Rabbit anti-Human, Mouse Islet 1 Polyclonal Antibody | anti-ISL1 antibody

Islet 1 Rabbit pAb

Cat. Num. AAA4751719

• Gene Names: ISL1; Isl-1; ISLET1
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay, Immunoprecipitation
• Purity: Affinity purified
• Synonyms: Islet 1; Polyclonal Antibody; Islet 1 Rabbit pAb; Isl-1; ISLET1; anti-ISL1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Expressed in subsets of neurons of the adrenal medulla and dorsal root ganglion, inner nuclear and ganglion cell layers in the retina, the pineal and some regions of the brain.
• Purity/Purification: Affinity purified
• Form/Format: Supplied in 50nM Tris-Glycine (pH7.4), 0.15M Nacl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

• Applicable Applications for anti-ISL1 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), Immunoprecipitation (IP)
• Application Notes: WB: 1:2000; ICC/IF: 1:20; FC/FACS: 1:20; IP: 1:20
• Immunogen: A synthetic peptide of human Islet 1
• Conjugation: Unconjugated
• Modification: Unmodification
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot detection of Islet 1 in Jurkat,K562 cell lysates using Islet 1 Rabbit pAb(1:1000 diluted). Predicted band size:39KDa. Observed band size:39KDa.)

Related Product Information for anti-ISL1 antibody

DNA-binding transcriptional activator. Recognizes and binds to the consensus octamer binding site 5'-ATAATTAA-3' in promoter of target genes. Plays a fundamental role in the gene regulatory network essential for retinal ganglion cell (RGC) differentiation. Cooperates with the transcription factor POU4F2 to achieve maximal levels of expression of RGC target genes and RGC fate specification in the developing retina. Involved in the specification of motor neurons in cooperation with LHX3 and LDB1. Binds to insulin gene enhancer sequences. Essential for heart development. Marker of one progenitor cell population that give rise to the outflow tract, right ventricle, a subset of left ventricular cells, and a large number of atrial cells as well, its function is required for these progenitors to contribute to the heart. Controls the expression of FGF and BMP growth factors in this cell population and is required for proliferation and survival of cells within pharyngeal foregut endoderm and adjacent splanchnic mesoderm as well as for migration of cardiac progenitors into the heart.

NCBI and Uniprot Product Information

• NCBI GI #: 47606423
• NCBI GeneID: 3670
• UniProt Accession #: P61371; P20663; P47894
• Molecular Weight: 349
• NCBI Official Full Name: Insulin gene enhancer protein ISL-1
• NCBI Official Synonym Full Names: ISL LIM homeobox 1
• NCBI Official Symbol: ISL1
• NCBI Official Synonym Symbols: Isl-1; ISLET1
• NCBI Protein Information: insulin gene enhancer protein ISL-1; islet-1; ISL1 transcription factor, LIM/homeodomain
• UniProt Protein Name: Insulin gene enhancer protein ISL-1
• Protein Family: Insulin gene enhancer protein
• UniProt Gene Name: ISL1
• UniProt Synonym Gene Names: Islet-1
• UniProt Entry Name: ISL1_HUMAN

NCBI Description

This gene encodes a member of the LIM/homeodomain family of transcription factors. The encoded protein binds to the enhancer region of the insulin gene, among others, and may play an important role in regulating insulin gene expression. The encoded protein is central to the development of pancreatic cell lineages and may also be required for motor neuron generation. Mutations in this gene have been associated with maturity-onset diabetes of the young. [provided by RefSeq, Jul 2008]

Uniprot Description

ISL1: Binds to one of the cis-acting domain of the insulin gene enhancer.

Protein type: Cell development/differentiation; Nuclear receptor co-regulator; DNA-binding; Transcription factor; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 5q11.1

Cellular Component: nucleoplasm; mitochondrion; cytoplasm; nucleus

Molecular Function: ligand-dependent nuclear receptor binding; zinc ion binding; bHLH transcription factor binding; estrogen receptor binding; chromatin binding

Biological Process: positive regulation of granulocyte macrophage colony-stimulating factor production; positive regulation of interleukin-12 production; positive regulation of interleukin-1 alpha production; negative regulation of transcription from RNA polymerase II promoter; positive regulation of tyrosine phosphorylation of Stat3 protein; negative regulation of estrogen receptor signaling pathway; pancreas development; positive regulation of cell proliferation; ventricular cardiac muscle morphogenesis; neuron fate specification; negative regulation of neuron apoptosis; sensory system development; neural crest cell migration; positive regulation of DNA binding; trigeminal nerve development; pharyngeal system development; axon regeneration; spinal cord motor neuron cell fate specification; positive regulation of insulin secretion; positive regulation of interleukin-6 production; spinal cord motor neuron differentiation; positive regulation of tumor necrosis factor production; positive regulation of interleukin-1 beta production; positive regulation of interferon-gamma production; negative regulation of neuron differentiation; positive regulation of angiogenesis; mesenchymal cell differentiation; visceral motor neuron differentiation; pituitary gland development; negative regulation of inflammatory response; positive regulation of transcription from RNA polymerase II promoter; peripheral nervous system neuron axonogenesis; retinal ganglion cell axon guidance