RNA polymerase II-associated factor 1 homolog (Paf1) Recombinant Protein | Paf1 recombinant protein

Recombinant Rat RNA polymerase II-associated factor 1 homolog (Paf1)

Cat. Num. AAA1315754

• Gene Names: Paf1; RGD1306219
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: RNA polymerase II-associated factor 1 homolog (Paf1); Recombinant Rat RNA polymerase II-associated factor 1 homolog (Paf1); Paf1 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1-535, full length protein
• Sequence: MAPTIQTQAQREDGHRPNSHRTLPERSGVVCRVKYCNSLPDIPFDPKFITYPFDQNRFVQYKATSLEKQHKHDLLTEPDLGVTIDLINPDTYRIDPNVLLDPADEKLLEEEIQAPTSSKRSQQHAKVVPWMRKTEYISTEFNRYGISNEKPEVKIGVSVKQQFTEEEIYKDRDSQITAIEKTFEDAQKSISQHYSKPRVTPVEVMPVFPDFKMWINPCAQVIFDSDPAPKDTSGAAALEMMSQAMIRGMMDEEGNQFVAYFLPVEETLKKRKRDQEEEMDYAPDDVYDYKIAREYNWNVKNKASKGYEENYFFIFREGDGVYYNELETRVRLSKRRAKAGVQSGTNALLVVKHRDMNEKELEAQEARKAQLENHEPEEEEEEEMEAEEKEAGGSDEEHEKGSSSEKEGSEDERSGSESDREEGDRDEASDKSGSGEDESSEDEARAARDKEEIFGSDADSEDDADSDDEDRGQAHRGSDNDSDSGSDGGGQRSRSQSRSRSRSASPFPSGSEHSAQEDGSEAAASDSSEADSDSD
• Sequence Length: 535

• Species: Rat
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

NCBI and Uniprot Product Information

• NCBI GI #: 67846072
• NCBI GeneID: 361531
• NCBI Accession #: NP_001020069.1
• NCBI GenBank Nucleotide #: NM_001024898.1
• UniProt Accession #: Q4V886
• Molecular Weight: 60,546 Da
• NCBI Official Full Name: RNA polymerase II-associated factor 1 homolog
• NCBI Official Synonym Full Names: PAF1 homolog, Paf1/RNA polymerase II complex component
• NCBI Official Symbol: Paf1
• NCBI Official Synonym Symbols: RGD1306219
• NCBI Protein Information: RNA polymerase II-associated factor 1 homolog
• UniProt Protein Name: RNA polymerase II-associated factor 1 homolog
• Protein Family: RNA polymerase II-associated factor
• UniProt Gene Name: Paf1

Uniprot Description

Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non-phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both indepentently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of KMT2A/MLL1. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. Connects PAF1C with the RNF20/40 E3 ubiquitin-protein ligase complex. Involved in polyadenylation of mRNA precursors ().