Prorenin, C-terminal 8x His tag Protein | REN protein

Human Prorenin, C-terminal 8x His tag

Cat. Num. AAA135823

• Gene Names: REN; HNFJ2
• Purity: >95% by SDS-PAGE analysis
• Synonyms: Prorenin; C-terminal 8x His tag; Human Prorenin; REN protein

• Host: Human Embryonic Kidney cells
• Purity/Purification: >95% by SDS-PAGE analysis
• Form/Format: Frozen liquid
• Concentration: 0.9 mg/ml (varies by lot)
• Sequence Length: 406

• Buffer: 50mM Tris; pH 8.0
• Extinction Coefficient: 1.1
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Store at -70 degree C. Shelf Life: 3 years from delivery

Related Product Information for REN protein

Recombinantly produced in HEK cell culture and purified by chelated metal affinity chromatography. Contains a 8X-Histidine tag at C terminus for purification. Fully activatable to renin by catalytic amounts of trypsin. Prorenin is a glycosylated aspartic protease that consists of 2 homologous lobes and is the precursor of renin. Prorenin exhibits a low level of enzymatic activity relative to renin which is generated from prorenin by proteolytic cleavage of the first ~43 amino acids at the N-terminus. This so called prosegment appears to block the full enzymatic potential of the active site (1). Renin activates the renin-angiotensin system by cleaving angiotensinogen, produced by the liver, to yield angiotensin I, which is further converted into angiotensin II by ACE, the angiotensin-converting enzyme primarily within the capillaries of the lungs. It has been reported that the levels of circulating prorenin (but not renin) are increased in diabetic subjects(2).

Product Categories/Family for REN protein

Renin/Prorenin; Human Prorenin

NCBI and Uniprot Product Information

• NCBI GI #: 4506475
• NCBI GeneID: 5972
• NCBI Accession #: NP_000528.1
• NCBI GenBank Nucleotide #: NM_000537.3
• UniProt Accession #: P00797; Q6FI38; Q6T5C2
• Molecular Weight: 43,725
• NCBI Official Full Name: renin preproprotein
• NCBI Official Synonym Full Names: renin
• NCBI Official Symbol: REN
• NCBI Official Synonym Symbols: HNFJ2
• NCBI Protein Information: renin; angiotensinogenase; renin precursor, renal; angiotensin-forming enzyme
• UniProt Protein Name: Renin
• Protein Family: Protein
• UniProt Gene Name: REN
• UniProt Entry Name: RENI_HUMAN

NCBI Description

Renin catalyzes the first step in the activation pathway of angiotensinogen--a cascade that can result in aldosterone release,vasoconstriction, and increase in blood pressure. Renin, an aspartyl protease, cleaves angiotensinogen to form angiotensin I, which is converted to angiotensin II by angiotensin I converting enzyme, an important regulator of blood pressure and electrolyte balance. Transcript variants that encode different protein isoforms and that arise from alternative splicing and the use of alternative promoters have been described, but their full-length nature has not been determined. Mutations in this gene have been shown to cause familial hyperproreninemia. [provided by RefSeq, Jul 2008]

Uniprot Description

REN: Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney. Interacts with ATP6AP2. Interaction with ATP6AP2 results in a 5-fold increased efficiency in angiotensinogen processing. Belongs to the peptidase A1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; EC 3.4.23.15; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 1q32

Cellular Component: extracellular space; cytoplasm; plasma membrane; extracellular region

Molecular Function: peptidase activity; insulin-like growth factor receptor binding; aspartic-type endopeptidase activity; receptor binding

Biological Process: response to cAMP; hormone-mediated signaling; male gonad development; cell maturation; renin-angiotensin regulation of aldosterone production; drinking behavior; response to lipopolysaccharide; proteolysis; angiotensin maturation; cellular protein metabolic process; regulation of MAPKKK cascade; beta-amyloid metabolic process; regulation of blood pressure; mesonephros development; kidney development

Disease: Renal Tubular Dysgenesis; Hyperuricemic Nephropathy, Familial Juvenile, 2