Mouse V-maf Musculoaponeurotic Fibrosarcoma Oncogene K Monoclonal Antibody | anti-MAFK antibody

Mouse Anti Human V-maf Musculoaponeurotic Fibrosarcoma Oncogene K

Cat. Num. AAA140442

• Gene Names: MAFK; P18; NFE2U
• Applications: ELISA, Western Blot
• Purity: MAFK antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
• Synonyms: V-maf Musculoaponeurotic Fibrosarcoma Oncogene K; Monoclonal Antibody; Mouse Anti Human V-maf Musculoaponeurotic Fibrosarcoma Oncogene K; MAFK Antibody; Mouse Anti Human; NFE2U; P18; Erythroid transcription factor NF-E2 p18 subunit; MAFK; Transcription factor MafK; anti-MAFK antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a heavy chain and Kappa light chain
• Clone Number: PAT2F7A
• Purity/Purification: MAFK antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
• Form/Format: 1mg/ml containing PBS, pH-7.4, 10% Glycerol and 0.02% Sodium Azide.; Sterile Filtered colorless solution.
• Sequence Length: 156

• Applicable Applications for anti-MAFK antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:1000.
• Immunogen: Anti-human MAFK mAb, clone PAT2F7A, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human MAFK protein 1-156 amino acids purified from E Coli.
• Preparation and Storage: For periods up to 1 month store at 4 degree C, for longer periods of time, store at -20 degree C. Prevent freeze thaw cycles.; 12 months at -20 degree C. 1 month at 4 degree C.

Related Product Information for anti-MAFK antibody

Introduction: MAFK is a member of the bZIP family and Maf subfamily. Members from Maf family of basic region/leucine zipper (bZIP) transcription factors influence transcription in a positive or negative way, depending on their protein associate and the context of the target promoter. Small Mafs act as transcriptional repressors when they dimerize among themselves. Yet, they seem to function as transcriptional activators by dimerizing with other basic-zipper proteins and employing them to specific DNA-binding sites.

Product Categories/Family for anti-MAFK antibody

Monoclonal/Polyclonal Antibodies; Monoclonal Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 187950323
• NCBI GeneID: 7975
• Molecular Weight: 17,523 Da
• NCBI Official Full Name: V-maf musculoaponeurotic fibrosarcoma oncogene homolog K (avian)
• NCBI Official Synonym Full Names: v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog K
• NCBI Official Symbol: MAFK
• NCBI Official Synonym Symbols: P18; NFE2U
• NCBI Protein Information: transcription factor MafK; basic-leucine zipper transcription factor MafK; erythroid transcription factor NF-E2 p18 subunit; nuclear factor erythroid-2, ubiquitous (p18); v-maf avian musculoaponeurotic fibrosarcoma oncogene family, protein K; v-maf musculoaponeurotic fibrosarcoma oncogene homolog K
• UniProt Protein Name: Transcription factor MafK
• Protein Family: Transcription factor
• UniProt Gene Name: MAFK
• UniProt Entry Name: MAFK_HUMAN

NCBI Description

The developmentally regulated expression of the globin genes depends on upstream regulatory elements termed locus control regions (LCRs). LCRs are associated with powerful enhancer activity that is mediated by the transcription factor NFE2 (nuclear factor erythroid-2). NFE2 recognition sites are also present in the gene promoters of 2 heme biosynthetic enzymes, porphobilinogen deaminase (PBGD; MIM 609806) and ferrochelatase (FECH; MIM 612386). NFE2 DNA-binding activity consists of a heterodimer containing an 18-kD Maf protein (MafF, MafG (MIM 602020), or MafK) and p45 (MIM 601490). Both subunits are members of the activator protein-1 superfamily of basic leucine zipper (bZIP) proteins (see MIM 165160). Maf homodimers suppress transcription at NFE2 sites.[supplied by OMIM, Nov 2008]

Uniprot Description

MAFK: Since they lack a putative transactivation domain, the small Mafs behave as transcriptional repressors when they dimerize among themselves. However, they seem to serve as transcriptional activators by dimerizing with other (usually larger) basic-zipper proteins and recruiting them to specific DNA-binding sites. Small Maf proteins heterodimerize with Fos and may act as competitive repressors of the NF-E2 transcription factor. Belongs to the bZIP family. Maf subfamily.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 7p22.3

Cellular Component: nucleoplasm

Molecular Function: transcription factor activity

Biological Process: nervous system development; transcription, DNA-dependent; regulation of transcription, DNA-dependent; blood coagulation