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Western Blot (WB) (Western blot analysis of extracts of various cell lines, using UQCRC2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.)

Rabbit UQCRC2 Polyclonal Antibody | anti-UQCRC2 antibody

UQCRC2 Polyclonal Antibody

Gene Names
UQCRC2; QCR2; UQCR2; MC3DN5
Applications
Western Blot, Immunohistochemistry, Immunoprecipitation
Purity
Affinity Purification
Synonyms
UQCRC2; Polyclonal Antibody; UQCRC2 Polyclonal Antibody; QCR2; UQCR2; MC3DN5; anti-UQCRC2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Form/Format
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Sequence Length
453
Applicable Applications for anti-UQCRC2 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
Application Notes
WB: 1:500 - 1:2000
IHC: 1:50 - 1:100
IP: 1:50 - 1:200
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 224-453 of human UQCRC2 (NP_003357.2).
Cross Reactivity
Human, Mouse, Rat
Preparation and Storage
Store at -20°. Avoid freeze / thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using UQCRC2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.)

Western Blot (WB) (Western blot analysis of extracts of various cell lines, using UQCRC2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human liver injury using UQCRC2 antibody at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human liver injury using UQCRC2 antibody at dilution of 1:100 (40x lens).)

Immunoprecipitation (IP)

(Immunoprecipitation analysis of 200ug extracts of HepG2 cells using 1ug UQCRC2 antibody. Western blot was performed from the immunoprecipitate using UQCRC2 antibody at a dilition of 1:1000.)

Immunoprecipitation (IP) (Immunoprecipitation analysis of 200ug extracts of HepG2 cells using 1ug UQCRC2 antibody. Western blot was performed from the immunoprecipitate using UQCRC2 antibody at a dilition of 1:1000.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human colon using UQCRC2 Rabbit pAb (MBS129218) at dilution of 1:100(40x lens).)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human colon using UQCRC2 Rabbit pAb (MBS129218) at dilution of 1:100(40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded rat kidney using UQCRC2 Rabbit pAb (MBS129218) at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded rat kidney using UQCRC2 Rabbit pAb (MBS129218) at dilution of 1:100 (40x lens).)
Product Categories/Family for anti-UQCRC2 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
Observed MW: 48kDa
Calculated MW: 48 kDa
NCBI Official Full Name
Cytochrome b-c1 complex subunit 2, mitochondrial
NCBI Official Synonym Full Names
ubiquinol-cytochrome c reductase core protein II
NCBI Official Symbol
UQCRC2
NCBI Official Synonym Symbols
QCR2; UQCR2; MC3DN5
NCBI Protein Information
cytochrome b-c1 complex subunit 2, mitochondrial; core protein II; complex III subunit 2; ubiquinol-cytochrome-c reductase complex core protein 2
UniProt Protein Name
Cytochrome b-c1 complex subunit 2, mitochondrial
UniProt Gene Name
UQCRC2
UniProt Entry Name
QCR2_HUMAN

NCBI Description

The protein encoded by this gene is located in the mitochondrion, where it is part of the ubiquinol-cytochrome c reductase complex (also known as complex III). This complex constitutes a part of the mitochondrial respiratory chain. Defects in this gene are a cause of mitochondrial complex III deficiency nuclear type 5. [provided by RefSeq, Jul 2015]

Uniprot Description

UQCRC2: This is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain. The core protein 2 is required for the assembly of the complex. Belongs to the peptidase M16 family. UQCRC2/QCR2 subfamily.

Protein type: EC 1.10.2.2; Mitochondrial; Oxidoreductase; Energy Metabolism - oxidative phosphorylation

Chromosomal Location of Human Ortholog: 16p12

Cellular Component: mitochondrion; mitochondrial inner membrane; mitochondrial respiratory chain complex III

Molecular Function: protein binding; metal ion binding; metalloendopeptidase activity; protein complex binding

Biological Process: cellular metabolic process; aerobic respiration; proteolysis; oxidative phosphorylation

Disease: Mitochondrial Complex Iii Deficiency, Nuclear Type 5

Research Articles on UQCRC2

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Product Notes

The UQCRC2 uqcrc2 (Catalog #AAA129218) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's UQCRC2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP). WB: 1:500 - 1:2000 IHC: 1:50 - 1:100 IP: 1:50 - 1:200. Researchers should empirically determine the suitability of the UQCRC2 uqcrc2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "UQCRC2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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