Protein piwi (piwi) Recombinant Protein | piwi recombinant protein

Recombinant Drosophila melanogaster Protein piwi (piwi), partial

• Gene Names: piwi; CG6122; DmelCG6122; Piwi; PIWI
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Protein piwi (piwi); Recombinant Drosophila melanogaster Protein piwi (piwi); partial; piwi recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Length: 843

• Species: Drosophila melanogaster (Fruit fly)
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

NCBI and Uniprot Product Information

• NCBI GI #: 665407544
• NCBI GeneID: 34521
• NCBI Accession #: NP_001285825.1
• NCBI GenBank Nucleotide #: NM_001298896.1
• UniProt Accession #: Q9VKM1; C0PTU6; K7WKS7; K7WKT2; K7WQ39; K7WS94; K7XHZ2; L0CPR8; L0CPS4; L0CQ04; L0CR36; L0CRH9
• Molecular Weight: 97,177 Da
• NCBI Official Full Name: P-element induced wimpy testis, isoform B
• NCBI Official Synonym Full Names: P-element induced wimpy testis
• NCBI Official Symbol: piwi
• NCBI Official Synonym Symbols: CG6122; DmelCG6122; Piwi; PIWI
• NCBI Protein Information: CG6122 gene product from transcript CG6122-RA
• UniProt Protein Name: Protein piwi
• Protein Family: Protein
• UniProt Gene Name: piwi

Uniprot Description

Acts via the piwi-interacting RNA (piRNA) metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. In ovarian somatic cells, mediates silencing of transposable elements at the transcriptional level in a mael-dependent manner. In germ cells, mediates silencing at both transcriptional and post-transcriptional levels and is involved in the maintenance of populations of primary and secondary piRNAs. Piwi-mediated transcriptional silencing is accompanied by the formation of His3 'Lys-9' trimethylated (H3K9me3) euchromatin and heterochromatin. In ovary, associates predominantly with antisense piRNAs that contain uridine at their 5' end. Association with sense piRNAs is also observed but to a lesser extent. Involved in silencing of long terminal repeat (LTR) retrotransposons in male germline. Mediates a somatic signaling mechanism required for the maintenance of germline stem cells to produce and maintain a daughter germline stem cell. It is not essential for the further differentiation of the committed daughter cell. Acts cell autonomously to promote germline stem cell division. Its role in stem cell maintenance does not seem to require nuclear localization. Required maternally for the posterior localization of osk and vas and for pole cell formation during oogenesis and early embryogenesis. Together with Hop and Hsp83, mediates canalization, also known as developmental robustness, likely via epigenetic silencing of existing genetic variants and suppression of transposon-induced new genetic variation. Shows RNA cleavage activity, although is not required for any of its known functions.