Mouse Programmed Cell Death 4 Monoclonal Antibody | anti-PDCD4 antibody

Mouse Anti Human Programmed Cell Death 4

Cat. Num. AAA140299

• Gene Names: PDCD4; H731
• Applications: ELISA, Western Blot
• Purity: PDCD4 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
• Synonyms: Programmed Cell Death 4; Monoclonal Antibody; Mouse Anti Human Programmed Cell Death 4; PDCD4 Antibody; Mouse Anti Human; Programmed cell death protein 4; Nuclear antigen H731-like; Neoplastic transformation inhibitor protein; Protein 197/15a; PDCD4; H731; MGC33046; MGC33047; anti-PDCD4 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1 heavy chain and kappa light chain
• Clone Number: Pk4C1AT
• Purity/Purification: PDCD4 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
• Form/Format: 1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide.
• Sequence Length: 469

• Applicable Applications for anti-PDCD4 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: PDCD4 antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1,000~ 3,000. Recommended starting dilution is 1:2,000.
• Immunogen: Anti-human PDCD4 mAb is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with recombinant human PDCD4 amino acids 1-469 purified from E Coli.
• Preparation and Storage: For periods up to 1 month store at 4 degree C, for longer periods of time, store at -20 degree C. Prevent freeze thaw cycles.; 12 months at -20 degree C. 1 month at 4 degree C.

Related Product Information for anti-PDCD4 antibody

Introduction: PDCD4 is restricted to the nucleus in proliferating cells. Expression of PDCD4 is modulated by cytokines in natural killer and T cells. PDCD4 takes part in apoptosis. PDCD4 is a translation inhibitor targeted for degradation during tumor promotion. PDCD4 promotes colonic neoplastic transformation and tumor invasion. PDCD4 is an important target for microrna R-21 in breast cancer cells. Shortage of PDCD4 expression is associated with colorectal cancer. PDCD4 is swiftly phosphorylated by S6K1 kinase protein & afterwords degraded by ubiquitin ligase SCF in response to mitogens. PDCD4 in response to mitogens allows competent protein synthesis & cell growth. PDCD4 is a proapoptotic protein involved in TGF-beta1-induced apoptosis in human HCC cells, and a tumor suppressor in hepatocarcinogenesis. PDCD4 restraines tumor progression in colon carcinoma cells by the novel mechanism of down-regulating MAP4K1 transcription, with consequent inhibition of c-Jun activation and AP-1-dependent transcri

Product Categories/Family for anti-PDCD4 antibody

Monoclonal/Polyclonal Antibodies; Monoclonal Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 21735596
• NCBI GeneID: 27250
• NCBI Accession #: NP_055271.2
• NCBI GenBank Nucleotide #: NM_014456.4
• Molecular Weight: 50,576 Da
• NCBI Official Full Name: programmed cell death protein 4 isoform 1
• NCBI Official Synonym Full Names: programmed cell death 4 (neoplastic transformation inhibitor)
• NCBI Official Symbol: PDCD4
• NCBI Official Synonym Symbols: H731
• NCBI Protein Information: programmed cell death protein 4; neoplastic transformation inhibitor protein; nuclear antigen H731; protein 197/15a
• UniProt Protein Name: Programmed cell death protein 4
• Protein Family: Programmed cell death protein
• UniProt Gene Name: PDCD4
• UniProt Synonym Gene Names: H731
• UniProt Entry Name: PDCD4_HUMAN

NCBI Description

This gene is a tumor suppressor and encodes a protein that binds to the eukaryotic translation initiation factor 4A1 and inhibits its function by preventing RNA binding. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2010]

Uniprot Description

PDCD4: protein localized to the nucleus in proliferating cells that seems to possess a tumor suppressor activity. Directly interacts with the RNA helicase eIF4A and inhibits protein synthesis by interfering with the assembly of the cap-dependent translation initiation complex. Suppresses carbonic anhydrase type II protein expression in carcinoid cell lines. Since tumor cells require a high bicarbonate flux for their growth, carbonic anhydrase suppression results in growth inhibition. Expression of this gene is modulated by cytokines in natural killer and T cells. The gene product is thought to play a role in apoptosis but the specific role has not yet been determined. Two differentially spliced isoforms have been identified.

Protein type: Apoptosis; Tumor suppressor

Chromosomal Location of Human Ortholog: 10q24

Cellular Component: nucleoplasm; cytoplasm; cytosol; nucleus

Molecular Function: protein binding; RNA binding

Biological Process: negative regulation of JNK activity; apoptosis; cell aging; negative regulation of transcription, DNA-dependent; negative regulation of cell cycle