KLHL12 cdna clone
KLHL12 cDNA Clone
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the KLHL (Kelch-like) family of proteins. This protein has been identified as an autoantigen in the autoimmune disease Sjogren's syndrome and as a potential biomarker in primary biliary cirrhosis. This protein may act as a substrate adaptor of the Cullin-3 ubiquitin ligase complex to promote substrate-specific ubiquitylation. Ubiquitylation by this complex has been shown to regulate the Wnt signaling pathway as well as COPII vesicle coat size. A pseudogene has been identified on chromosome 22. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]
Uniprot Description
KLHL12: Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that acts as a negative regulator of Wnt signaling pathway and ER-Golgi transport. The BCR(KLHL12) complex is involved in ER-Golgi transport by regulating the size of COPII coats, thereby playing a key role in collagen export, which is required for embryonic stem (ES) cells division: BCR(KLHL12) acts by mediating monoubiquitination of SEC31 (SEC31A or SEC31B). As part of the BCR(KLHL12) complex, also acts as a negative regulator of the Wnt signaling pathway by mediating ubiquitination and subsequent proteolysis of DVL3. The BCR(KLHL12) complex also mediates polyubiquitination of DRD4, without leading to degradation of DRD4. Component of the BCR(KLHL12) E3 ubiquitin ligase complex, at least composed of CUL3 and KLHL12 and RBX1. This complex interacts with DVL3 upon activation of the Wnt signaling pathway by WNT3A. Interacts with DRD4, KLHL12 and SEC31A. Ubiquitously expressed. Highly expressed in testis and at lower levels in the submandibular salivary gland. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Ubiquitin conjugating system
Chromosomal Location of Human Ortholog: 1q32.1
Cellular Component: cytosol; ER to Golgi transport vesicle; intracellular membrane-bound organelle; microtubule organizing center
Molecular Function: identical protein binding; protein binding; ubiquitin-protein ligase activity
Biological Process: COPII coating of Golgi vesicle; ER to Golgi vesicle-mediated transport; protein monoubiquitination; Wnt receptor signaling pathway
Research Articles on KLHL12
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Product Notes
The KLHL12 klhl12 (Catalog #AAA1271450) is a cDNA Clone and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: atgggaggca ttatggcccc caaagacata atgacaaata ctcatgctaa atccatcctc aattcaatga actccctcag gaagagcaat accctctgtg atgtgacatt gagagtagag cagaaagact tccctgccca tcggattgtg ctggctgcct gtagtgatta cttctgtgcc atgttcacta gtgagctctc agagaagggg aaaccttatg ttgacatcca aggtttgact gcctctacca tggaaatttt attggacttt gtgtacacag aaacagtaca tgtgacagtg gagaatgtac aagaactgct tcctgcagcc tgtctgcttc agttgaaagg tgtgaaacaa gcctgctgtg agttcttaga aagtcagttg gacccttcta attgcctggg tattagggat tttgctgaaa cccacaattg tgttgacctg atgcaagcag ctgaggtttt tagccagaag cattttcctg aagtggtaca gcatgaagag ttcattcttc tgagtcaagg agaggtggaa aagctaatca agtgcgacga aattcaggtg gattctgaag agccagtctt tgaggctgtc atcaactggg tgaagcatgc caagaaagag cgggaagaat ccttgcctaa cctgctacag tatgtgcgga tgcccctact aacccccagg tatatcacag atgtaataga tgctgagcct ttcatccgct gtagtttaca atgcagggat ctggttgatg aagcaaagaa gtttcatctg aggcctgaac ttcggagtca gatgcaggga cccaggacaa gggctcgcct aggagccaat gaagtgcttt tggtggttgg gggctttgga agccagcagt ctcccattga tgtggtagag aaatatgacc ccaagactca ggagtggagc tttttgccaa gcatcactcg taagagacgt tatgtggcct cagtgtccct tcatgaccgg atctacgtca ttggtggcta tgatggccgt tcccgcctta gttcagtgga atgtctagac tacacagcag atgaggatgg ggtctggtat tctgtggccc ctatgaatgt ccgacgaggt cttgctggag ccaccaccct gggagatatg atctatgtct ctggaggctt tgatggaagc aggcgtcaca ccagtatgga gcgctatgat ccaaacattg accagtggag catgctggga gatatgcaga cagcccggga aggtgccgga ctcgtagtgg ccagtggagt gatctactgt ctaggaggat atgacggctt gaatatctta aattcagttg agaaatacga ccctcataca ggacattgga ctaatgttac accaatggcc accaagcgtt ctggtgcagg agtagccctg ctgaatgacc atatttatgt ggtgggggga tttgatggta cagcccacct ttcttccgtt gaagcataca acattcgcac tgattcctgg acaactgtca ccagtatgac cactccacga tgctatgtag gggccacagt gcttcggggg agactctatg caattgcagg atatgatggt aattccctgc taagtagcat tgaatgttat gaccctatca tcgacagctg ggaagtcgtg acatccatgg gaacccagcg ctgtgatgct ggtgtttgtg ttctccgcga gaagtga. It is sometimes possible for the material contained within the vial of "KLHL12, cDNA Clone" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
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