Mouse CCAAT/enhancer-binding protein beta Monoclonal Antibody | anti-CEBPB antibody

Mouse Anti Human CCAAT/enhancer-binding protein beta

Cat. Num. AAA140227

• Gene Names: CEBPB; TCF5; IL6DBP; NF-IL6; C/EBP-beta
• Applications: ELISA, Western Blot
• Purity: CEBPB antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
• Synonyms: CCAAT/enhancer-binding protein beta; Monoclonal Antibody; Mouse Anti Human CCAAT/enhancer-binding protein beta; CEBPB Antibody; Mouse Anti Human; C/EBP beta; Liver activator protein; Nuclear factor NF-IL6; Transcription factor 5; TCF-5; CEBPB; LAP; TCF5; CRP2; IL6DBP; MGC32080; anti-CEBPB antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1 heavy chain and kappa light chain
• Clone Number: P47A1AT
• Purity/Purification: CEBPB antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
• Form/Format: 1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide.
• Sequence Length: 345

• Applicable Applications for anti-CEBPB antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: CEBPB antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 2,000. Recommended starting dilution is 1:1,000.
• Immunogen: Anti-human CEBPB mAb, is derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with recombinant human CEBPB amino acids 1-271 purified from E Coli.
• Preparation and Storage: For periods up to 1 month store at 4 degree C, for longer periods of time, store at -20 degree C. Prevent freeze thaw cycles.; 12 months at -20 degree C. 1 month at 4 degree C.

Related Product Information for anti-CEBPB antibody

Introduction: CEBPB is an intronless gene and its protein is a bZIP transcription factor which can bind as a homodimer to certain DNA regulatory regions. CEBPB can also form heterodimers with the related proteins CEBP-alpha, CEBP-delta, and CEBP-gamma. CEBPB is important in the regulation of genes involved in immune and inflammatory responses and has been shown to bind to the IL-1 response element in the IL-6 gene, as well as to regulatory regions of several acute-phase and cytokine genes. In addition, CEBPB can bind the promoter and upstream element and stimulate the expression of the collagen type I gene.

Product Categories/Family for anti-CEBPB antibody

Monoclonal/Polyclonal Antibodies; Monoclonal Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 28872796
• NCBI GeneID: 1051
• NCBI Accession #: NP_005185.2
• NCBI GenBank Nucleotide #: NM_005194.3
• Molecular Weight: 15,938 Da
• NCBI Official Full Name: CCAAT/enhancer-binding protein beta isoform a
• NCBI Official Synonym Full Names: CCAAT/enhancer binding protein (C/EBP), beta
• NCBI Official Symbol: CEBPB
• NCBI Official Synonym Symbols: TCF5; IL6DBP; NF-IL6; C/EBP-beta
• NCBI Protein Information: CCAAT/enhancer-binding protein beta; interleukin 6-dependent DNA-binding protein; nuclear factor NF-IL6; nuclear factor of interleukin 6; transcription factor 5
• UniProt Protein Name: CCAAT/enhancer-binding protein beta
• Protein Family: CCAAT/enhancer-binding protein
• UniProt Gene Name: CEBPB
• UniProt Synonym Gene Names: TCF5; C/EBP beta; LAP; LIP; TCF-5
• UniProt Entry Name: CEBPB_HUMAN

NCBI Description

This intronless gene encodes a transcription factor that contains a basic leucine zipper (bZIP) domain. The encoded protein functions as a homodimer but can also form heterodimers with CCAAT/enhancer-binding proteins alpha, delta, and gamma. Activity of this protein is important in the regulation of genes involved in immune and inflammatory responses, among other processes. The use of alternative in-frame AUG start codons results in multiple protein isoforms, each with distinct biological functions. [provided by RefSeq, Oct 2013]

Uniprot Description

C/EBP-beta: a bZIP transcription factor which can form homodimers or heterodimers with the related proteins CEBP-alpha, -delta and -gamma. Involved in immune and inflammatory responses. Specifically binds to regulatory regions of genes encoding IL-6, other cytokines and several acute-phase proteins. There are two forms of C/EBPbeta, the 38kDa liver activating protein (LAP) and the 20kDa liver inhibitory protein (LIP) which may be products of alternative translation. LAP is a transcriptional activator while LIP may inhibit C/EBPbeta transcriptional activity. Phosphorylated and activated by ERK1/2.

Protein type: Transcription factor; DNA-binding; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 20q13.1

Cellular Component: nucleoplasm; nuclear matrix; cytoplasm; nuclear chromatin; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; protein homodimerization activity; DNA binding; protein heterodimerization activity; chromatin binding; transcription factor binding; transcription factor activity; glucocorticoid receptor binding

Biological Process: transcription from RNA polymerase II promoter; embryonic placenta development; regulation of interleukin-6 biosynthetic process; mammary gland epithelial cell proliferation; response to lipopolysaccharide; neuron differentiation; positive regulation of osteoblast differentiation; regulation of transcription, DNA-dependent; brown fat cell differentiation; acute-phase response; positive regulation of fat cell differentiation; immune response; positive regulation of transcription from RNA polymerase II promoter; negative regulation of neuron apoptosis; inflammatory response; negative regulation of transcription, DNA-dependent