Mouse Nuclear factor of activated T-cells cytoplasmic 1 Monoclonal Antibody | anti-NFATC1 antibody

Mouse Anti Human Nuclear factor of activated T-cells cytoplasmic 1

Cat. Num. AAA140291

• Gene Names: NFATC1; NFAT2; NFATc; NF-ATC
• Applications: ELISA, Western Blot
• Purity: NFATC1 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
• Synonyms: Nuclear factor of activated T-cells cytoplasmic 1; Monoclonal Antibody; Mouse Anti Human Nuclear factor of activated T-cells cytoplasmic 1; NFATC1 Antibody; Mouse Anti Human; NFAT transcription complex cytosolic component; NF-ATc1; NFATc1; NF-ATc; NFATc; NFAT2; MGC138448; anti-NFATC1 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a heavy chain and kappa light chain
• Clone Number: PAT1C3AT
• Purity/Purification: NFATC1 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
• Form/Format: 1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide.
• Sequence Length: 825

• Applicable Applications for anti-NFATC1 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: NFATC1 antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1000 ~ 2000. Recommended starting dilution is 1:1000.
• Immunogen: Anti-human NFATC1 mAb is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human NFATC1 amino acids 428-716 purified from E Coli.
• Preparation and Storage: For periods up to 1 month store at 4 degree C, for longer periods of time, store at -20 degree C. Prevent freeze thaw cycles.; 12 months at -20 degree C. 1 month at 4 degree C.

Related Product Information for anti-NFATC1 antibody

Introduction: NFATC1 is a component of the nuclear factor of activated T cells DNA-binding transcription complex. This complex consists of at least 2 components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation, and an inducible nuclear component. Proteins belonging to this family of transcription factors play an essential role in inducible gene transcription during immune response. NFATC1 is an inducible nuclear component. NFATC1 functions as a major molecular target for the immunosuppressive drugs such as cyclosporin A. Different isoforms of NFATC1 may regulate inducible expression of different cytokine genes.

Product Categories/Family for anti-NFATC1 antibody

Monoclonal/Polyclonal Antibodies; Monoclonal Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 19923350
• NCBI GeneID: 4772
• NCBI Accession #: NP_006153.2
• NCBI GenBank Nucleotide #: NM_006162.4
• Molecular Weight: 38,626 Da
• NCBI Official Full Name: nuclear factor of activated T-cells, cytoplasmic 1 isoform B
• NCBI Official Synonym Full Names: nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1
• NCBI Official Symbol: NFATC1
• NCBI Official Synonym Symbols: NFAT2; NFATc; NF-ATC
• NCBI Protein Information: nuclear factor of activated T-cells, cytoplasmic 1; NFAT transcription complex cytosolic component
• UniProt Protein Name: Nuclear factor of activated T-cells, cytoplasmic 1
• Protein Family: Nuclear factor of activated T-cells
• UniProt Gene Name: NFATC1
• UniProt Synonym Gene Names: NFAT2; NFATC; NF-ATc1; NFATc1; NF-ATc; NFATc
• UniProt Entry Name: NFAC1_HUMAN

NCBI Description

The product of this gene is a component of the nuclear factor of activated T cells DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation, and an inducible nuclear component. Proteins belonging to this family of transcription factors play a central role in inducible gene transcription during immune response. The product of this gene is an inducible nuclear component. It functions as a major molecular target for the immunosuppressive drugs such as cyclosporin A. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. Different isoforms of this protein may regulate inducible expression of different cytokine genes. [provided by RefSeq, Jul 2013]

Uniprot Description

NFAT2: a transcription factor that plays a role in the inducible expression of cytokine genes in T cells, especially in the induction of the IL-2 and IL-4. Also control gene expression in embryonic cardiac cells. Could regulate not only the activation and proliferation but also the differentiation and programmed death of lymphoid and nonlymphoid cells. Six splice variant isoforms have been identified. Expressed in thymus, peripheral leukocytes as T-cells and spleen. Isoforms A are preferentially expressed in effector T-cells (thymus and peripheral leukocytes) whereas isoforms B and isoforms C are preferentially expressed in naive T-cells (spleen). Isoforms B are expressed in naive T-cells after first antigen exposure and isoforms A are expressed in effector T-cells after second antigen exposure.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 18q23

Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; nuclear chromatin; nucleus; cytosol

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; mitogen-activated protein kinase p38 binding; FK506 binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; heart development; positive regulation of transcription, DNA-dependent; calcium ion transport; innate immune response; epithelial to mesenchymal transition; positive regulation of transcription from RNA polymerase II promoter; osteoclast differentiation; G1/S transition of mitotic cell cycle