Enterobactin synthase component E (entE) Recombinant Protein | entE recombinant protein
Recombinant Escherichia coli O157:H7 Enterobactin synthase component E (entE)
NCBI and Uniprot Product Information
Uniprot Description
Involved in the biosynthesis of the siderophore enterobactin (enterochelin), which is a macrocyclic trimeric lactone of N-(2,3-dihydroxybenzoyl)-serine. The serine trilactone serves as a scaffolding for the three catechol functionalities that provide hexadentate coordination for the tightly ligated iron2+ atoms. EntE proccesses via a two-step adenylation-ligation reaction (bi-uni-uni-bi ping-pong mechanism). First, it catalyzes the activation of the carboxylate group of 2,3-dihydroxy-benzoate (DHB), via a reversible ATP-dependent pyrophosphate exchange reactions to yield the acyladenylate intermediate 2,3-dihydroxybenzoyl-AMP. It can also transfer AMP to salicylate, 2,4-dihydroxybenzoate, gentisate and 2,3,4-trihydroxybenzoate. In the second step, DHB is transferred from 2,3-dihydroxybenzoyl-AMP onto the phosphopantetheinylated EntB (holo-EntB) to form DHB-holo-EntB. Then this product will serve in the formation of the amide bond between 2,3-dihydroxybenzoate (DHB) and L-serine.
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Product Notes
The entE ente (Catalog #AAA1338139) is a Recombinant Protein produced from E Coli or Yeast or Baculovirus or Mammalian Cell and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-536, Full length protein. The amino acid sequence is listed below: MSIPFTRWPE EFARRYREKG YWQDLPLTDI LTRHAASDSI AVIDGERQLS YRELNQAADN LACSLRRQGI KPGETALVQL GNVAELYITF FALLKLGVAP VLALFSHQRS ELNAYASQIE PALLIADRQH ALFSGDDFLN TFVAEHSSIR VVQLLNDSGE HNLQDAINHP ADGFTATPSP ADEVVYFQLS GGTTGTPKLI PRTHNDYYYS VRRSVEICQF TQQTRYLCAI PAAHNYAMSS PGSLGVFLAG GTVVLAADPS ATLCFPLIEK HQINVTALVP PAVSLWLQAL TEGESRAQLA SLKLLQVGGA RLSATLAARI PAEIGCQLQQ VFGMAEGLVN YTRLDDSAEK IIHTQGYPMC PDDEVWVADA EGNPLPQGEV GRLMTRGPYT FRGYYKSPQH NASAFDANGF YCSGDLISID PEGYITVQGR EKDQINRGGE KIAAEEIENL LLRHPAVIYA ALVSMEDELM GEKSCAYLVV KEPLRAVQVR RFLREQGIAE FKLPDRVECV DSLPLTAVGK VDKKQLRQWL ASRASA. It is sometimes possible for the material contained within the vial of "Enterobactin synthase component E (entE), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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