Mouse Prekallikrein ELISA Kit | KLKB1 elisa kit

Mouse Prekallikrein Total Antigen Assay ELISA kit

Cat. Num. AAA136095

• Gene Names: Klkb1; APS; PSA; Kal3; Klk3; Kal-3
• Reactivity: Mouse
• Synonyms: Prekallikrein; Mouse Prekallikrein Total Antigen Assay ELISA kit; KLKB1 elisa kit

• Reactivity: Mouse
• Specificity: This assay recognizes total mouse prekallikrein. Pooled normal plasma from rat, rabbit, dog, pig, sheep, horse, human, cyno monkey and rhesus monkey was assayed and no significant cross-reactivity was observed.
• Sequence Length: 638

• Samples: Mouse Plasma.
• Intra-assay Precision: Three samples of known concentration were tested twenty times on one plate to assess in tra ~ a5say precision
• Inter-assay Precision: These studies are currently in progress. Please contact us for more information.
• Preparation and Storage: Store at 4 degree C.

Related Product Information for KLKB1 elisa kit

Intended Uses: This mouse prekallikrein total antigen assay is intended for the quantitat ive determination of tota l prekal likrein antigen in mouse plasma. For research use only.

Principle of the Assay: Mouse prekallikrein will bind to t he monoclonal capture antibody coated on the micrptiter plate. Preka llikrein and kallikrein will react with the antibody on the plate. After appropriate washing steps, anti-mouse prekallikrein primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with horseradish peroxidase conjugated streptavidin. Following an additional washing step, TM B substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of mouse prekallikrein. Color development is proportional to the concentration of total prekallikrein in the samples!!Background/Introduction: Preka llikrein is the glycosylated single chain zymogen precursor of the plasma serine protease kall ikrein. Plasma prekallikrein circulates with kininogen and is activated by Factor Xlla in the intrinsic coagulation pathway. Kallikrein activates plasminogen in fibrinolysis and cleaves kininogen in the bradykinin system of vasodilation. Prekallikrein deficiency is rare and causes increased activated partial thromboplastin time [1]. Elevated plasma prekallikrein is associated with diabetes [2] and cardiovascular disease [3].

Product Categories/Family for KLKB1 elisa kit

Mouse Prekallikrein ELISA Kits; Mouse Kits

NCBI and Uniprot Product Information

• NCBI GI #: 236465805
• NCBI GeneID: 16621
• NCBI Accession #: NP_032481.2
• NCBI GenBank Nucleotide #: NM_008455.3
• UniProt Accession #: P26262; Q8R0P5
• Molecular Weight: 71,383 Da
• NCBI Official Full Name: plasma kallikrein preproprotein
• NCBI Official Synonym Full Names: kallikrein B, plasma 1
• NCBI Official Symbol: Klkb1
• NCBI Official Synonym Symbols: APS; PSA; Kal3; Klk3; Kal-3
• NCBI Protein Information: plasma kallikrein
• UniProt Protein Name: Plasma kallikrein
• Protein Family: Plasma kallikrein
• UniProt Gene Name: Klkb1
• UniProt Synonym Gene Names: Klk3; Pk

NCBI Description

This gene encodes a member of the kallikrein subfamily of serine proteases that are involved in diverse physiological functions such as skin desquamation, tooth enamel formation, seminal liquefaction, synaptic neural plasticity and brain function. The encoded preproprotein undergoes proteolytic processing to generate a disulfide-linked heterodimeric enzyme comprised of heavy and light chains. A complete deletion of the encoded protein prevents occlusive thrombus formation in mice with a minimal role in provoked bleeding. [provided by RefSeq, May 2016]

Uniprot Description

The enzyme cleaves Lys-Arg and Arg-Ser bonds. It activates, in a reciprocal reaction, factor XII after its binding to a negatively charged surface. It also releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin.