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RuvB-like 2 (RUVBL2) Recombinant Protein | RUVBL2 recombinant protein
Recombinant Human RuvB-like 2 (RUVBL2)
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Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Component of a SWR1-like complex that specifically mediates the removal of histone H2A.Z/H2AFZ from the nucleosome.
Proposed core component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair.
Plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex. May also inhibit the transcriptional activity of ATF2.
Involved in the endoplasmic reticulum (ER)-associated degradation (ERAD) pathway where it negatively regulates expression of ER stress response genes.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes the second human homologue of the bacterial RuvB gene. Bacterial RuvB protein is a DNA helicase essential for homologous recombination and DNA double-strand break repair. Functional analysis showed that this gene product has both ATPase and DNA helicase activities. This gene is physically linked to the CGB/LHB gene cluster on chromosome 19q13.3, and is very close (55 nt) to the LHB gene, in the opposite orientation. [provided by RefSeq, Jul 2008]
Uniprot Description
RUVBL2: Possesses single-stranded DNA-stimulated ATPase and ATP- dependent DNA helicase (5' to 3') activity; hexamerization is thought to be critical for ATP hydrolysis and adjacent subunits in the ring-like structure contribute to the ATPase activity. Forms homohexameric rings (Probable). Can form a dodecamer with RUVBL1 made of two stacked hexameric rings; however, even though RUVBL1 and RUVBL2 are present in equimolar ratio, the oligomeric status of each hexamer is not known. Oligomerization may regulate binding to nucleic acids and conversely, binding to nucleic acids may affect the dodecameric assembly. Interacts with the transcriptional activation domain of MYC. Interacts With ATF2. Component of the RNA polymerase II holoenzyme complex. May also act to bridge the LEF1/TCF1-CTNNB1 complex and TBP. Component of the NuA4 histone acetyltransferase complex which contains the catalytic subunit KAT5/TIP60 and the subunits EP400, TRRAP/PAF400, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, ING3, actin, ACTL6A/BAF53A, MORF4L1/MRG15, MORF4L2/MRGX, MRGBP, YEATS4/GAS41, VPS72/YL1 and MEAF6. The NuA4 complex interacts with MYC and the adenovirus E1A protein. RUVBL2 interacts with EP400. Component of a NuA4-related complex which contains EP400, TRRAP/PAF400, SRCAP, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, actin, ACTL6A/BAF53A, VPS72 and YEATS4/GAS41. Interacts with NPAT. Component of the chromatin- remodeling INO80 complex; specifically part of a complex module associated with the helicase ATP-binding and the helicase C- terminal domain of INO80. Component of some MLL1/MLL complex, at least composed of the core components MLL, ASH2L, HCFC1/HCF1, WDR5 and RBBP5, as well as the facultative components C17orf49, CHD8, E2F6, HSP70, INO80C, KANSL1, LAS1L, MAX, MCRS1, MGA, MYST1/MOF, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. Interacts with IGHMBP2. Interacts with TELO2. Ubiquitously expressed. Highly expressed in testis and thymus. Belongs to the RuvB family.
Protein type: Helicase; EC 3.6.4.12
Chromosomal Location of Human Ortholog: 19q13.3
Cellular Component: nucleoplasm; NuA4 histone acetyltransferase complex; nuclear matrix; membrane; cytoplasm; intracellular; ribonucleoprotein complex; nucleus
Molecular Function: ATP-dependent DNA helicase activity; identical protein binding; DNA helicase activity; protein binding; chromatin DNA binding; ATP-dependent 5'-3' DNA helicase activity; ATPase activity; unfolded protein binding; damaged DNA binding; ATP binding
Biological Process: chromatin remodeling; establishment and/or maintenance of chromatin architecture; protein folding; transcription, DNA-dependent; regulation of growth; positive regulation of histone acetylation; positive regulation of transcription from RNA polymerase II promoter; DNA repair; DNA duplex unwinding; DNA recombination
Research Articles on RUVBL2
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Product Notes
The RUVBL2 ruvbl2 (Catalog #AAA1342215) is a Recombinant Protein produced from E Coli or Yeast or Baculovirus or Mammalian Cell and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 2-463aa; Full Length of Mature Protein. The amino acid sequence is listed below: ATVTATTKVP EIRDVTRIER IGAHSHIRGL GLDDALEPRQ ASQGMVGQLA ARRAAGVVLE MIREGKIAGR AVLIAGQPGT GKTAIAMGMA QALGPDTPFT AIAGSEIFSL EMSKTEALTQ AFRRSIGVRI KEETEIIEGE VVEIQIDRPA TGTGSKVGKL TLKTTEMETI YDLGTKMIES LTKDKVQAGD VITIDKATGK ISKLGRSFTR ARDYDAMGSQ TKFVQCPDGE LQKRKEVVHT VSLHEIDVIN SRTQGFLALF SGDTGEIKSE VREQINAKVA EWREEGKAEI IPGVLFIDEV HMLDIESFSF LNRALESDMA PVLIMATNRG ITRIRGTSYQ SPHGIPIDLL DRLLIVSTTP YSEKDTKQIL RIRCEEEDVE MSEDAYTVLT RIGLETSLRY AIQLITAASL VCRKRKGTEV QVDDIKRVYS LFLDESRSTQ YMKEYQDAFL FNELKGETMD TS. It is sometimes possible for the material contained within the vial of "RuvB-like 2 (RUVBL2), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
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