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Testing Data #1
Heparanase-1 3/17 Antibody | anti-HEP-1 3/17 antibody
Mouse Anti Human Heparanase-1 3/17
Applications
Western Blot, Immunohistochemistry
Purity
>98% on SDS-PAGE when loaded 50 ug/lane
Synonyms
Heparanase-1 3/17; Mouse Anti Human Heparanase-1 3/17; Heparanase 1 Clone HP3/17; Heparanase 1 (HPA1); InSight Monoclonal Anti-Human Antibody; anti-HEP-1 3/17 antibody
Isotype
Mouse IgG2B?
Specificity
HP3/17 reacts with the 50 kDa subunit and with the 65 kDa precursor of human or mouse Heparanase by Western blotting and immunohistochemistry. Recommended dilution range for Western blot analysis: 1:4000. Recommended dilution range for immunohistochemistry: 1:40.
Purity/Purification
>98% on SDS-PAGE when loaded 50 ug/lane
Form/Format
Each vial contains 50, 100 or 150 ug in 12.5, 25 or 37.5 ul respectively, of 0.22 micron filtered solution of 20 mM Sodium Phosphate; 150 mM NaCl; pH 7.2, containing 0.01% Thimerosal.
Applicable Applications for anti-HEP-1 3/17 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Source Note
Mab HP3/17 is a Protein G affinity purified monoclonal antibody raised against a polypeptide from the 50 kDa subunit of Heparanase.
Patents
Anti-heparanase antibodies and their uses, including HP3/17 and its uses, are protected by US. Patents No. 6,177,545; 6,531,129, additional US patent applications and patents and patent applications worldwide.
Preparation and Storage
Store at 4 degree C. Stable for six months from the date of shipment. For extended storage, freeze in working aliquots at -20 degree C. Avoid repeated freeze-thaw cycles.
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Related Product Information for anti-HEP-1 3/17 antibody
Heparanase is an endo-beta-D-glucuronidase, which degrades heparan sulfate side chains of heparan sulfate proteoglycans (HSPGs) in the extracellular matrix. Heparanase plays an important role in ECM degradation, facilitating the migration and extravasation of tumor cells and inflammatory leukocytes (1,2,3). Upon degradation, heparanase releases growth factors and cytokines that stimulate cell proliferation and chemotaxis (4,5).
Heparanase is a heterodimer comprised of a 50 kDa subunit harboring the active site and a 8 kDa subunit. It is produced as a latent 65 kDa precursor and proteolytically processed to its active form (1,6).
Heparanase is highly expressed in myeloid leukocytes (i.e. neutrophils) in platelets and in human placenta. Human heparanase was found to be upregulated in various types of primary tumors, correlating in some cases with increased tumor invasiveness and vascularity and with poor prospective survival (7,8).
Heparanase is a heterodimer comprised of a 50 kDa subunit harboring the active site and a 8 kDa subunit. It is produced as a latent 65 kDa precursor and proteolytically processed to its active form (1,6).
Heparanase is highly expressed in myeloid leukocytes (i.e. neutrophils) in platelets and in human placenta. Human heparanase was found to be upregulated in various types of primary tumors, correlating in some cases with increased tumor invasiveness and vascularity and with poor prospective survival (7,8).
Product Categories/Family for anti-HEP-1 3/17 antibody
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Product Notes
The HEP-1 3/17 (Catalog #AAA140055) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Heparanase-1 3/17 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Researchers should empirically determine the suitability of the HEP-1 3/17 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Heparanase-1 3/17, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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