
• UniProt Entry Name: ARHG8_HUMAN

NCBI Description

This gene is part of the family of Rho guanine nucleotide exchange factors. Members of this family activate Rho proteins by catalyzing the exchange of GDP for GTP. The protein encoded by this gene interacts with RhoA within the cell nucleus and may play a role in repairing DNA damage after ionizing radiation. Pseudogenes of this gene are located on the long arms of chromosomes 1, 7 and 18. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Jul 2012]

Uniprot Description

NET1: Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase. May be involved in activation of the SAPK/JNK pathway Stimulates genotoxic stress-induced RHOB activity in breast cancer cells leading to their cell death. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: GEFs; GEFs, Rac/Rho; Motility/polarity/chemotaxis; Oncoprotein

Chromosomal Location of Human Ortholog: 10p15

Cellular Component: cytosol

Molecular Function: guanyl-nucleotide exchange factor activity; Rho guanyl-nucleotide exchange factor activity

Biological Process: positive regulation of apoptosis; positive regulation of GTPase activity; regulation of small GTPase mediated signal transduction; signal transduction