Homoaconitase large subunit (hacA) Recombinant Protein | hacA recombinant protein
Recombinant Methanocaldococcus jannaschii Homoaconitase large subunit (hacA)
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Synonyms
Homoaconitase large subunit (hacA); Recombinant Methanocaldococcus jannaschii Homoaconitase large subunit (hacA); Homoaconitase large subunit; HACN; EC=4.2.1.36; (R)-homocitrate dehydratase; EC=4.2.1.-; Homoaconitate hydratase; Methanogen homoaconitase; EC=4.2.1.114; hacA recombinant protein
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE.
Form/Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Sequence Positions
1-420aa; full length protein
Sequence
MTLVEKILSK KVGYEVCAGD SIEVEVDLAM THDGTTPLAY KALKEMSDSV WNPDKIVVAFDHNVPPNTVK AAEMQKLALE FVKRFGIKNF HKGGEGICHQ ILAENYVLPN MFVAGGDSHTCTHGAFGAFA TGFGATDMAY IYATGETWIK VPKTIRVDIV GKNENVSAKD IVLRVCKEIGRRGATYMAIE YGGEVVKNMD MDGRLTLCNM AIEMGGKTGV IEADEITYDY LKKERGLSDEDIAKLKKERI TVNRDEANYY KEIEIDITDM EEQVAVPHHP DNVKPISDVE GTEINQVFIGSCTNGRLSDL REAAKYLKGR EVHKDVKLIV IPASKKVFLQ ALKEGIIDIF VKAGAMICTPGCGPCLGAHQ GVLAEGEICL STTNRNFKGR MGHINSYIYL ASPKIAAISA VKGYITNKLD
Sequence Length
420
Species
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Preparation and Storage
Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
46,063 Da
NCBI Official Full Name
3-isopropylmalate dehydratase LeuC
NCBI Official Symbol
MJ_1003
NCBI Protein Information
3-isopropylmalate dehydratase LeuC
UniProt Protein Name
Methanogen homoaconitase large subunit
Protein Family
UniProt Gene Name
hacA
UniProt Synonym Gene Names
HACN
UniProt Entry Name
HACA_METJA
Uniprot Description
Hydro-lyase with broad substrate specificity for cis-unsaturated tricarboxylic acids. Catalyzes both the reversible dehydration of (R)-homocitrate ((R)-2-hydroxybutane-1,2,4-tricarboxylate) to produce cis-homoaconitate ((Z)-but-1-ene-1,2,4-tricarboxylate), and its hydration to homoisocitrate ((1R,2S)-1-hydroxybutane-1,2,4-tricarboxylate). Is also able to hydrate the analogous longer chain substrates cis-homo(2)-aconitate, cis-homo(3)-aconitate, and even the non-physiological cis-homo(4)-aconitate with similar efficiency. These reactions are part of the biosynthesis pathway of coenzyme B. Can also catalyze the hydration of maleate to (R)-malate, and that of cis-aconitate. Can not catalyze the hydration of citraconate and the dehydration of (S)-homocitrate, citramalate, 2-isopropylmalate, 3-isopropylmalate, citrate or threo-DL-isocitrate.
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Product Notes
The hacA haca (Catalog #AAA1326034) is a Recombinant Protein produced from E Coli or Yeast or Baculovirus or Mammalian Cell and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-420aa; full length protein. The amino acid sequence is listed below: MTLVEKILSK KVGYEVCAGD SIEVEVDLAM THDGTTPLAY KALKEMSDSV WNPDKIVVAF DHNVPPNTVK AAEMQKLALE FVKRFGIKNF HKGGEGICHQ ILAENYVLPN MFVAGGDSHT CTHGAFGAFA TGFGATDMAY IYATGETWIK VPKTIRVDIV GKNENVSAKD IVLRVCKEIG RRGATYMAIE YGGEVVKNMD MDGRLTLCNM AIEMGGKTGV IEADEITYDY LKKERGLSDE DIAKLKKERI TVNRDEANYY KEIEIDITDM EEQVAVPHHP DNVKPISDVE GTEINQVFIG SCTNGRLSDL REAAKYLKGR EVHKDVKLIV IPASKKVFLQ ALKEGIIDIF VKAGAMICTP GCGPCLGAHQ GVLAEGEICL STTNRNFKGR MGHINSYIYL ASPKIAAISA VKGYITNKLD. It is sometimes possible for the material contained within the vial of "Homoaconitase large subunit (hacA), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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