Lysine-specific demethylase REF6 (REF6) Recombinant Protein | REF6 recombinant protein

Recombinant Arabidopsis thaliana Lysine-specific demethylase REF6 (REF6) , partial

Cat. Num. AAA1322612

• Gene Names: REF6; JMJ12; Jumonji domain-containing protein 12; relative of early flowering 6
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Lysine-specific demethylase REF6 (REF6); Recombinant Arabidopsis thaliana Lysine-specific demethylase REF6 (REF6); partial; REF6 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 17-372. Partial
• Sequence: LPVAPEFRPTLAEFQDPIAYILKIEEEASRYGICKILPPLPPPSKKTSISNLNRSLAARAAARVRDGGFGACDYDGGPTFATRQQQIGFCPRKQRPVQRPVWQSGEEYSFGEFEFKAKNFEKNYLKKCGKKSQLSALEIETLYWRATVDKPFSVEYANDMPGSAFIPLSLAAARRRESGGEGGTVGETAWNMRAMSRAEGSLLKFMKEEIPGVTSPMVYVAMMFSWFAWHVEDHDLHSLNYLHMGAGKTWYGVPKDAALAFEEVVRVHGYGEELNPLVTFSTLGEKTTVMSPEVFVKAGIPCCRLVQNPGEFVVTFPGAYHSGFSHGFNFGEASNIATPEWLRMAKDAAIRRAAIN
• Sequence Length: 372

• Species: Arabidopsis thaliana (Mouse-ear cress)
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

NCBI and Uniprot Product Information

• NCBI GI #: 79586698
• NCBI GeneID: 824002
• NCBI Accession #: NP_680116.2
• NCBI GenBank Nucleotide #: NM_148863.4
• UniProt Accession #: Q9STM3
• Molecular Weight: 152,629 Da
• NCBI Official Full Name: relative of early flowering 6
• NCBI Official Symbol: REF6
• NCBI Official Synonym Symbols: JMJ12; Jumonji domain-containing protein 12; relative of early flowering 6
• NCBI Protein Information: relative of early flowering 6
• UniProt Protein Name: Lysine-specific demethylase REF6
• Protein Family: Lysine-specific demethylase
• UniProt Gene Name: REF6

NCBI Description

Relative of Early Flowering 6 (REF6) encodes a Jumonji N/C and zinc finger domain-containing protein that acts as a positive regulator of flowering in an FLC-dependent pathway. REF6 mutants have hyperacetylation of histone H4 at the FLC locus. REF6 interacts with BES1 in a Y2H assay and in vitro. REF6 may play a role in brassinoteroid signaling by affecting histone methylation in the promoters of BR-responsive genes. It is most closely related to the JHDM3 subfamily of JmjN/C proteins.

Uniprot Description

Histone demethylase that demethylates 'Lys-27' (H3K27me) of histone H3. Demethylates both tri- (H3K27me3) and di-methylated (H3K27me2) H3K27me (PubMed:21642989, PubMed:27111035). Demethylates also H3K4me3/2 and H3K36me3/2 in an in vitro assay (PubMed:20711170). Involved in the transcriptional regulation of hundreds of genes regulating developmental patterning and responses to various stimuli (PubMed:18467490). Binds DNA via its four zinc fingers in a sequence-specific manner, 5'-CTCTGYTY-3', to promote the demethylation of H3K27me3 and the regulation of organ boundary formation (PubMed:27111034, PubMed:27111035). Involved in the regulation of flowering time by repressing FLOWERING LOCUS C (FLC) expression (PubMed:15377760). Interacts with the NF-Y complexe to regulate SOC1 (PubMed:25105952). Mediates the recruitment of BRM to its target loci (PubMed:27111034).