E3 ubiquitin-protein ligase TRIM21 (TRIM21) Recombinant Protein | TRIM21 recombinant protein

Recombinant Bovine E3 ubiquitin-protein ligase TRIM21 (TRIM21)

Cat. Num. AAA1347102

• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: E3 ubiquitin-protein ligase TRIM21 (TRIM21); Recombinant Bovine E3 ubiquitin-protein ligase TRIM21 (TRIM21); TRIM21 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1-469, full length protein
• Sequence: MASAVPLTMMWEEVTCSICLDPMVEPMSIECGHSFCQECISEVGKEGGSVCPVCRRHFLLQNLRPNRQVANMVDNLRKISQGAKESPHGELCVVHREKIHLFCEEDGKALCWVCSQSQKHRDHPMVPIEEAAQEYQEKLQVALNKLRHKQELAEKLELDIAMKKASWKGKVEAHKLRIHEEFVRQKNFLAEEEQKQLQKLEEEERQQLRTLGDTEARLAQQIQALQELIAELERRRRGSALELLQEVKSVLERSESWNLKELDVASTDLRNVFYVPGIKKMLRTCGVHITLDPQTANPWLILSENRRQVRLANTRQEVPENEERFDSYPMVLGAQRFDSGKVYWEVDVTGKEAWDLGVCRDNVRRKGQFLLNSDTGFWIIWLWNKQKYEAGTCPQTPLHLQVPPNRIGIFLDYEASTVSFYNITDHASLIYTFSECAFAGPLRPFFNPGFNDRGTNSAPLILCPLKTGW
• Sequence Length: 469

• Species: Bovine
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

Related Product Information for TRIM21 recombinant protein

This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The encoded protein is part of the RoSSA ribonucleoprotein, which includes a single polypeptide and one of four small RNA molecules. The RoSSA particle localizes to both the cytoplasm and the nucleus. RoSSA interacts with autoantigens in patients with Sjogren syndrome and systemic lupus erythematosus. Alternatively spliced transcript variants for this gene have been described but the full-length nature of only one has been determined.

NCBI and Uniprot Product Information

• NCBI GI #: 32880219
• NCBI GeneID: 359715
• NCBI Accession #: NP_872596.1
• NCBI GenBank Nucleotide #: NM_182655.2
• UniProt Accession #: Q7YRV4; A4FV39
• Molecular Weight: 54,031 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase TRIM21
• NCBI Official Synonym Full Names: tripartite motif containing 21
• NCBI Official Symbol: TRIM21
• NCBI Protein Information: E3 ubiquitin-protein ligase TRIM21
• UniProt Protein Name: E3 ubiquitin-protein ligase TRIM21
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: TRIM21
• UniProt Synonym Gene Names: SS-A

Uniprot Description

E3 ubiquitin-protein ligase whose activity is dependent on E2 enzymes, UBE2D1, UBE2D2, UBE2E1 and UBE2E2. Forms a ubiquitin ligase complex in cooperation with the E2 UBE2D2 that is used not only for the ubiquitination of USP4 and IKBKB but also for its self-ubiquitination. Component of cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes such as SCF(SKP2)-like complexes. A TRIM21-containing SCF(SKP2)-like complex is shown to mediate ubiquitination of CDKN1B ('Thr-187' phosphorylated-form), thereby promoting its degradation by the proteasome. Monoubiquitinates IKBKB that will negatively regulates Tax-induced NF-kappa-B signaling. Negatively regulates IFN-beta production post-pathogen recognition by polyubiquitin-mediated degradation of IRF3. Mediates the ubiquitin-mediated proteasomal degradation of IgG1 heavy chain, which is linked to the VCP-mediated ER-associated degradation (ERAD) pathway. Promotes IRF8 ubiquitination, which enhanced the ability of IRF8 to stimulate cytokine genes transcription in macrophages. Plays a role in the regulation of the cell cycle progression. Enhances the decapping activity of DCP2. Exists as a ribonucleoprotein particle present in all mammalian cells studied and composed of a single polypeptide and one of four small RNA molecules. At least two isoforms are present in nucleated and red blood cells, and tissue specific differences in RO/SSA proteins have been identified. The common feature of these proteins is their ability to bind HY RNAs.2. Involved in the regulation of innate immunity and the inflammatory response in response to IFNG/IFN-gamma. Organizes autophagic machinery by serving as a platform for the assembly of ULK1, Beclin 1/BECN1 and ATG8 family members and recognizes specific autophagy targets, thus coordinating target recognition with assembly of the autophagic apparatus and initiation of autophagy. Acts as an autophagy receptor for the degradation of IRF3, hence attenuating type I interferon (IFN)-dependent immune responses.