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Gamma-F420-2:alpha-L-glutamate ligase (cofF) Recombinant Protein | cofF recombinant protein

Recombinant Methanocaldococcus jannaschii Gamma-F420-2:alpha-L-glutamate ligase (cofF)

Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Synonyms
Gamma-F420-2:alpha-L-glutamate ligase (cofF); Recombinant Methanocaldococcus jannaschii Gamma-F420-2:alpha-L-glutamate ligase (cofF); Gamma-F420-2:alpha-L-glutamate ligase; EC=6.3.2.-; cofF recombinant protein
Ordering
For Research Use Only!
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE.
Form/Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Sequence Positions
1-288aa; full length protein
Sequence
MVKITILSPE GRSCSVWSLK NEIEKLGAKC DIFLLSSPEN LMSHDFKLET DLIHSRCGIGDYFDRLTLYS WQFINALEVE GCRFINPIKT LYLTSDKFKC IKLLAKNKIK TPKTALIRDYEDAVKFIEKY NLRFPVVIKN SFSKCGLKVF MARNYDELKQ LTKNAIWEGK LIQEFIDFKENDLYRDMRIL VVDGEVVGGY RRVSRDFRTN LYLGNVVEKL NIDEELEELA LKCADLSEAVILGVDILPTK DNYYVIELNS SPGTKGFRDI GINADKKIAE ALVRYAKS
Sequence Length
288
Species
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Preparation and Storage
Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
33,112 Da
NCBI Official Full Name
30S ribosomal protein S6 modification protein RimK
NCBI Official Symbol
MJ_1001
NCBI Protein Information
30S ribosomal protein S6 modification protein RimK
UniProt Protein Name
Coenzyme gamma-F420-2:alpha-L-glutamate ligase
Protein Family
UniProt Gene Name
cofF
UniProt Entry Name
COFF_METJA

Uniprot Description

Catalyzes the ATP-dependent addition of one alpha-linked L-glutamate molecule to coenzyme gamma-F420-2, producing alpha-F420-3, the major form of coenzyme F420 found in M.jannaschii. Thus, caps the gamma-polyglutamate tail of coenzyme F420 with a terminal alpha-linked glutamate. Prefers ATP to other purine nucleotide triphosphates; GTP gives about 25% of the activity observed with ATP. Cannot catalyze the addition of the following amino acids or analogs: D-glutamate, beta-glutamate, L-aspartate, L-glutamine, L-alpha-aminoadipate, or D,L-2-amino-4-phosphono-butyrate.

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Product Notes

The cofF coff (Catalog #AAA1372602) is a Recombinant Protein produced from E Coli or Yeast or Baculovirus or Mammalian Cell and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-288aa; full length protein. The amino acid sequence is listed below: MVKITILSPE GRSCSVWSLK NEIEKLGAKC DIFLLSSPEN LMSHDFKLET DLIHSRCGIG DYFDRLTLYS WQFINALEVE GCRFINPIKT LYLTSDKFKC IKLLAKNKIK TPKTALIRDY EDAVKFIEKY NLRFPVVIKN SFSKCGLKVF MARNYDELKQ LTKNAIWEGK LIQEFIDFKE NDLYRDMRIL VVDGEVVGGY RRVSRDFRTN LYLGNVVEKL NIDEELEELA LKCADLSEAV ILGVDILPTK DNYYVIELNS SPGTKGFRDI GINADKKIAE ALVRYAKS. It is sometimes possible for the material contained within the vial of "Gamma-F420-2:alpha-L-glutamate ligase (cofF), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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