Mouse Phenylethanolamine-N-Methyltransferase Monoclonal Antibody | anti-PNMT antibody
Mouse Anti Human Phenylethanolamine-N-Methyltransferase
Gene Names
PNMT; PENT; PNMTase
Applications
ELISA, Western Blot
Purity
PNMT antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
Synonyms
Phenylethanolamine-N-Methyltransferase; Monoclonal Antibody; Mouse Anti Human Phenylethanolamine-N-Methyltransferase; PNMT Antibody; Mouse Anti Human; PENT; PNMTase; Noradrenaline-N-methyltransferase; Phenylethanolamine N-methyltransferase; PNMT; MGC34570; anti-PNMT antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1 heavy chain and k light chain
Clone Number
PAT1C11
Purity/Purification
PNMT antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
Form/Format
1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide.
Sequence Length
282
Applicable Applications for anti-PNMT antibody
ELISA (EIA), Western Blot (WB)
Application Notes
The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500.
Immunogen
Anti-human PNMT, is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with recombinant human ADK amino acids 1-282 purified from E Coli.
Related Product Information for anti-PNMT antibody
Introduction: PNMT is an enzyme located in the adrenal medulla and it catalyzes the final step of the catecholamine biosynthesis pathway, which methylates norepinephrine to form epinephrine (adrenaline). PNMT has beta-carboline 2N-methyltransferase activity. PNMT takes part in regulating epinephrine production. Glucocorticoid receptors form multimers of PNMT independent of the DNA binding domain. PNMT expression is regulated late in mouse gestation by AP2-alpha and glucocorticoids.
Product Categories/Family for anti-PNMT antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
30,855 Da
NCBI Official Full Name
phenylethanolamine N-methyltransferase
NCBI Official Synonym Full Names
phenylethanolamine N-methyltransferase
NCBI Official Symbol
PNMT
NCBI Official Synonym Symbols
PENT; PNMTase
NCBI Protein Information
phenylethanolamine N-methyltransferase; noradrenaline N-methyltransferase; phenylethanolamine N-methylase
UniProt Protein Name
Phenylethanolamine N-methyltransferase
Protein Family
UniProt Gene Name
PNMT
UniProt Synonym Gene Names
PENT; PNMTase
UniProt Entry Name
PNMT_HUMAN
NCBI Description
The product of this gene catalyzes the last step of the catecholamine biosynthesis pathway, which methylates norepinephrine to form epinephrine (adrenaline). The enzyme also has beta-carboline 2N-methyltransferase activity. This gene is thought to play a key step in regulating epinephrine production. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Nov 2012]
Uniprot Description
PNMT: Converts noradrenaline to adrenaline. Belongs to the NNMT/PNMT/TEMT family.
Protein type: Amino Acid Metabolism - tyrosine; Methyltransferase; EC 2.1.1.28
Chromosomal Location of Human Ortholog: 17q12
Cellular Component: cytosol
Molecular Function: phenylethanolamine N-methyltransferase activity
Biological Process: methylation; catecholamine biosynthetic process; epinephrine biosynthetic process
Disease: Hypertension, Essential
Research Articles on PNMT
Similar Products
Product Notes
The PNMT pnmt (Catalog #AAA140387) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Phenylethanolamine-N-Methyltransferase can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500. Researchers should empirically determine the suitability of the PNMT pnmt for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Phenylethanolamine-N-Methyltransferase, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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