Mouse antiplasmin total ELISA Kit | Serpinf2 elisa kit
Mouse antiplasmin total antigen assay ELISA kit
Minimum Shelf Life: 12 months from manufacture, see label for expiration date
Shipping Condition: Cold pack
Principle of the Assay: MOGAT1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-MOGAT1 antibody and an MOGAT1-HRP conjugate. The assay sample and buffer are incubated together with MOGAT1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MOGAT1 concentration since MOGAT1 from samples and MOGAT1-HRP conjugate compete for the anti-MOGAT1 antibody binding site. Since the number of sites is limited, as more sites are occupied by MOGAT1 from the sample, fewer sites are left to bind MOGAT1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MOGAT1 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
Uniprot Description
SERPINF2: Serine protease inhibitor. The major targets of this inhibitor are plasmin and trypsin, but it also inactivates matriptase-3/TMPRSS7 and chymotrypsin. Defects in SERPINF2 are the cause of alpha-2-plasmin inhibitor deficiency (APLID). APLID is an autosomal recessive disorder resulting in severe hemorrhagic diathesis. Belongs to the serpin family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted; Secreted, signal peptide
Cellular Component: extracellular space; cell surface; fibrinogen complex; extracellular region
Molecular Function: serine-type endopeptidase inhibitor activity; protein homodimerization activity; protease binding; protease inhibitor activity
Biological Process: negative regulation of fibrinolysis; collagen fibril organization; renin-angiotensin regulation of blood vessel size; positive regulation of smooth muscle cell proliferation; positive regulation of stress fiber formation; positive regulation of collagen biosynthetic process; acute-phase response; positive regulation of JNK cascade; blood vessel morphogenesis; negative regulation of peptidase activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of cell differentiation
Disease: Alpha-2-plasmin Inhibitor Deficiency
Research Articles on Serpinf2
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Product Notes
The Mouse Serpinf2 serpinf2 (Catalog #AAA135940) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA135940 ELISA Kit recognizes Mouse Serpinf2. It is sometimes possible for the material contained within the vial of "antiplasmin total, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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