Rabbit anti-Human, Mouse CTBP1 Polyclonal Antibody | anti-CTBP1 antibody

CTBP1 Polyclonal Antibody

Cat. Num. AAA127866

• Gene Names: CTBP1; BARS
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Immunoprecipitation
• Purity: Affinity Purification
• Synonyms: CTBP1; Polyclonal Antibody; CTBP1 Polyclonal Antibody; BARS; MGC104684; anti-CTBP1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 440

• Applicable Applications for anti-CTBP1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), CHIP
• Application Notes: WB: 1:500 - 1:2000; IHC: 1:50 - 1:200; IF: 1:50 - 1:200; IP: 1:50 - 1:200; ChIP: 1:20 - 1:100
• Species: Human
• Immunogen: Recombinant Protein
• Immunogen: Recombinant protein of human CTBP1
• Calculated Molecular Weight: 48kDa
• Preparation and Storage: Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.; Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Western Blot (WB)

(Western blot analysis of extracts of mouse brain, using CTBP1 Antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human liver cancer using CTBP1 antibody at dilution of 1:100 (40x lens).)

Related Product Information for anti-CTBP1 antibody

CtBP1 (C-terminal binding protein 1) was first recognized as a cellular factor that interacts with the C-terminal portion of adenovirus E1A, a protein involved in the transcriptional regulation of key cellular genes (1). CtBP1 is able to regulate gene activity through its intrinsic dehydrogenase activity (2,3) and by interacting with Polycomb Group (PcG) proteins during development (4). Along with its homologue, CtBP2, it acts as a transcriptional corepressor of zinc-finger homeodomain factor deltaEF1 to regulate a wide range of cellular processes through transrepression mechanisms (5). Through its direct interaction with PRDM16, CtBP1 has been shown to be involved in brown adipose tissue differentiation by mediating the repression of white fat genes and directing differentiation toward the brown fat gene program (6). CtBP1 also plays a role in lipid metabolic pathways and membrane fission by regulating the fission machinery operating Golgi tubular networks (7). CtBP1 has recently been shown to repress transcription of BRCA1 via a redox regulated mechanism (8). Furthermore, it is thought that downregulation of BRCA1 and E-cadherin in invasive ductal breast carcinoma correlates directly with activation of CtBP1 (9).

Product Categories/Family for anti-CTBP1 antibody

Polyclonal

NCBI and Uniprot Product Information

• NCBI GI #: 6014741
• NCBI GeneID: 1487
• UniProt Accession #: Q13363; Q4W5N3; Q7Z2Q5
• Molecular Weight: 440
• NCBI Official Full Name: C-terminal-binding protein 1
• NCBI Official Synonym Full Names: C-terminal binding protein 1
• NCBI Official Symbol: CTBP1
• NCBI Official Synonym Symbols: BARS
• NCBI Protein Information: C-terminal-binding protein 1; brefeldin A-ribosylated substrate
• UniProt Protein Name: C-terminal-binding protein 1
• UniProt Gene Name: CTBP1
• UniProt Synonym Gene Names: CTBP; CtBP1
• UniProt Entry Name: CTBP1_HUMAN

NCBI Description

This gene encodes a protein that binds to the C-terminus of adenovirus E1A proteins. This phosphoprotein is a transcriptional repressor and may play a role during cellular proliferation. This protein and the product of a second closely related gene, CTBP2, can dimerize. Both proteins can also interact with a polycomb group protein complex which participates in regulation of gene expression during development. Alternative splicing of transcripts from this gene results in multiple transcript variants. [provided by RefSeq, Jul 2008]

Uniprot Description

CTBP1: Involved in controlling the equilibrium between tubular and stacked structures in the Golgi complex. Functions in brown adipose tissue (BAT) differentiation. Corepressor targeting diverse transcription regulators such as GLIS2. Has dehydrogenase activity. Homo- or heterodimer. Heterodimer with CTBP2. Interacts with PRDM16; the interaction represses white adipose tissue (WAT)- specific genes expression. Interacts with GLIS2, FOXP2, HDAC4, HDAC5, HDAC9 and ZNF217. Interacts with adenovirus E1A protein (via its C-terminus); the interaction disrupts the interaction of CTBP1 with RBBP8. Interacts with Epstein-Barr virus EBNA3 and EBNA6. Interacts with ELK3 (via its PXDLS motif). Interacts with RBBP8 (via its PXDLS motif); the interaction is disrupted by binding to adenovirus E1A. Interacts with FOXP1, HIPK2, PNN, NRIP1, MECOM, ZNF366, ZFHX1B and WIZ. Interaction with SATB1 (non-acetylated form); the interaction stabilizes its attachment to DNA and promotes transcription repression. Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Oxidoreductase; Nuclear receptor co-regulator; EC 1.1.1.-; Transcription, coactivator/corepressor; Cell cycle regulation

Chromosomal Location of Human Ortholog: 4p16

Cellular Component: nucleoplasm; transcription factor complex; transcriptional repressor complex; cytosol; nucleus

Molecular Function: protein C-terminus binding; protein domain specific binding; protein binding; protein homodimerization activity; transcription factor binding; NAD binding; transcription factor activity; oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor

Biological Process: negative regulation of cell proliferation; positive regulation of histone deacetylation; transcription, DNA-dependent; regulation of cell cycle; white fat cell differentiation; viral genome replication; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; Golgi organization and biogenesis; protein amino acid phosphorylation; negative regulation of histone acetylation

Disease: Wolf-hirschhorn Syndrome