1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-4 Recombinant Protein | PLCD4 recombinant protein

Recombinant Human 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-4

Cat. Num. AAA1367818

• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: 1-phosphatidylinositol 4; 5-bisphosphate phosphodiesterase delta-4; Recombinant Human 1-phosphatidylinositol 4; Phosphoinositide phospholipase C-delta-4; Phospholipase C-delta-4; PLC-delta-4; PLCD4 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1-762aa; Full Length
• Sequence: MASLLQDQLTTDQDLLLMQEGMPMRKVRSKSWKKLRYFRLQNDGMTVWHARQARGSAKPSFSISDVETIRNGHDSELLRSLAEELPLEQGFTIVFHGRRSNLDLMANSVEEAQIWMRGLQLLVDLVTSMDHQERLDQWLSDWFQRGDKNQDGKMSFQEVQRLLHLMNVEMDQEYAFSLFQAADTSQSGTLEGEEFVQFYKALTKRAEVQELFESFSADGQKLTLLEFLDFLQEEQKERDCTSELALELIDRYEPSDSGKLRHVLSMDGFLSYLCSKDGDIFNPACLPIYQDMTQPLNHYFICSSHNTYLVGDQLCGQSSVEGYIRALKRGCRCVEVDVWDGPSGEPVVYHGHTLTSRILFKDVVATVAQYAFQTSDYPVILSLETHCSWEQQQTMARHLTEILGEQLLSTTLDGVLPTQLPSPEELRRKILVKGKKLTLEEDLEYEEEEAEPELEESELALESQFETEPEPQEQNLQNKDKKKKSKPILCPALSSLVIYLKSVSFRSFTHSKEHYHFYEISSFSETKAKRLIKEAGNEFVQHNTWQLSRVYPSGLRTDSSNYNPQELWNAGCQMVAMNMQTAGLEMDICDGHFRQNGGCGYVLKPDFLRDIQSSFHPEKPISPFKAQTLLIQVISGQQLPKVDKTKEGSIVDPLVKVQIFGVRLDTARQETNYVENNGFNPYWGQTLCFRVLVPELAMLRFVVMDYDWKSRNDFIGQYTLPWTCMQQGYRHIHLLSKDGISLRPASIFVYICIQEGLEGDES
• Sequence Length: 762

• Preparation and Storage: Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.

SDS-PAGE

Related Product Information for PLCD4 recombinant protein

Hydrolyzes the phosphatidylinositol 4,5-bisphosphate (PIP2) to generate 2 second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). DAG mediates the activation of protein kinase C (PKC), while IP3 releases Ca2+ from intracellular stores. Required for acrosome reaction in sperm during fertilization, probably by acting as an important enzyme for intracellular Ca2+ mobilization in the zona pellucida-induced acrosome reaction. May play a role in cell growth. Modulates the liver regeneration in cooperation with nuclear PKC. Overexpression up-regulates the Erk signaling pathway and proliferation.

Product Categories/Family for PLCD4 recombinant protein

Signal Transduction

References

Phospholipase C delta-4 overexpression upregulates ErbB1/2 expression, Erk signaling pathway, and proliferation in MCF-7 cells.Leung D.W., Tompkins C., Brewer J., Ball A., Coon M., Morris V., Waggoner D., Singer J.W.Mol. Cancer 3:15-15(2004) Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S. Assignment of the human PLC delta4 gene (PLCD4) to human chromosome band 2q35 by fluorescence in situ hybridization.Kim H., Suh P.-G., Ryu S.H., Park S.H.Cytogenet. Cell Genet. 87:254-255(1999) Expression of phospholipases A2 and C in human corneal epithelial cells.Landreville S., Coulombe S., Carrier P., Gelb M.H., Guerin S.L., Salesse C.Invest. Ophthalmol. Vis. Sci. 45:3997-4003(2004)

NCBI and Uniprot Product Information

• NCBI GI #: 14249340
• NCBI GeneID: 84812
• NCBI Accession #: NP_116115.1
• NCBI GenBank Nucleotide #: NM_032726.3
• UniProt Accession #: Q9BRC7; Q53FS8
• Molecular Weight: 103.6 kDa
• NCBI Official Full Name: 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-4
• NCBI Official Synonym Full Names: phospholipase C delta 4
• NCBI Official Symbol: PLCD4
• NCBI Protein Information: 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-4
• UniProt Protein Name: 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-4
• Protein Family: 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
• UniProt Gene Name: PLCD4
• UniProt Synonym Gene Names: hPLCD4; PLC-delta-4
• UniProt Entry Name: PLCD4_HUMAN

NCBI Description

This gene encodes a member of the delta class of phospholipase C enzymes. Phospholipase C enzymes play a critical role in many cellular processes by hydrolyzing phosphatidylinositol 4,5-bisphosphate into two intracellular second messengers, inositol 1,4,5-trisphosphate and diacylglycerol. Expression of this gene may be a marker for cancer. [provided by RefSeq, Jan 2011]

Uniprot Description

PLCD4: Hydrolyzes the phosphatidylinositol 4,5-bisphosphate (PIP2) to generate 2 second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). DAG mediates the activation of protein kinase C (PKC), while IP3 releases Ca(2+) from intracellular stores. Required for acrosome reaction in sperm during fertilization, probably by acting as an important enzyme for intracellular Ca(2+) mobilization in the zona pellucida- induced acrosome reaction. May play a role in cell growth. Modulates the liver regeneration in cooperation with nuclear PKC. Overexpression up-regulates the Erk signaling pathway and proliferation. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.4.11; Carbohydrate Metabolism - inositol phosphate; Phospholipase

Chromosomal Location of Human Ortholog: 2q35

Cellular Component: cytoplasm; endoplasmic reticulum; nuclear membrane; plasma membrane

Molecular Function: calcium ion binding; phosphoinositide phospholipase C activity; signal transducer activity

Biological Process: acrosome reaction; lipid catabolic process; phosphatidylinositol metabolic process